Kurt-Kizildogan, AslihanOtur, CigdemYilmaz, CanArslan, SevkiMutlu, DogukanKivrak, ArifKonus, Metin2025-05-102025-05-1020211570-18081875-628X10.2174/15701808179992011092032262-s2.0-85116036916https://doi.org/10.2174/1570180817999201109203226https://hdl.handle.net/20.500.14720/7446Kivrak, Arif/0000-0003-4770-2686; Konus, Metin/0000-0002-9953-1375; Kurt Kizildogan, Aslihan/0000-0002-9323-0993; Mutlu, Dogukan/0000-0003-3259-5822; Cetin, Dogan/0000-0002-5733-4007Aims: In this study experiments were carried out to explore antioxidant, antimicrobial, cytotoxic properties of novel indole derivative 1-ethyl-2-phenyl-3-phenylethyl-3-thiophen-2-yl-1Hindole (EPI) together with its effect on glutathione S-transferases (GST) activities in human liver carcinoma (HepG2) cells. Background: Indoles probably represent one of the most important heterocyclic structures that have been attracting the interest of many scientists in drug discovery. Objective: The present study was carried out to explore antioxidant, antimicrobial, cytotoxic properties of novel indole derivative 1-ethyl-2-phenyl-3-phenylethyl-3-thiophen-2-yl-1H-indole (EPI) and its effect on glutathione S-transferases (GST) activities in human liver carcinoma (HepG2) cells. Materials and Methods: Pd-catalyst Sonogashira coupling reactions, MTT Assay, Antioxidant capacity test, Antimicrobial test, GST enzyme activity test Results: 1-ethyl-2-phenyl-3-(phenylethynyl)-1H-indole had antioxidant and antimicrobial properties. It displayed significant induction in glutathione S-transferases (GST) enzyme activity in human liver cancer cell lines (HepG2), but cytotoxic effect on all tested cancer cell lines could not be observed. Conclusion: All of these results showed that 1-ethyl-2-phenyl-3-(phenylethynyl)-1H-indole had antioxidant and antimicrobial properties without cytotoxic effect, which could make it a promising active component with further studies..eninfo:eu-repo/semantics/closedAccessIndoleHeteroaromatic CompoundsAntioxidantCytotoxicityAntimicrobialSmall MoleculesSynthesis, Cytotoxicity, Antioxidant and Antimicrobial Activity of Indole Based Novel Small MoleculesArticle185Q4Q4461470WOS:000677488500007