Zengin, AdemCaykara, Tuncer2025-05-102025-05-1020170928-49311873-019110.1016/j.msec.2017.02.0112-s2.0-85011976014https://doi.org/10.1016/j.msec.2017.02.011https://hdl.handle.net/20.500.14720/11936Herein, we have designed a novel multilayer system composed of poly(methyl methacrylate) [poly(MMA)] brush, biotin, streptavidin and protein-A on a silicon substrate to attach on anti-immunoglobulin G (anti-IgG). poly(MMA) brush with vinyl end-group was first synthesized by the interface-mediated catalytic chain transfer polymerization. The brush was then modified with cysteamine molecules to generate the polymer chains with amine end-group via a thiol-ene click chemistry. The amine end-groups of poly(MMA) chains were also modified with biotin units to ensure selective connection points for streptavidin molecules. Finally, a multilayer system on the silicon substrate was formed by using streptavidin and protein-A molecules, respectively. This multilayer system was employed to attach anti-IgG molecules in a highly oriented manner and provide anti-IgG molecular functional configuration on the multilayer. High reproducibility of the amount of anti-IgG adsorption and homogeneous anti-IgG adsorption layer on the silicon surface could be provided by this multilayer system. The multi layer system with protein A may be opened the door for designing an efficient immunoassay protein chip. (C) 2017 Published by Elsevier B.V.eninfo:eu-repo/semantics/closedAccessSurface-Mediated Catalytic Chain Transfer PolymerizationThiol-Ene Click ChemistryPoly(Methyl Methacrylate) BrushesAnti-Immunoglobulin GArticle74N/AN/A10310928254273WOS:000397356900012