Cimen, C.Demir, H.Gur, A.Kubilay, S.2025-05-102025-05-1020070973-12452-s2.0-39649095181https://hdl.handle.net/20.500.14720/18102Polyphenol oxidase (PPO) was purified from Igdir apricot, with a 367 fold purification of PPO by affinity chromatography being achieved. Amount of the protein was determined according to Bradford method. Vmax and Km values were found by means of Lineweaver- Burk graphs. Asetly salisilic acid, paracetamol, ascorbic acid (vitamin C), sodium sulphate, copper sulphate, glucose, sodium nitrite, sodium chlorure, glisine, sodium azide, 2-merkaptoethanol, tyrosine, citric acid, etilendiamin tetra acetic acid (EDTA) ve p-amino benzoic acid were used as inhibitor. Inhibition constants Ki of each inhibitor were found from Lineweaver-Burk graphs. It was found that the p-aminobenzoik acid function showed the highest inhibitory effect.eninfo:eu-repo/semantics/closedAccessCharacterizationInhibitorKineticsPolyphenol OxidasePurificationArticle42N/AN/A659664