Adiyaman, AbdullahBattal, AbdulhamitIlhan, Mert2025-05-102025-05-1020230014-82372065-001910.31925/farmacia.2023.2.62-s2.0-85160059958https://doi.org/10.31925/farmacia.2023.2.6https://hdl.handle.net/20.500.14720/1068Adiyaman, Abdullah/0000-0002-3049-9813The purpose of this study was to investigate the healing effects of an ethanolic wheatgrass (WG) extract obtained from young wheat leaves on a surgically induced endometriosis rat model. 30 female Wistar albino rats with endometriosis were randomly organized into five groups (N = 6): Control, Letrozole a reference drug for endometriosis (0.2 mg/kg/day), WG-25 (25 mg/kg/day WG extract), WG-100 (100 mg/kg/day WG extract) and WG-400 (400 mg/kg/day WG extract). WG-100 extract and Letrozole caused a significantly decrease in adhesion score compared to the control. Additionally, treatment with WG extracts resulted in a significant reduction of endometrial cyst volumes compared to the control group. IL-6 cytokine levels in peritoneal fluid of WG-100 and Letrozole groups were importantly lower than the control. Matrix metallopeptidase 9, VEGF and kinase insert domain receptor genes were expressed in endometrial cysts. According to LC-MS/MS analysis, aconitic acid was the major phenolic acid (54.004 mg analyte/g extract) in the WG extract. Additionally, quinic acid (3.687 mg analyte/g extract) and protocatechuic acid (0.014 mg analyte/g extract) were determined in the WG extract. Moreover, the WG extract had vitamin E (5.49 +/- 0.08 mg/g extract) and vitamin C (7.42 +/- 0.13 mg/g extract). As conclusion, WG extract obtained from young leaves of wheat has ameliorative effects on surgically induced endometriosis due to its rich phytochemical content.eninfo:eu-repo/semantics/openAccessAconitic AcidEndometriosisVitamin CVitamin EWheatgrassArticle712Q4Q2271279WOS:000982169600004