Browsing by Author "Çokluk, E."

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Actions of Resveratrol and Serotonin Added To Stored Bloods on Lipid Peroxidation and Oxidative Dna Damage
(Yuzuncu Yil Universitesi Tip Fakultesi, 2018) Huyut, Z.; Şekeroğlu, M.R.; Alp, H.H.; Balahoroğlu, R.; Çokluk, E.
Stored blood for use in diseases such as acute blood loss, injury and anemia can be maintained for about a month. It was reported that some negative changes called "storage lesions" occur in stored bloods time dependently. In order to delay these changes, preservatives are added to stored bloods. In this study, we aimed to determine the effects of resveratrol and serotonin on oxidative DNA and mitochondrial damage and lipid peroxidation in stored blood. Blood was taken from 10 volunteers to different blood bags and each blood was separated into 3 groups (control, resveratrol and serotonin groups). Malondialdehyde (MDA), ubiquinone-10 (CoQ10) and 8-hydroxy-2-deoxyguanosine/106deoxyguanosine (8-OHdG/106dG) levels were determined at baseline and on the 7th, 14th, 21st and 28th day after addition of resveratrol and serotonin. MDA, CoQ10 and 8-OHdG/106dG levels increased over time in the control and serotonin groups (p<0.05). However, resveratrol protected against the time-dependent increase in MDA and CoQ10 values (p>0.05). MDA and CoQ10 levels were significantly lower in the resveratrol-treated group on the 21st and 28th days compared with the other groups (p<0.05). These results show that oxidation sensitivity increased time dependently in stored blood. However, the addition of resveratrol allowed the extension of the blood’s shelf life by protecting against increased lipid peroxidation and oxidized CoQ10. © 2018, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Can the Urine Dipstick Test Be Used as a Proteinuria Screening Test
(Yuzuncu Yil Universitesi Tip Fakultesi, 2024) Batur, T.; Çokluk, E.; Akbay, H.İ.
The present study aimed to evaluate the diagnostic performance of the chemical dipstick protein measurement method, which can be used for screening purposes, in the detection of proteinuria. The results from 795 individuals were retrospectively scanned. In order to evaluate the urine dipstick’s accuracy in detecting proteinuria, the current study accepted protein/creatinine ratio (PCR) as the reference value, and three different criteria were used according to different age groups [PCR: ≥ 0.2 g/g (>2 years), ≥ 0.5 g/g (6 months-2 years), and ≥ 0.8 g/g (0-6 months)]. While evaluating diagnostic sensitivity, specificity, positive predictive value, and negative predictive value, two cut-off values [trace and 1(+)] were evaluated. Area under the curve (AUC) by Receiver operating characteristic analysis were analyzed. The calculated sensitivity levels were lower than 70% for all age groups, with an AUC of 0.680, 0.780, and 0.768 for the age groups of 0–6 months, 6 months–2 years, and > 2 years, respectively. When the PCR was taken as a reference for proteinuria, the chemical dipstick test only proved suitable for screening proteinuria in the >6 months age group. Nonetheless, its performance could benefit from further improvement. Although the use of dipsticks is fast, simple, and inexpensive, this research indicates that they cannot replace PCR and 24-hour urine protein amount tests in the diagnosis and follow-up of renal diseases, which are generally known to be asymptomatic until their progression and complications are detected. © 2024, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Do Avanafil and Zaprinast Change Some Selected Cytokine Levels in Ovariectomized Rat’s Liver
(Yuzuncu Yil Universitesi Tip Fakultesi, 2020) Huyut, Z.; Bakan, N.; Çokluk, E.; Akbay, H.İ.; Alp, H.H.; Şekeroğlu, M.R.
Studies reported that phosphodiesterase-5 inhibitors (PDE-5Is) positively contributed to bone-mineral-density and thickness in rats with ovariectomy, which have the same condition with postmenapozal period. To explain the positive contribution mechanism on bone mineral density of PDE-5Is, we investigated the effect of zaprinast and avanafil on levels of some pro-or anti-resorptive cytokines in ovariectomized-rats. Albino female rats (8 months and 250-350 g) were used and four groups of equal-number were randomly assigned (n=6). Groups; was the sahm operated, positive control (OVX), Zaprinast and OVX, Avanafil and OVX groups, respectively. The levels of Estrogen, IL-1β, IL-6, IL-8, IL-10 and TNF-α were measured by ELISA kits, in liver of rats. IL-1β, IL-6, IL-8 and TNF-α levels were high in groups with OVX compared to sham group, while IL-10 levels were low. Also, IL-1β, IL-6, IL-8 and TNF-α levels were low in zaprinast and especially avanafil-treated groups with OVX and were similar to the sahm group values (p=0.001 for IL-1β, p=0.045 for IL-6, p=0.008 for IL-8, p=0.006 for IL-10, p=0.026 for TNF-α). Zaprinast and especially avanafil inhibited IL-1β, 8 and TNF-α and increased the IL-10 levels compared to the OVX group. This may support opinion that PDE-5Is enhance bone mineralization by inhibiting proresorptive cytokines. © 2020, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
In Stored Human Blood, the Inhibitor Effect of Tannic Acid and Caffeic Acid on Lipid Peroxidation and Oxidative Dna Damage
(Yuzuncu Yil Universitesi Tip Fakultesi, 2016) Huyut, Z.; Şekeroğlu, M.R.; Balahoroğlu, R.; Alp, H.H.; Çokluk, E.
Stored bloods, are exposed to a number of negative changes called as "storage lesions" morphological, biochemical and functional, to begin the first phase of the storage. In this study, we investigated the effects of caffeic and tannic acid in lipid peroxidation and oxidative DNA damage in stored blood. The blood was taken from 10 healthy male individuals to the blood bag containing CPD. Each donor's blood was separated into three groups. The first group was used as the control group with nothing added to the blood. A total of 30 µg/mL caffeic acid and 15 µg/mL tannic acid was added to the second and third group, respectively. Malondialdehyde, ubiquinone-10 and 8-hydroxy-2 deoxyguanosine levels were determined at baseline and on the 7th, 14th, 21st and 28th day, by high pressure liquid chromatography. The MDA and 8-OHdG levels of control and tannic acid groups were increased on the 21st and 28th days (p<0.001), whereas in caffeic acid group was preserved. Also, CoQ10 levels increased in the control group time dependently (p<0.001), but preserved in the other two groups. In addition, CoQ10 and 8-OHdG levels of tannic and especially caffeic acid groups was lower than the control group in the inter-group comparison, in periods of progressing time (p<0.05). These results showed that oxidative stress increased in the stored blood, but adding tannic and caffeic acid into the stored blood can attenuate the effects of oxidation. © 2016, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.