Browsing by Author "Özdemir, H."

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Antinociceptive Activity of Aqueous Extract of Lepidium Sativum L. in Mice
(Yuzuncu Yil Universitesi Tip Fakultesi, 2015) Özdemir, H.; Yaren, B.; Oto, G.
In the present study the aqueous extract of Lepidium sativum L. (family: Brassicaceae) was investigated for possible antinociceptive effect in Swiss - albino male mice. In this experiment three groups of male mice were used (n=6). Two models were used to study the effects of the extracts on nociception, acetic acid-induced writhing test and hot plate test in mice. Lepidium sativum L. extract was administered in the dose of 20 mg/kg orally 30 minutes prior to pain induction. The aqueous extract showed significant (p<0.05) analgesic activity evidenced by increase in the reaction time by hot plate method and significant (p<0.05) reduction in acetic acid - induced writhings in mice with a maximum effect of 27.00% reduction. These effects were compared with the control and standard drug, diclofenac sodium (50 mg/kg, p.o). The results indicate that aqueous extract of Lepidium sativum L. possesses a significant antinociceptive activity in central and peripheral pain models in mice and therefore, it can be used as supplemental therapy in acute or chronic pain conditions. © 2015, Yuzuncu Yil Universitesi Tip Fakultesi, Universitas Indonesia. All rights reserved.
Investigation of the Neuro-Regenerative Effects of Bioresonance and Magnetotherapy in Sciatic Nerve Damage-Induced Rats
(Universidad de la Frontera, 2023) Tas, A.; Nalci, K.A.; Sincan, S.; Özdemir, H.; Üstün, R.; Aras, I.; Tuncer, M.C.
Peripheral nerve damage is a significant clinical problem that can lead to severe complications in patients. Regarding the regeneration of peripheral nerves, it is crucial to use experimental animals' nerves and use different evaluation methods. Epineural or perineural suturing is the gold standard in treating sciatic nerve injury, but nerve repair is often unsuccessful. This study aimed to investigate the neuroregenerative effects of magnetotherapy and bioresonance in experimental animals with sciatic nerve damage. In this study, 24 female Wistar rats were divided into 7 groups (n=6) as follows: Group 1 (Control), Group 2 (Axonotmesis control), Group 3 (Anastomosis control), Group 4 (Axonotmesis + magnetotherapy), Group 5 (Anastomosis + magnetotherapy), Group 6 (Axonotmesis + bioresonance), Group 7 (Anastomosis + bioresonance). Magnetotherapy and bioresonance treatments were applied for 12 weeks. Behavioural tests and EMG tests were performed at the end of the 12th week. Then the rats were sacrificed, and a histopathological evaluation was made. The statistical significance level was taken as 5% in the calculations, and the SPSS (IBM SPSS for Windows, ver.21) statistical package program was used for the calculations. Statistically significant results were obtained in animal behaviour tests, EMG, and pathology groups treated with magnetotherapy. There was no statistically significant difference in the groups treated with bioresonance treatment compared to the control groups. Muscle activity and nerve repair occurred in experimental animals with acute peripheral nerve damage due to 12 weeks of magnetotherapy, and further studies should support these results. © 2023, Universidad de la Frontera. All rights reserved.
The Protective Role of Resveratrol on Serum Total Sialic Acid and Lipid-Bound Sialic Acid in Female Rats With Chronic Fluorosis
(Yuzuncu Yil Universitesi Tip Fakultesi, 2016) Oto, G.; Ekin, S.; Özdemir, H.; Bulduk, M.; Uyar, H.; Öksüz, E.
In the present study, the effect of resveratrol on serum total sialic acid (TSA) and lipid bound sialic acid (LSA) was investigated in the rats exposed to chronic fluoride. The study was administered using 32 female Sprague Dawley rats weighing 200-250 g. Rats were divided into four groups (n=8/group). Group I comprised the control group, group II was treated with sodium fluoride (NaF) (10 mg/L/day), group III was treated with resveratrol (50 mg/L/day) and group IV was treated with NaF+resveratrol for 90 days period. Total sialic acid (TSA) and lipid-bound sialic acid (LSA) were determined in serum samples. Statistical analysis showed that the NaF group was significantly higher than the control group with regards to LSA (17.59±2.734 mg/dL, 12.61±2.013 mg/dL) and TSA (87.86± 8.34 mg/dL, 71.47± 8.57 mg/dL) levels (p<0.01 and p<0.05 respectively). Whereas the Resveratrol group was also significantly lower than the NaF group regarding LSA (13.21±2.848 mg/dL, 17.59±2.734 mg/dL) and TSA (72.44± 10.43 mg/dL, 87.86± 8.34 mg/dL) levels (p<0.05 and p<0.05 respectively), Moreover, no significant differences in LSA (14.62±1.85 mg/dL, 12.61±2.013 mg/dL) and TSA (81.19 ±10.24 mg/dL, 71.47± 8.57 mg/dL) levels were observed in the Resveratrol + NaF groups, as compared to the control group (p>0.05). The present study demonstrated slight positive and beneficial effect of resveratrol on the concentration levels of LSA and TSA in serum. © 2017 Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Purification and Characterization of Glucose-6 Dehydrogenase From Lake Van Fish (Chalcalburnus Tarichii Pallas, 1811) Erythrocytes
(Chemical Publishing Co., 2007) Özdemir, H.; Türkoglu, V.; Çiftçi, M.
Glucose 6-phosphste dehydrogenase (D-glucose 6-phosphate: NADP+ oxidoreductase, EC 1.1.1.49; G6PD) was purified with 2',5'-ADP Sepharose 4B affinity gel chromatography from Lake Van fish (Chalcalburnus tarichii pallas, 1811) erythrocytes and were investigated some characteristics and kinetics of the enzyme. Purification step of the G6PD were controlled with SDS-PAGE and molecular weight and submolecule was determined by gel filtration chromatography and SDS-PAGE. The activity of enzyme was measured by using Beutler's method. The purification procedure was composed of two steps: hemolysate preparation and 2',5'-ADP Sepharose 4B affinity gel chromatography. The purified enzyme, having the specific activity of 17, 38 EU/mg proteins, was purified 1,100-fold with a yield of 33, 54 %. Optimum pH, optimum temperature and stable pH of the G6PD were 8.5, 40°C and 8.0, respectively. KM and Vmax values for NADP + and glucose 6-phosphate (G6-P) were also determined for the enzyme. For NADP+, KM and Vmax value at optimum pH and 25°C for the G6PD was 0.027 mM and 0.091 EU/mL, respectively. For G6-P, KM and Vmax value at optimum pH and 25°C for the G6PD was 0.0439 mM and 0.013 EU/mL, respectively.