Asik, GulsahOzdemir, MehmetKurtoglu, Muhammet GuzelYagci, ServerOksuz, LutfiyeGul, MustafaCiftci, Ihsan Hakki2025-05-102025-05-1020141300-01441303-616510.3906/sag-1309-1262-s2.0-84908069063https://doi.org/10.3906/sag-1309-126https://hdl.handle.net/20.500.14720/15739Ozdemir, Mehmet/0000-0002-9316-771X; Oksuz, Lutfiye/0000-0002-6722-141XBackground/aim: beta-Lactamases are an important resistance mechanism in Acinetobacter baumannii. Pseudomonas extended-resistance (PER-1) type beta-lactamase-producing strains have been reported from various geographic locations; however, PER-1 type beta-lactamases from Turkish hospitals have not been investigated extensively. The aim of this study was to determine the prevalence of PER-1 type beta-lactamases in A. baumannii isolates in various regions of Turkey. Materials and methods: A total of 763 clinical A. baumannii isolates were collected from 9 university hospitals and 2 state hospitals between 2008 and 2011. Molecular amplification of the OXA-51 gene from the A. baumannii genome was performed in order to verify identification of the species. Real-time polymerase chain reaction was used to detect bla(PER-1) genes. Results: PER-1 was detected in 24.6% of the isolates. The annual frequencies of the PER-1 enzyme were detected as 52.2%, 35.9%, and 8.3% in 2008, 2009, and 2010, respectively. PER-1 prevalence decreased gradually over time. The differences observed in PER-1 prevalence among the regions of Turkey were statistically significant (chi-square test; P < 0.001). Conclusion: These data demonstrate that the frequency of detection of PER-1 type beta-lactamases in A. baumannii species has decreased in Turkey. However, the increased carbapenem resistance, together with multidrug resistance, has created a worrisome situation regarding this pathogen.eninfo:eu-repo/semantics/openAccessAcinetobacter BaumanniiBeta-LactamasesPer-1Article446Q3Q11041104625552158WOS:000344155900019