Mahaleh, S.P.G.Arici, O.O.Kivrak, A.Otur, C.Kurt-Kizildogan, A.Mutlu, D.Babat, C.F.2025-10-302025-10-3020252365-654910.1002/slct.2025010902-s2.0-105019197834https://doi.org/10.1002/slct.202501090https://hdl.handle.net/20.500.14720/28811Sulfur-containing compounds exhibit diverse biological activities, including anti-inflammatory, anticancer, antioxidant, antibacterial, and antidiabetic properties. In this study, for the first time, the biological properties of methyl(2-(phenylethynyl)phenyl)sulfane (MPPS), including its antioxidant, antimicrobial, cytotoxic, anti-inflammatory, and anticancer activities, were evaluated. According to the results, MPPS did not show any remarkable antioxidant activity. However, MPPS demonstrated modest antimicrobial activity against S. aureus ATCC 25923 (MIC value: 512 µg/mL) and B. subtilis ATCC 6633 as well as other indicator strains (MIC value: 256 µg/mL). Additionally, the MBC values of MPPS were found to be 512 µg/mL for the two gram-negative strains, E. coli ATCC 25922 and P. aeruginosa ATCC 27853. Furthermore, MPPS showed a dose-dependent cytotoxic effect in all tested cell lines. The EC<inf>50</inf> values of MPPS were determined as 202.68 µM for PANC-1, 226.63 µM for LNCaP, and 166.27 µM for Ishikawa cells, respectively. MPPS significantly inhibited colony formation and induced apoptosis in the cancer cell line. Moreover, MPPS exhibited notable anti-inflammatory activity in RAW264.7 cells. These findings suggest that MPPS has potential for cancer treatment and as an anti-inflammatory and antimicrobial agent. However, further studies are required to confirm these effects. © 2025 Elsevier B.V., All rights reserved.eninfo:eu-repo/semantics/closedAccessAnti-InflammatoryAnticancerAntimicrobialThioanisole DerivativeArticle1039Q3Q3