Browsing by Author "Abounaaja, Fatima"

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Genetic Variability of Pestivirus a (bvdv-1) Circulating in Cattle From Eastern Turkey
(Wiley, 2025) Abounaaja, Fatima; Babaoglu, Ali Riza
BackgroundBovine viral diarrhoea virus (BVDV) infection, caused by Pestiviruses A and B, with various clinical findings and causes significant economic losses. This disease is common in Turkey as well as in other countries, especially in European countries.ObjectiveThis study was designed to determine the genotypes of BVDVs and their variability among cattle in eastern Turkey.MethodsA total of 110 samples from 85 cattle suspected of BVDV infection were tested using RT-PCR with primers targeting the 5 ' UTR, autoprotease (Npro) and E2 gene regions of pestiviruses. Sequence and phylogenetic analyses were performed on the 5 ' UTR and Npro gene regions of these samples.ResultsAnalysis of 15 sequences obtained from 13 cattle revealed that Pestivirus A (BVDV-1) was responsible for the infection. In addition, the study identified subgenotypes BVDV-1a (n = 5), 1b (n = 5), 1d (n = 1), 1f (n = 1), 1l (n = 1) and 1r (n = 2). No evidence of infection with Pestivirus B (BVDV-2), Pestivirus D (Border disease virus) or Pestivirus H (HoBi-like virus/BVDV-3) was found.ConclusionThe significance of pestiviruses in causing genital and respiratory problems is once again emphasised, underscoring the necessity of including them in herd screening. Identifying BVDV genetic diversity both in Turkey and worldwide is crucial for developing effective protection, control and eradication strategies, particularly for vaccination programs. As a conclusion, the identification of BVDV-1a, 1b, 1d, 1f, 1l and 1r in the eastern provinces of Turkey points to an increase in BVDV-1 genetic diversity.
Seroprevalence of Bovine Leukemia Virus in Cattle and Buffaloes in the Border Provinces of the Eastern Anatolia Region, Türkiye: Insights Into the Eradication of Infection
(Urmia Univ, 2024) Babaoglu, Ali Riza; Oguz, Fatma Ertas; Yilmaz, Volkan; Coskun, Nuvit; Abounaaja, Fatima
Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis, an oncogenic deltaretrovirus that has emerged as a potential zoonotic infection. The BLV naturally infects cattle and causes economic losses through a slow persistent infection with various clinical symtoms following preleukosis. The main objective of this study was to determine the seroprevalence of BLV antibodies in cattle and buffaloes in the border provinces of the Eastern Anatolia region, T & uuml;rkiye, using the agar gel immunodiffusion (AGID) assay and enzyme-linked immunosorbent assay (ELISA). For this purpose, a total of 1,033 serum samples were collected from 982 cattle and 51 buffaloes from the provinces of A & gbreve;r & imath; (n = 178), I & gbreve;d & imath;r (n = 252), Kars (n = 317), Van (n = 221), and Hakkari (n = 65) during 2021- 2022. In AGID and ELISA tests, seropositivity for BLV-specific antibodies was not detected in cattle and buffaloes from the mentioned provinces. This study revealed that BLV was not circulating in cattle and buffaloes in the easternmost border provinces of T & uuml;rkiye during the sampling period and contributed to determine the status of BLV in the mentioned region. Due to the presence of virus in other regions of T & uuml;rkiye and neighboring countries, Iran and Iraq, it is recommended to control animal movements, continue efforts to combat the transmission of the virus, and maintain control measures. (c) 2024 Urmia University. All rights reserved.