Levent, AbdulkadirYardim, YavuzSenturk, Zuhre2025-05-102025-05-1020140925-400510.1016/j.snb.2014.07.0352-s2.0-84905054117https://doi.org/10.1016/j.snb.2014.07.035https://hdl.handle.net/20.500.14720/15772Levent, Abdulkadir/0000-0001-5792-419X; Yardim, Yavuz/0000-0002-9587-096X; Senturk, Zuhre/0000-0002-0356-9345A novel application of boron-doped diamond electrode is introduced for the determination of ambroxol, a potential antioxidant drug belonging to the expectorant class. The electrochemical oxidation of ambroxol was first investigated by cyclic voltammetry using boron-doped diamond and glassy carbon electrodes in aqueous solutions both with and without the addition of surfactant. The compound was irreversibly oxidized in one/two steps at high positive potentials, resulting in the formation of a couple with a reduction and re-oxidation wave at less positive potentials. Special attention was given to the use of adsorptive stripping voltammetry at a surface of mildly oxidized boron-doped diamond electrode in aqueous solutions over the pH range of 1.0-10.0. Addition of anionic surfactant (sodium dodecylsulfate) to ambroxol-containing electrolyte enhanced the stripping current signal. Using square-wave stripping mode, the drug yielded a well-defined voltammetric response in phosphate buffer pH 2.5 containing 4 x 10(-4) M sodium dodecylsulfate at +1.02V (versus Ag/AgCl) (after 30s accumulation at +0.50V). The process could be used to determine ambroxol in the concentration range of 0.05-0.7 mu M, with a detection limit of 0.010 mu M (4.2 ng mL(-1)). The suggested method was successfully applied to pharmaceuticals and spiked human urine samples. (C) 2014 Elsevier B.V. All rights reserved.eninfo:eu-repo/semantics/closedAccessAmbroxolBoron-Doped Diamond ElectrodeSodium DodecylsulfateAdsorptive Stripping VoltammetryReal SamplesArticle203Q1Q1517526WOS:000341455400069