Ozarslan, MerveBilgili Can, DilberAvcioglu, Nermin HandeCaliskan, Secil2025-05-102025-05-1020221432-69811436-377110.1007/s00784-022-04497-82-s2.0-85128833387https://doi.org/10.1007/s00784-022-04497-8https://hdl.handle.net/20.500.14720/14086Ozarslan, Merve/0000-0001-7158-0952; Caliskan, Secil/0000-0002-8099-584X; Bilgili Can, Dilber/0000-0003-0114-6936; Avcioglu, Nermin Hande/0000-0003-2243-5817Objectives The aim of this study is to evaluate the adhesion of Streptococcus mutans on the surface of CAD/CAM materials with various surface treatments. Methods Vita Enamic, Lava Ultimate, and Cerasmart materials were used in this study. A total of 90 samples were prepared (n=10). After various finishing and polishing procedures (non-polished, manual polished, and glazed), surface roughness (SR) measurements, surface free energy (SFE), and elemental and topographic analysis with FIB-SEM/EDX were used to evaluate the samples' surface properties. To improve bacterial adhesion, CAD/CAM materials were covered with sterile artificial saliva containing mucin for pellicle formation and incubated for 1 h at 37 degrees C. Bacteria were then inoculated into the pellicle-coated specimens, and incubation was performed at 37 degrees C for 24 h. Bacterial adhesion was determined as x 10(5) CFU/mL and monitored using FIB-SEM analysis. The Kolmogorov-Smirnov test was used to statistically analyze the normality of the distribution; the groups were then compared using one-way ANOVA and Tukey's test. Results The SR of the control group was statistically higher in all materials (p < 0.05). There were no statistically significant differences in SR between all materials in the non-polished and manual polished groups (p > 0.05). The Vita Enamic control group exhibited the highest SFE value. The highest S. mutans adhesion was observed in non-polished (p< 0.05). Vita Enamic samples had higher CFU than other groups. Conclusions Non-polished surfaces showed higher SR and bacterial adhesion. Polishing processes affected the surface properties and bacterial adhesion.eninfo:eu-repo/semantics/closedAccessCad/CamBacterial AdhesionPolishingSurface RoughnessSurface TopographyArticle268Q2Q15289529935474553WOS:000788333100001