Conforti, Valeria A.de Avila, David M.Cummings, Nada S.Wells, Kenny J.Uelker, HasanReeves, Jeffy J.2025-05-102025-05-1020070264-410X10.1016/j.vaccine.2007.05.0562-s2.0-34547561125https://doi.org/10.1016/j.vaccine.2007.05.056https://hdl.handle.net/20.500.14720/12499Ulker, Hasan/0000-0002-7776-3925A recombinant ovalbumin-luteinizing hormone-releasing hormone (ova-LHRH) antigen has been developed for immunocontraception. In this study, a novel immunostimulant for ova-LHRH immunization, CpG oligodeoxynucleotide (ODN) 2006, was compared against Mycobacterium butyricum. Also, the immunogenicity of ova-LHRH after lyophilization and exposure to organic solvents was assessed. Rats received either ova-LHRH solubilized in urea; lyophilized ova-LHRH; lyophilized ova-LHRH exposed to methylene chloride; or lyophilized ova-LHRH exposed to ethyl acetate. Immunogenicity of lyophilized ova-LHRH was reduced compared with solubilized ova-LHRH. Exposure to ethyl acetate further decreased immunogenicity of ova-LHRH. CpG ODN 2006 was a more effective immunostimulant than M. butyricum for LHRH immunization. (C) 2007 Elsevier Ltd. All rights reserved.eninfo:eu-repo/semantics/closedAccessLhrh ImmunizationCpg OdnAlycobacterium ButyricumArticle2535Q2Q26537654317643561WOS:000249140300011