[Anonymous]2025-05-102025-05-1020050031-94652-s2.0-33746910910https://hdl.handle.net/20.500.14720/4086In order to have a more detailed understanding of the various factors influencing a reverse transcriptase polymerase chain reaction (RT-PCR), a number of important parameters such as Mg+2, primer, enzyme concentration and others were optimized for the detection of Prunus necrotic ringspot virus (PNRSV). Using a PNRSV isolate with a pair of primers, complementary DNA of viral genome as template, and an appropriate enzyme together with magnesium chloride, the following optimal conditions were identified: primer concentration between 0.2 and 0.0002 pmol ml-1 and 0.06-2 units ml-1 for Taq DNA polymerase enzyme for a 50 ml reaction volume when other parameters were optimum; magnesium chloride concentration less than 2.5 mM; dNTP concentration between 1 and 10 mM. The optimum cDNA amount should be 360 ng for a 50 ml reaction mixture. When these optimized concentrations and/or values of the main PCR parameters were brought together for a new RT-PCR, a clear and a reliable PNRSV detection having no background was performed from both growth-chamber and field-grown PNRSV-infected plants.eninfo:eu-repo/semantics/closedAccessArticle442Q2Q2189194WOS:000232720800007