Kurt-Kizildogan, AslihanAkarsu, NeslihanOtur, CigdemKivrak, ArifAslan-Ertas, NevrozArslan, SevkiSahin, Nevzat2025-05-102025-05-1020221871-52061875-599210.2174/18715206216662103110857482-s2.0-85122243142https://doi.org/10.2174/1871520621666210311085748https://hdl.handle.net/20.500.14720/8198Cetin, Dogan/0000-0002-5733-4007; Mutlu, Dogukan/0000-0003-3259-5822; Topal, Tufan/0000-0001-6550-4662; Otur, Cigdem/0000-0003-3337-7990; Kurt Kizildogan, Aslihan/0000-0002-9323-0993; Akarsu, Neslihan/0000-0002-0593-1504; Konus, Metin/0000-0002-9953-1375; Yilmaz, Can/0000-0002-0028-6614; Kivrak, Arif/0000-0003-4770-2686; Aslan Ertas, Nevroz/0000-0003-3590-5961Background: Marine actinomycetes are among indispensable sources of natural bioactive compounds with unique antimicrobial and anti-cancer activities. Objective: Herein, it was aimed to elucidate the bioactive potential of a marine-derived Streptomyces ovatisporus Methods: Streptomyces ovatisporus S4702T was cultured in N-Z Amine broth, and extraction was carried out using different organic solvents. Bioassay-guided purification was followed by chemical characterization using NMR and LC-MS/MS. The compound was then evaluated for its antibacterial, antioxidant and cytotoxic activities. Results: Etyl acetate extracts gave the highest antibacterial activity, and chemical characterization of this extract indicated the formula as C15H29O5N3 and the corresponding possible molecular structure as 4H-chromen-4-one derivative. It was found highly potent against Bacillus subtilis ATCC 6633 (MIC: 0.25 mu g ml-1) and Micrococcus luteus ATCC 9341 (MBC: 0.5 mu g ml-1). It has no remarkable antioxidant activity, but a higher EC50 value and less cytotoxicity against normal cells. The EC50 values of this chromen derivative were found as 9.68 mu g ml-1 for human colon carcinoma, 9.93 mu g ml-1 for human prostate adenocarcinoma and 25.5 mu g ml-1 for human embryonic kidney cells. Conclusion: Overall, the presented 4H-chromen-4-one derivative is a remarkable bioactive compound with potent antibacterial and cytotoxic activity. With its high bioactive potential, it is proposed as a good candidate in medicine.eninfo:eu-repo/semantics/closedAccessStreptomyces Ovatisporus S4702 T4H-Chromen-4-OneAntibacterial ActivityNmrLc-MsMsCytotoxicityArticle223Q3Q336237033719978WOS:000754261000006