Erturk, ElifOnur, Omer EnesAydin, IpekOzel, Mustafa ZaferFirat, MehmetAri, Ferda2025-05-102025-05-1020231516-89131678-432410.1590/1678-4324-20232203582-s2.0-85164611218https://doi.org/10.1590/1678-4324-2023220358https://hdl.handle.net/20.500.14720/10137Aydin, Ipek/0000-0001-5727-9928; Firat, Mehmet/0000-0001-5814-614X; Onur, Omer Enes/0000-0002-2805-8154; Ozel, Mustafa/0000-0003-3585-9117; Ari, Ferda/0000-0002-6729-7908Breast cancer is the leading cause of cancer-related deaths in women throughout the world. Research on natural anti-cancer products from plants has gained traction. Salvia L. species and their derivatives are rare in Turkey and have suggested for their potential anti-cancer effects. The aim of this study is to assess the potential cytotoxic/apoptotic activities of methanol extract of Salvia candidissima Vahl. subsp. candidissima (SCE) on MCF-7 and MDA-MB-231 breast cancer cells. A GCxGC-TOF/MS system and a dual stage commercial thermal desorption injector were used to determine the chemical components of SCE. MTT and ATP viability tests were used to investigate the anti-growth activity. The apoptosis-inducing effect was assessed using a fluorescence staining method. Caspase-cleaved keratin 18 (ccK18, M30-antigen) levels measured by M30-CytoDeath ELISA Kit. The results showed that SCE suppressed the survival of the MCF-7 and MDA-MB-231 breast cancer cells in a dose-dependent manner, based on the findings of both MTT and ATP cell viability tests and pyknotic cell nuclei were observed via fluorescent staining in both cell lines after 48 h of treatment. The treatment group had greater levels of caspase-cleaved keratin 18 in the MCF-7 cells than the untreated group. These results showed that SCE triggers apoptosis, causes cell death in MCF-7 and MDA-MB-231 cell lines. SCE may become promising therapeutic strategy in the treatment of breast cancer with further in vitro and in vivo studies.eninfo:eu-repo/semantics/openAccessSalvia Candidissima VahlSubspCandidissimaBreast CancerMcf-7Mda-Mb-231CytotoxicityArticle66Q4Q2WOS:001019697900001