Gumus, M.Sipahioǧlu, H.M.Paylan, I.C.Erkan, S.2025-05-102025-05-1020071028-888010.3923/pjbs.2007.936.9402-s2.0-33947162482https://doi.org/10.3923/pjbs.2007.936.940https://hdl.handle.net/20.500.14720/6498Although the reverse transcriptase polymerase chain reaction (RT-PCR) procedure is basically simple operation, often it is not possible to achieve optimum results without optimizing the protocols. An RT-PCR method targeting a 200 bp sequence of the CP gene of Apricot Latent Virus (ApLV) was used as a model to improve the detection limit and to compare the behavior of three different plant tissues in a RT-PCR assay. A number of factors should be considered when selecting the optimal system for RT-PCR. Important considerations include the optimal concentrations of MgCl2, dNTP, Taq DNA polymerase enzyme, specific primer and the amount of cDNA for the downstream applications. This study therefore discusses a series of critical PCR parameters and feasible strategies for optimization of RT-PCR detection of ApLV. © 2007 Asian Network for Scientific Information.eninfo:eu-repo/semantics/openAccessAplvOptimizationRt-PcrArticle106N/AQ393694019069893