Yuksel, M. B.Kavak, S.Gecit, I.Basel, H.Gumrukcuoglu, H. A.Demir, H.Meral, I.2025-05-102025-05-1020120100-879X1414-431X10.1590/S0100-879X20120075000752-s2.0-84864650451https://doi.org/10.1590/S0100-879X2012007500075https://hdl.handle.net/20.500.14720/1506Gumrukcuoglu, Hasan Ali/0000-0002-5972-9870; Yuksel, Mehmet Bilgehan/0000-0002-5968-7981The objective of this study was to evaluate the effect of short-term levosimendan exposure on oxidant/antioxidant status and trace element levels in the testes of rats under physiological conditions. Twenty male Wistar albino rats were randomly divided into two groups of 10 animals each. Group 1 was not exposed to levosimendan and served as control. Levosimendan (12 mu g/kg) diluted in 10 mL 0.9% NaCl was administered intraperitoneally to group 2. Animals of both groups were sacrificed after 3 days and their testes were harvested for the determination of changes in tissue oxidant/antioxidant status and trace element levels. Tissue malondialdehyde (MDA) was significantly lower in the levosimendan group (P < 0.001) than in the untreated control group and superoxide dismutase and glutathione peroxidase (GSH-Px) levels were significantly higher in the levosimendan group (P < 0.001). Carbonic anhydrase, catalase and GSH levels were not significantly different from controls. Mg and Zn levels of testes were significantly higher (P < 0.001) and Co, Pb, Cd, Mn, and Cu were significantly lower (P < 0.001) in group 2 compared to group 1. Fe levels were similar for the two groups (P = 0.94). These results suggest that 3-day exposure to levosimendan induced a significant decrease in tissue MDA level, which is a lipid peroxidation product and an indicator of oxidative stress, and a significant increase in the activity of an important number of the enzymes that protect against oxidative stress in rat testes.eninfo:eu-repo/semantics/openAccessLevosimendanOxidative StressReactive Oxygen SpeciesRat TestesSodGsh-PxMdaArticle458Q3Q371672022584643WOS:000307713400005