Browsing by Author "Öksüz, E."
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Article Effect of Acetaminophen on Viability of Hela Cells(Yuzuncu Yil Universitesi Tip Fakultesi, 2019) Görgişen, G.; Gülaçar, İ.M.; Öksüz, E.Acetaminophen is a widely used analgesic to release pain and reduce fever. Overdose medication could lead to serious medical conditions such as hepatotoxicity and liver failure. In addition to its cytotoxicity effects, it has an anti-proliferative effect in human cancer cells. In this study it is aimed to determine the apoptotic effect of acetaminophen in HeLa cells. In this perspective, HeLa cells were treated with different concentrations of acetaminophen at three different exposure times. Cell viability was determined by MTT analysis. Results showed that acetaminophen inhibited viability of HeLa cells. Its cytotoxic effect depends on exposure time and concentration. Prolonged exposure of acetaminophen led to decrease in cell survival even at minimum concentrations. © 2019, Yuzuncu Yil Universitesi Tip Fakultesi. All Rights Reserved.Article Plasma Cobalamin Level as a Considered Tumor Marker for Hepatocellular Carcinoma(Yuzuncu Yil Universitesi Tip Fakultesi, 2016) Öksüz, E.; Öksüz, M.; Egesel, T.; Özgür, G.; Saydaoğlu, G.Hepatocellular carcinoma (HCC) is one of the most common cancer among men and women. There are many serological tumor markers for the diagnosis of HCC. These are alpha-fetoprotein (AFP), des-gamma-carboxyprothrombin, vitamin B12 binding protein and HCC associated alkaline phosphatase. The aim of this study was to evaluate the possibility of using vitamin B12 as a tumor marker for HCC. This cross sectional study was performed during a 2 year period, and serum samples were obtained from 38 HCC, 57 non-cancerous cirrhotic and 82 healthy control groups. Vitamin B12 levels were determined by using an automated chemiluminescence system test kit. All HCC patients also had an underlying cirrhotic pattern. The period of the previous liver disease was 30.7±26.3 month in cirrhotic patients and 15.4±10 month in the HCC group. AFP and vitamin B12 levels in HCC patients were significantly higher (median AFP: 219 ng/ml, median B12:1106 ng/ml) than cirrhosis patients (median AFP:9,7 ng/ml, median B12:445 ng/ml) and control group (median B12:442 ng/ml) (p<0,001). In the HCC group, there was a good positive correlation between level of vitamin B12 and AFP (p:0.002) but this correlation was not appeared in cirrhosis group. We also examined whether the correlation between the tumor size and vitamin B12 levels and AFP levels. There was no correlation between these parameters (p>0.05). Vitamin B12 levels can be useful as tumor marker in addition to other tumor markers and imaging modalities. Additional studies should be performed related to this subject and the other liver masses without malignancies. © 2016 Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.Article The Protective Role of Resveratrol on Serum Total Sialic Acid and Lipid-Bound Sialic Acid in Female Rats With Chronic Fluorosis(Yuzuncu Yil Universitesi Tip Fakultesi, 2016) Oto, G.; Ekin, S.; Özdemir, H.; Bulduk, M.; Uyar, H.; Öksüz, E.In the present study, the effect of resveratrol on serum total sialic acid (TSA) and lipid bound sialic acid (LSA) was investigated in the rats exposed to chronic fluoride. The study was administered using 32 female Sprague Dawley rats weighing 200-250 g. Rats were divided into four groups (n=8/group). Group I comprised the control group, group II was treated with sodium fluoride (NaF) (10 mg/L/day), group III was treated with resveratrol (50 mg/L/day) and group IV was treated with NaF+resveratrol for 90 days period. Total sialic acid (TSA) and lipid-bound sialic acid (LSA) were determined in serum samples. Statistical analysis showed that the NaF group was significantly higher than the control group with regards to LSA (17.59±2.734 mg/dL, 12.61±2.013 mg/dL) and TSA (87.86± 8.34 mg/dL, 71.47± 8.57 mg/dL) levels (p<0.01 and p<0.05 respectively). Whereas the Resveratrol group was also significantly lower than the NaF group regarding LSA (13.21±2.848 mg/dL, 17.59±2.734 mg/dL) and TSA (72.44± 10.43 mg/dL, 87.86± 8.34 mg/dL) levels (p<0.05 and p<0.05 respectively), Moreover, no significant differences in LSA (14.62±1.85 mg/dL, 12.61±2.013 mg/dL) and TSA (81.19 ±10.24 mg/dL, 71.47± 8.57 mg/dL) levels were observed in the Resveratrol + NaF groups, as compared to the control group (p>0.05). The present study demonstrated slight positive and beneficial effect of resveratrol on the concentration levels of LSA and TSA in serum. © 2017 Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.