Khalilzadeh, BalalShadjou, NasrinKanberoglu, Gulsah SaydanAfsharan, Hadide la Guardia, MiguelCharoudeh, Hojjatollah NozadRashidi, Mohammad-Reza2025-05-102025-05-1020180026-36721436-507310.1007/s00604-018-2980-62-s2.0-85052662626https://doi.org/10.1007/s00604-018-2980-6https://hdl.handle.net/20.500.14720/6174De La Guardia, Miguel/0000-0002-9982-1663; Khalilzadeh, Balal/0000-0003-2262-6453; Afsharan, Hadi/0000-0001-7874-2766; Nozad Charoudeh, Hojjatollah/0000-0003-4883-9924Caspase-3 plays a vital role in intrinsic and extrinsic pathways of programed cell death and in cell proliferation. Its detection is an important tool for early detection of some cancers and apoptosis-related diseases, and for monitoring the efficacy of pharmaceuticals and of chemo- and radiotherapy of cancers. This review (with 72 references) summarizes nanomaterial based methods for signal amplification in optical methods for the determination of caspase-3 activity. Following an introduction into the field, a first large section covers optical assays, with subsections on luminescent and chemiluminescence, fluorometric (including FRET based), and colorimetric assays. Further section summarize methods for bioimaging of caspase-3. A concluding section covers current challenges and future perspectives.eninfo:eu-repo/semantics/closedAccessCancerCaspase-3Optical BiosensingBioimagingNanotechnologyBiomedical AnalysisArticle1859N/AQ130159750WOS:000443208600001