Odabas, ÖTimurkan, HAtilla, MKYilmaz, YSengül, EAydin, S2025-05-102025-05-1020000040-87271349-332910.1620/tjem.191.12-s2.0-0034182970https://doi.org/10.1620/tjem.191.1https://hdl.handle.net/20.500.14720/7924Several technical procedures mere described for estimation of epididymal sperm concentration until today. In all of the described techniques, epididymal material was drawn into a micro-pipette before counting procedure. We aimed to proceed this stage without using any pipette because of the difficulty in aspirating very viscous epididymal material into a micro-pipette. For measuring epididymal material we used a 2 mu l reservoir which me named as a micro-spoon. It was filled with the epididymal material and layered in a tube with Ringer's solution of 2 mi containing sodium hyaluronate. Then, it was mixed in order to obtain 1/1000 diluted suspension. Sperm cells in this suspension were counted on a Makler chamber under a light microscope. The procedure was easy and time saving, compared to the other techniques. (C) 2000 Tohoku University Medical Press.eninfo:eu-repo/semantics/openAccessMicro-SpoonCauda EpididymisSperm ConcentrationArticle1911Q3Q21510896034WOS:000087963100001