Browsing by Author "Çelik, I"

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Effects of Propylthiouracil-Induced Hypothyroidism on Plasma Lipid Table in Rabbits
(Tubitak Scientific & Technological Research Council Turkey, 2000) Çelik, I; Türkoglu, V; Yegin, E
Hypothyroidism is commonly associated with hyperlipidemia. The aim of this study was to investigate the effect of Propylthiouracil-induced hypothyroidism on the plasma lipid profile in rabbits. This investigation was performed on male, white New Zealand (Oryctolagus cuniculus huxley) rabbits, Twenty rabbits were used; each of the two groups (hypothyroidism and control) consisted of ten animals, Hypothyroidism was induced by oral administration of Propylthiouracil 50 mg.kg(-1) for three weeks. The mean values of Triiodothyronine (T-3), thyroxine (T-4), free T-3 (FT3) and free T-4 (FT4) were 121.7+/-32.7 ng/dl, 1.81+/-0.60 mu g/dl, 2.36+/-9b92 ng/dl and 0.43+/-0.22 p mol/dl in the control group, and 88.4+/-19.2 ng/dl, 1.15+/-0.37 mu g/dl, 1.48+/-0.44 ng/dl and 0.18+/-0.11p mol/dl in the hypothyroidism group, respectively (p<0.05 for all). Levels of total plasma triglyseride (TG), high-density lipoprotein cholesterol (HDL-C), total cholesterol (TC) and apolipoprotein-B (Apo-B) were recorded as 44.2+/-8.57, 12.2+/-2.7, 40.4+/-14.9 and 87.2+/-19.2 mg.dl(-1) in the control group, and 97.9+/-29.8. 23.2+/-2.9, 67.2+/-13.5 and 147.4+/-55.7 mg.dl(-1) in the hypothyroidism group, respectively (p<0.001 for all parameters). The results showed that T-3, T-4, FT3 and FT4 decreased in the hypothyroidism group, but lipid fractions were higher than in the control group. In addition, a significant negative correlation was found between T-4-TC (r=-0.739, p<0.05) and T-4-Apo-B (r=-0.666, p<0.05).
Effects of Zinc Supplementation To Ration on Some Hematological Parameters in Broiler Chicks
(Humana Press inc, 2002) Dönmez, N; Dönmez, HH; Keskin, E; Çelik, I
The aim of the study was to examine the effects of zinc supplementation on some hematological parameters. Sixty newborn male broiler chicks were utilized in the study. Zinc (Zn) was added into drinking water at levels of 0, 125, 500, and 1000 mg/kg. In the study, there was no significant difference between control and Zn-supplemented groups in erythrocyte count, hemoglobin amount, hematocrit levels, total leukocyte count, and differential leukocyte % levels, but the alpha-naphthyl acetate esterase ANAE(+) lymphocyte rate significantly (p< 0.05) increased in the 125-ppm Zn-supplemented group compared with the control group. In conclusion, the data obtained may be beneficial in demonstrating the effects of zinc on, at least, these parameters.
Purification and Characterization of Acetylcholinesterase From Sheep Liver and Inhibition by Some Painkillers
(Asian Journal of Chemistry, 2006) Güloglu, OF; Türkoglu, V; Çelik, I
In this study, acetylcholinesterase (AChE; EC 3.1.1.7) was purified from sheep liver by affinity chromatography. The purification rate was found 3541.7 fold. The purification control of enzyme was done with sodium dodesilsulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The optimal pH, ionic strength and temperature of enzyme were determined. The optimal pH and the optimum temperature were 7.5-8.5 and 35 degrees C respectively. The highest activity was seen in concentration of 0.2 M (NH4)(2)SO4 as ionic strength. On the other hand, the inhibition effects of some painkiller, (paracetamol + caffein), (neostigmin methylsulfate) and (paracetamol + propifenazon + caffein) were investigated in this study. According to results, the I-50 values are 1.27 X 10(-3), 1.02 x 10(-4) and 1.236 M respectively. Also, K-i values are determined as 1.246 x 10(-3), 4.326 x 10(-5) and 1.646 x 10(-3) mol(-1) min(-1) respectively.