Browsing by Author "Çelik, I."

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Acute Effects of Trichloroacetic Acid on Serum Enzyme Levels and Erythrocyte Carbonic Anhydrase Activity in Rats
(2006) Demir, H.; Çelik, I.
The aim of the present study was to investigate the effects of a sublethal dose of trichloroacetic acid (TCA) on serum enzyme levels and erythrocyte carbonic anhydrase (CA) activity in rats under laboratory conditions. Ten Sprague-Dawley albino rats were divided in 2 groups of 5 rats. A 200 mg/kg dose of TCA was administered intraperitoneally to the 5 rats in the treatment group. An equal amount of saline solution was injected into the control group. Serum enzyme levels and erythrocyte CA activity of the rats were measured 1, 3, and 6 h after treatment. According to the results, while the TCA sublethal dose treatment caused significant increases in lactate dehydrogenase (LDH) and creatine phosphokinase (CPK) after 1 and 3 h, aspartate aminotransferase (AST) significantly increased after 1, 3, and 6 h. No significant differences in the levels of alanine aminotransferase (ALT) and erythrocyte CA were observed in comparison to the controls. It was concluded that TCA may cause toxicity in rats. © TÜBİTAK.
Effects of Nigella Sativa L. on Lipid Peroxidation and Reduced Glutathione Levels in Erythrocytes of Broiler Chickens
(2009) Tülüce, Y.; Özkol, H.; Söǧüt, B.; Çelik, I.
We aimed to determine the effect of Nigella sativa L. on reduced glutathione (GSH) and lipid peroxidation (as malondialdehyde, MDA) in erythrocytes of broiler chickens. We used 100 Ross 308 chickens and they were equally divided into four groups namely control, 0.5 %, 1 % and 1.5 % containing Nigella sativa L. The control group received control broiler fattening feed whereas, the treatments groups were fed by the feed containing 0.5 %, 1 % and 1.5 % grinded Nigella sativa L. seeds for 6 weeks, respectively. Erythrocyte MDA levels were significantly lower in 0.5 % (p<0.002) and 1 % (p<0.005) of Nigella sativa L. groups than in control although GSH levels were significantly (p<0.005) higher in 0.5 % and 1 % of Nigella sativa L. groups. The 1.5 % of Nigella sativa L. did not induce on MDA and GSH levels in the animals. In conclusion, Nigella sativa L. caused protective effects on the oxidative stress-induced erythrocyte injury by inhibiting free radical production and regulation of GSH.
The Effects of Plant Growth Regulators on Activity of Eight Serum Enzymes in Vitro
(Marcel Dekker Inc., 1997) Çelik, I.; Kara, M.
In this study, the effects of plant growth regulators (PGRs). Indole Acetic Acid (IAA), 2,3,5-Triiodo Benzoik Acid (TIBA) and Kinetin on human serum enzymes, myocardial creatine kinase (CK-MB), amylase, creatine kinase (CK), aspartate amino transferase (AST), serum glutamyl pruvic transferase (SGPT). alkaline phosphatase (ALK-P), δ glutamyl transferase (GGT-P) and lactate dehydrogenase (LDH) were investigated (in vitro). I50 values of chemicals caused inhibition were determined by means of activity percentage - [I] diagrams. The results showed that IAA was an activator rather than an inhibitor. It only inhibited AST activity, but increased activities of CK, CK-MB, amylase, ALK-P and LDH. However, IAA ineffected on SGPT and GGT-P. Both TIBA and Kinetin inhibited CK-MB, but either chemicals ineffected on CK, amylase, ALK-P, GGT-P. On the other hand, TIBA ineffected on SGPT and LDH, while Kinetin increased activity of the same enzymes. In conclusion, it was showed that the studied chemicals weakly inhibited enzymes.
The Effects of Some Pesticides on Human and Bovine Erythrocyte Carbonic Anhydrase Enzyme Activities in Vitro
(Marcel Dekker Inc., 1996) Çelik, I.; Çamaş, H.; Arslan, O.; Küfrevioǵlu, Ö.I.
