Dalar, AbdullahUzun, YusufMukemre, MuzafferTurker, MusaKonczak, Izabela2025-05-102025-05-1020152210-803310.1016/j.hermed.2015.09.0062-s2.0-84951017222https://doi.org/10.1016/j.hermed.2015.09.006https://hdl.handle.net/20.500.14720/15044Konczak, Izabela/0000-0002-1712-9754; Dalar, Abdullah/0000-0002-0080-2519; Mukemre, Muzaffer/0000-0001-6154-6603The phenolic composition of lyophilized hydrophilic extracts obtained from root, stem, leaf and flower of Centaurea karduchorum Boiss. a medicinal plant endemic to Eastern Anatolia (Turkey) used as antidiabetic agent was characterized for the first time. Liquid chromatography mass spectrometry (LC/MS) analysis revealed that luteolin derivatives and chlorogenic acid were the dominating compounds contributing, respectively, from 16.1% (root) to 33.7% (stem) and from 7.3% (flower) to 12.0% (root) of total phenolics. Total reducing capacities of the extracts varied from 24.1 (root) to 38.5 (flower) mg gallic acid Eq./g DW [Folin-Ciocalteu assay] and from 274.0 (root) to 441.0 (stem) mu mol Fe2+/g DW [FRAP assay]. The oxygen radical absorbance capacity (ORAC assay) values varied from 930.5 (root) to 1853.5 (stem) mu mol trolox Eq./g DW. The leaf extract exhibited a pronounced inhibitory activity towards alpha-glucosidase (IC50: 0.63 +/- 0.00 mg/ml) and a weak inhibitory activity towards amylase (IC50: 14.63 +/- 0.67 mg/ml), which suggests a potential to reduce postprandial hyperglycaemia and supports its traditional use as antidiabetic agent. (C) 2015 Elsevier GmbH. All rights reserved.eninfo:eu-repo/semantics/closedAccessCentaurea KarduchorumEastern AnatoliaPhenolic CompoundsAntioxidant ActivityAntidiabetic ActivityArticle54Q3Q2211216WOS:000367107100006