Acar, IsmailKarabiyik, Halide2026-01-302026-01-3020252335-31122335-395310.20315/ASetL.137.1https://doi.org/10.20315/ASetL.137.1https://hdl.handle.net/20.500.14720/29627In this study, Resupinatus rouxii Consiglio & Setti was collected from Canakkale province, Tiirkiye, and identified using both morphological and molecular data. Macroscopically, the species is characterised by its small size, kidney-shaped pileus, light grey colour, and slightly hairy surface. Microscopic examination confirmed diagnostic features of the genus Resupinatus, and detailed evaluations were made of the basidia, basidiospores, and cystidia. The internal transcribed spacer (ITS) region of nuclear ribosomal DNA was sequenced and used in phylogenetic analyses. The resulting ITS sequence showed more than 99% similarity to the reference sequence of R. rouxii, supporting the morphological identification. This study represents the second scientific report of R. rouxii worldwide and provides the fourth known record of this species, extending its known distribution to Tiirkiye (Canakkale province) and contributing valuable data to its biogeography.eninfo:eu-repo/semantics/openAccessResupinatusFungiNew RecordInternal Transcribed Spacer RegionMolecular PhylogenyTurkeyArticle