Yildiz, GuelsuemSeker Karatoprak, GoekceIlgun, SelenYilmaz, Mustafa AbdullahKose, Yavuz BuelentZengin, Goekhan2025-05-102025-05-1020241126-35041724-557510.1080/11263504.2023.22875872-s2.0-85179998925https://doi.org/10.1080/11263504.2023.2287587https://hdl.handle.net/20.500.14720/9627Zengin, Gokhan/0000-0001-6548-7823; Yilmaz, Mustafa Abdullah/0000-0002-4090-7227; Seker Karatoprak, Gokce/0000-0001-5829-6914; Ilgun, Selen/0000-0002-8544-0683; Gulsum, Yildiz/0000-0002-1697-7380The aerial parts of Helichrysum armenium subsp. armenium were subjected to methanol, ethyl acetate and n-hexane extraction. The extracts were evaluated for their in vitro enzyme inhibitory activity [lipoxygenase (LOX), tyrosinase and elastase], antioxidant activity and toxicity (on fibroblast cells). All the extracts displayed a weak effect on LOX and elastase. The ethyl acetate extract showed the highest inhibition on tyrosinase enzyme with IC50 = 460 mu g/mL, while kojic acid had IC50 = 30 mu g/mL. The methanol and ethyl acetate extracts compared to the n-hexane extract exhibited stronger DPPH center dot and ABTS(center dot +) scavenging activity, as well as iron(II)-chelating potential, and were shown to have rich phenolic and flavonoid contents. The methanol extract was toxic to healthy fibroblast cells at a higher concentration with IC50 > 500 mu g/mL compared to the other extracts. Phytochemical analysis of the extracts was made quantitatively by LC-MS/MS. The major components identified in the methanol and the ethyl acetate extracts were astragalin, quinic acid, apigenin-7-glycoside, isoquercitrin, chlorogenic acid, naringenin, apigenin and luteolin-7-glucoside. The compounds detected in the n-hexane extract were much fewer. Based on these findings, H. armenium subsp. armenium can be considered to be a natural raw material with the potential to be used in the cosmetic industry.eninfo:eu-repo/semantics/closedAccessHelichrysum Armenium Subsp. ArmeniumEnzyme InhibitoryAntioxidantToxicityLc-Ms/MsArticle1581Q3Q2119129WOS:001124654500001