Browsing by Author "Çabalar, M"

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Avipoxvirus Infection in Quails
(Tubitak Scientific & Technological Research Council Turkey, 2005) Gülbahar, MY; Çabalar, M; Boynukara, B
The present study describes clinical, virological, histopathological, immunohistochemical and electron microscopic findings of pox infection encountered in a quail flock. Lesions consisted of single or multiple nodules with a crust in variable size of gray to yellow or dark brown discoloration on the comb, eyelids, and the other poorly feathered areas of the body. Histopathological changes consisted of hyperplastic epithelium overlying, with ballooning degeneration of keratinocytes, many of which had eosinophilic intracytoplasmic inclusion bodies (Bollinger bodies), and heterophilic infiltrations admixed with mononuclear cells extending into dermis. Avipoxvirus infection was confirmed by positive immunostaining in the cytoplasm of affected cells for poxvirus antigen, excluded no immunostaining of inclusion bodies, using streptavidin-biotin peroxidase complex method and by demonstration of brick-shaped virus particles with a central core using direct electron microscopy. Typical thickness of chorio-allantoic membranes (CAMs) infected with virus, compared with controls, was observed, and the harvested virus on CAMs was detected as positive against known fowlpox virus using gel diffusion test.
Detection and Rflp Analysis of Canine Parvovirus (Cpv) Dna by Polymerase Chain Reaction (Pcr) in a Dog
(Scientific Technical Research Council Turkey, 2002) Özkul, A; Keles, I; Karaoglu, T; Çabalar, M; Burgu, I
In this study, the detection of canine parvovirus (CPV) in a fecal sample from a dog with enteritis was performed for the first time using the polymerase chain reaction (PCR) in Turkey. The final PCR product was analyzed using the restriction fragment length polymorphysm (RFLP) technique. RFLP analysis using Apa LI and Eco RV restriction endonucleases revealed homology in the nucleotide sequence in at least the VP2 coding region of the virus DNAs detected in the fecal specimen and prepared from attenuated vaccine virus as a positive control.
Prevalence of Rotavirus, Escherichia Coli K99 and O157:h7 in Healthy Dairy Cattle Herds in Van, Turkey
(Tubitak Scientific & Technological Research Council Turkey, 2001) Çabalar, M; Boynukara, B; Gülhan, T; Ekin, IH
A survey was conducted to determine the prevalence of rotavirus, Escherichia coli K99 and O157:H7 in healthy dairy cattle herds in Van and environs. Turkey. Some of the cattle herds had a history of recurrent neonatal diarrhoea for previous years, and even while sampling was being done, some newborn calves were diarrhoeic. Three hundred and twelve faecal specimens from dairy cattle farms free of clinically apparent diarrhoea were tested both for the presence of rotaviruses by PAGE silver staining and for Escherichia coli K99 and O157:H7 by latex agglutination using specific antisera after the growth of each colony. The enteropathogenic characteristic of K99 and O157:H7 isolates was determined by rabbit ileal loop test. No rotavirus was detected from clinically normal cows, heifers or calves. Only 1 of 9 diarrhoeic calves was found to be positive for rotavirus. Out of the 235 E, coli isolates from non-diarrhoeic animals, 28 were positive for K99 and 4 for O157:H7. While 14 of 28 K99 isolates were found to be positive for enteropathogenic Escherichia coli (EPEC), 2 of 4 O157:H7 isolates were also positive for EPEC. An in vitro antibiotic sensitivity test of K99 and O157:H7 isolates indicated that the most sensitive antibiotics were enrofloxacin and danofloxacin.