Bas, ZehraTurkoglu, Vedat2025-10-302025-10-3020251381-34551744-416010.1080/13813455.2025.25673432-s2.0-105018786673https://doi.org/10.1080/13813455.2025.2567343https://hdl.handle.net/20.500.14720/28747Objective:Glucose-6-phosphate dehydrogenase (G6PD) is an enzyme with many essential biochemical functions. However, in various cancer diseases, increased activity of G6PD causes cancer cells to grow, so G6PD inhibitors have become a significant area of research in cancer treatment.Materials and methods:Here, G6PD was purified 4530-fold with affinity chromatography using 2',5 '-ADP Sepharose 4B from sheep liver. The effects of reduced glutathione (GSH) and ascorbic acid (vitamin C) on G6PD activity were explored.Results and discussion:GSH and ascorbic acid showed a significant inhibitory effect on G6PD, and IC50 values were found as 0.37 mu M and 34.66 mu M, respectively. The inhibition type from Lineweaver-Burk plots of these compounds was identified as non-competitive inhibition. The Ki values of GSH and ascorbic acid were calculated as 0.48 mu M and 30.47 mu M, respectively.Conclusion:In this study, it was observed that GSH and ascorbic acid antioxidant compounds exhibit an inhibitory effect on G6PD and may be protective and preventive against cancer.eninfo:eu-repo/semantics/closedAccessAscorbic Acid (Vitamin C)Glucose-6-Phosphate Dehydrogenase (G6PD)InhibitionPurificationReduced Glutathione (GSH)ArticleQ2Q241055708WOS:001588429600001