Browsing by Author "Önalan, Ş."

Now showing 1 - 4 of 4

An Alternative Live Food for Fish Larvae; Vinegar Eels (Turbatrix Aceti) Enriched With Bacterial Protein
(Centenary University, 2019) Önalan, Ş.; Sepil, A.
Especially at commercial rearing of marine and ornamental fish species, the achievement of early stage feeding operations is very significant for reducing larval loses. For this reason, it is necessary to produce and use of live food organisms that appropriate for first mouth gap sizes of fish larvae. Widely used in this regard rotifer (Brachionus plicatilis) has got uneasy culture procedures, so evaluating alternative live food organisms is very important. In this study, we have assessed the feasibility of vinegar eels in early stage feeding of altricial fish larvae. Zebra fish (Danio rerio) have got smaller mouth gap size than other fish species have been used in feeding trials and acceptabilities of vinegar eels by larvae was evaluated. And also, an increase in protein contents of vinegar eel evaluated using bacterial proteins (Lactococcus garvieae). © 2019, Centenary University. All rights reserved.
Differences in Ache and Bche Enzyme Activation Levels in Liver and Brain Tissues in Rainbow Trouts Exposed To Different Bacterial Diseases
(Centenary University, 2021) Önalan, Ş.; Çılıngıryeltekın, A.
In this study, symptomatic fish samples were taken from rainbow trout farms. Isolation and identification of agents isolated from fish samples were made. DNA isolations from different purified colonies were carried out with the mericon bacterial DNA kit. Real-Time PCR procedure was performed by using universal bacterial primers. Molecular identifications were performed by blasting the nucleotides obtained by sequence analysis of PCR amplicons. Spectrophotometric measurements were performed at 412 nm wavelengths for AChE activity and 412 nm for BChE activity from liver and brain tissues of fish samples. The activity differences of different disease factors among themselves and according to the control group were examined. As a result of the study, isolation and identification of Bacillus subtilis, Lactococcus garvieae and Staphylococcus epidermidis from 5 different farms were performed. Over 98% similarity was observed as a result of sequencing analysis of the isolates. In this study, it was observed that three different bacteria isolated from trout farms suppressed AChE and BChE enzyme activities in both tissues of trout. © 2021, Centenary University. All rights reserved.
Evaluation of the Potential Use of Oxy-Hydrogen Gas for the Treatment of Lactococcus Garvieae Infected-Zebrafish in Hydrogen-Rich Water Aquarium
(Instituto de Tecnologia do Parana, 2023) Önalan, Ş.; Bulut, M.; Alwazeer, D.
This study aimed to evaluate the use of an oxy-hydrogen generator for preparing hydrogen-rich water (HRW) and studying its effects on the L. garvieae-affected zebrafish. 0, 10, 20, and 100% HRW were prepared to determine the effects of HRW on the mortality rate and gene expression levels of L. garvieae infected-zebrafish. After 48 hours of bacterial injection, the mortality rate of fish was 0, 0, 0, 66, 80, and 100% for the non-infected and non-HRW (G1), infected and 100% HRW (G5), non-infected and 100% HRW (G6), infected and 20% HRW (G4), infected and 10% HRW (G3), and infected without HRW (G2) groups. After 54 hours, there was a non-significant change in immunity-related gene expression levels (IL-1β and IL-6) between non-infected 100% HRW (G6) and non-infected non-HRW control (G1) groups. Gene expression levels were significantly upregulated for IL-1β (14, 13, and 9 times), IL-6 (48, 48, and 22 times), and SOD (9 times for each) genes in G2, G3, and G4 groups, respectively, but not for G5 comparing with control group G1. IL-1β and IL-6 gene expression levels decreased with the increase of hydrogen levels in the water. These results show that HRW could decrease (G3 and G4) and prevent (G5) the mortality of L. garvieae infected-zebrafish. This demonstrates the importance of the application of HRW for the treatment of bacterial infectious diseases of fish using a non-toxic compound as a green method without any residual effects on fish or water. © 2023 by the authors. Submitted for possible open access publication under the terms and conditions of the Creative Commons Attribution (CC BY NC) license (https://creativecommons.org/licenses/by-nc/4.0/).
First Report of Ligula Intestinalis (Cestoda: Pseudophyllidea) in Barbus Ercisianus (Cypriniformes: Cyprinidae) From the Nemrut Crater Lake, Turkey
(Centenary University, 2022) Önalan, Ş.; Atici, A.A.; Sepil, A.; Şen, F.
Van Basin has a rich geographical structure in terms of water resources and endemic fish species. In this study, Ligula intestinalis parasite, which is the host of Barbus ercisianus living in Nemrut Crater Lake, was recorded for the first time. The parasite was described molecularly as well as morphologically. In this context, a primer set was designed for molecular identification for the 28S rRNA gene region of L. intestinalis. Real-Time PCR results with the designed primers gave positive results in all parasite samples. The results were confirmed by a single peak (87) in the HRM analysis. © 2022, Centenary University. All rights reserved.