Alpdagtas, SaadetIlhan, ElifUysal, EbruSengor, MustafaUstundag, Cem BulentGunduz, Oguzhan2025-05-102025-05-1020202473-287710.1063/5.00215542-s2.0-85097501897https://doi.org/10.1063/5.0021554https://hdl.handle.net/20.500.14720/6868Alpdagtas, Saadet/0000-0002-8322-2658; Ilhan, Elif/0000-0001-7253-8270; Sengor, Mustafa/0000-0002-2447-7538; Ustundag, Cem/0000-0002-4439-0878; Gunduz, Oguzhan/0000-0002-9427-7574Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the agent responsible for the coronavirus disease of 2019 (COVID-19), which triggers lung failure, pneumonia, and multi-organ dysfunction. This enveloped, positive sense and single-stranded RNA virus can be transmitted through aerosol droplets, direct and indirect contacts. Thus, SARS-CoV-2 is highly contagious and has reached a pandemic level in a few months. Since COVID-19 has caused numerous human casualties and severe economic loss posing a global threat, the development of readily available, accurate, fast, and cost-effective diagnostic techniques in hospitals and in any places where humans spread the virus is urgently required. COVID-19 can be diagnosed by clinical findings and several laboratory tests. These tests may include virus isolation, nucleic acid-based molecular assays like real-time polymerase chain reactions, antigen or antibody-based immunological assays such as rapid immunochromatographic tests, enzyme-linked immunosorbent assays, immunofluorescence techniques, and indirect fluorescent antibody techniques, electrochemical sensors, etc. However, current methods should be developed by novel approaches for sensitive, specific, and accurate diagnosis of COVID-19 cases to control and prevent this outbreak. Thus, this review will cover an overview and comparison of multiple reports and commercially available kits that include molecular tests, immunoassays, and sensor-based diagnostic methods for diagnosis of COVID-19. The pros and cons of these methods and future perspectives will be thoroughly evaluated and discussed.eninfo:eu-repo/semantics/openAccessArticle44Q1Q133305162WOS:000595298200001