The influence of Penncozeb [Mangan-zinc ethylenediamine-bisdithicarbamate], Dithane, [Manganese etylenebisdithiocarbamate], Cupravit [Cuper oksichloride]. Bayleton [1-(4- chlorophenoxy)-3.3-dimethyl-1-(1H-1.2.4-Thiazol-1-yl)-2.2-butanon], Baythroid [Cyano-(4 fluoro-3-phenoxyphenyl)-methyl-3-(2.2-dichloroethyl -2. 2 metyl - cyclopropancarboxylate], Mavrik [-cyano-3-phenoxybenzyl N-(2-chloro-trifluoro - p -tolyl], Talstar [2-methyl biphenyl-3-ylmethyl(z)-(IRS,3RS)-3-(2-chloro-3.3.3 -triflouroprop -1-enyl)=Dimethylcyclo propenecarboxyate], Endosulfan [6.7.8.9.10.10-hekzachloro-1.5.5a.6.9.9a - hekzahydro-6. 9-methano-2.3.4 - benzole dioxi atehiepin 3-oxide] has been investigated on human erythrocyt carbonic anhydrase isozymes (HCA-I, HCA-II) and bovine erythrocyte carbonic anhydrase (BCA) in vitro. Among the chemicals; Baythroid, Talstar, Mavrik were determined to have inhibition effect, on bovine CA and human CA isoenzymes. Dithane had effect of inhibition only on BCA. The I50 values of chemicals caused inhibition were determined by means of activity percentage [I] diagrams. The values were 2.77×10-3 M. 3.84×10-3 M, 1.56×10-2M, 2.36×10-2 M for BCA, respectively. The values of same chemicals except dithane were 1.67×10-3 M, 4.64×10-3 M, 3.9×10-3 M for HCA-I, and 3.16×10-2 M, 2.81×10-3 M, 1.04×10-2 M for 1HCA-II, respectively. On the other hand, Changes occurred in stock concentration of chemicals showed activator effect were calculated. According to the results reached, 10-2 M cupravit, 4.17×10-2 M endosulfan and 10-2 M bayleton in stock concentration activated on both BCA and HCA-II at different levels. The values activation increases of these chemicals on BCA were 29.90, 46.40, 43.70 %, and 67.11, 57.23, 31.88 % for HCA-II, respectively. Cupravit and bayleton which activated to BCA and HCA-II were ineffective on HCA-I. Values of endosulfan was determined to be 114.40 %. Penncozeb was recorded ineffective for all isozymes. Copyright © 1996 by Marcel Dekker, Inc.
Histopathology and Oxidative Stress in the Liver of Chalcalburnus Tarichi Living in Lake Van, Turkey
(Zhengzhou University, 2014) Kaptaner, B.; Kankaya, E.; Doǧan, A.; Çelik, I.
To assess the environmental quality in the Lake Van, Chalcalburnus tarichi, the only vertebrate and an endemic carp species of this lake basin, samples were collected from the lake, and their livers were examined histologically for this purpose. Histological alterations were recorded and evaluated semi-quantitatively based on the liver lesion categories and their severities. According to the results, five of a total of forty samples displayed normal (healthy) liver architecture, but the others displayed various histopathological alterations, including hepatocyte vacuolization, fatty degeneration, increase in melanomacrophage centers, necrosis, infiltration, congestion, fibrosis, single cell necrosis, bile duct alterations, glycogen depletion and nuclear pleomorphism. Liver index values were calculated and found to be changed between 2 and 50 (≤4 for fish with normal livers (FwNL) and ≥8-50 for fish with histopathological livers (FwHL). We also investigated oxidative stress biomarkers, lipid peroxidation (LPO) and antioxidant enzymes (superoxide dismutase (SOD) and catalase (CAT)) in FwHL as compared with FwNL. LPO and CAT were found to be significantly higher in FwHL, indicating increased oxidative stress. The results revealed that the observed histological and biochemical alterations in the livers of C. tarichi reflect the effects of environmental stressors present in the lake water causing pollution.
Serum Angiotensin-Converting Enzyme and Plasma Atrial Natriuretic Peptide Levels in Hyperthyroid and Hypothyroid Rabbits
(1997) Yeǧin, E.; Yiǧitoǧlu, M.R.; Ari, Z.; Çelik, I.; Akçay, F.; Su̧zek, H.
Background. It is known that serum angiotensin-converting enzyme (ACE) and plasma atrial natriuretic peptide (p-ANP) levels increase in hyperthyroidism. However, the precise mechanism of the effects of thyroid hormone on ANP release remains to be clarified. No study investigating serum ACE together with p-ANP levels has been performed in experimental hyperthyroid and hypothyroid rabbits. The present study was designed in order to provide additional evidence of increased ANP production and secretion in hyperthyroidism and to investigate the relationships between ANP, ACE and thyroid hormones. Methods. Male New Zealand white rabbits (2.3-3.4 kg) were used throughout the study. Hyperthyroidism was induced by daily intraperitoneal administration of L-thyroxin (50 μg/100 g). Hypothyroidism was induced by daily intraperitoneal injection of propylthiouracil (2 mg/100 g body weight). Twelve days after the end of treatment, animals were sacrified under anesthesia and blood samples were obtained from the aorta for serum ACE and thyroid hormone and p-ANP determinations. Results. Serum ACE, plasma renin activity (PRA) and p-ANP were higher in hyperthyroid rabbits and lower in hypothyroid rabbits than in euthyroid rabbits. ANP concentration in atria was lower in hyperthyroid rabbits and higher in hypothyroid rabbits than in euthyroid rabbits. p-ANP, PRA and serum ACE levels were positively correlated with serum thyroxin levels. Inverse correlation was found between serum thyroxin and ANP concentration in atria (a-ANP), and between p-ANP and a-ANP. Conclusions. Our results indicate that not only p-ANP but also serum ACE activity was markedly increased in experimental hyperthyroid rabbits. It was thought that there were both direct and indirect effects of thyroxin on the release of ANP.