Yüksek, VeyselÇetın, SedatUsta, AyşeYaycı, GülayDede, Semiha2025-05-102025-05-1020212667-420310.18038/aubtdc.802794https://doi.org/10.18038/aubtdc.802794https://hdl.handle.net/20.500.14720/18773This study was planned to investigate the effects of glutathione, known to be a strong antioxidant, on caspase-dependent \rapoptosis and oxidative DNA damage in the kidney cells (BHK-21) exposed to high glucose. BHK-21 cell line was cultivated \rby regular passages in vitro conditions. Study groups were set up as control, study and its combinations groups ((Glucose; (285 \rmM, HG), glutathione (250 µM)). After 24 hours of incubation, trypsinized cells were disrupted by freeze-thaw method and \ranalyte was prepared. Caspase 3, 8, 9, M30 and 8-OHdG (oxidative DNA damage marker) levels were determined using \rcommercial ELISA kits. M30, caspase 3, 8 and 9 which are the parameters of apoptosis, were found highest in the HG group. \rIn GSH-treated groups, these parameters decreased slightly. There was no significant difference in the levels of 8-OHdG which \ris the indicator of oxidative DNA damage.eninfo:eu-repo/semantics/openAccessBiyoteknoloji Ve Uygulamalı MikrobiyolojiBiyokimya Ve Moleküler BiyolojiHücre BiyolojisiArticle102N/AN/A132137