Tekpinar, Mustafa2025-05-102025-05-1020180892-70221029-043510.1080/08927022.2018.14318352-s2.0-85041296319https://doi.org/10.1080/08927022.2018.1431835https://hdl.handle.net/20.500.14720/5836Tekpinar, Mustafa/0000-0002-0207-0446Cryo-electron microscopy has become an important tool for protein structure determination in recent decades. Since proteins may exist in multiple conformational states, combining high resolution X-ray or NMR structures with cryo-electron microscopy maps is a useful approach to obtain proteins in different functional states. Flexible fitting methods used in cryo-electron microscopy aim to obtain an unknown protein conformation from a high resolution structure and a cryo-electron microscopy map. Since all-atom flexible fitting is computationally expensive, many efficient flexible fitting algorithms that utilize coarse-grained elastic network models have been proposed. In this study, we investigated performance of three coarse-grained elastic network model-based flexible fitting methods (EMFF, iModFit, NMFF) using 25 protein pairs at four resolutions. This study shows that the application of coarse-grained elastic network models to flexible fitting of cryo-electron microscopy maps can provide fast and fruitful models of various conformational states of proteins.eninfo:eu-repo/semantics/openAccessCoarse-Grained Elastic Network ModelNormal ModeFlexible FittingCryo-Electron Microscopy MapProtein ConformationArticle448Q3Q3688696WOS:000428517900008