Browsing by Author "Adas, Mine"

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Is Combined Therapy More Effective Than Growth Hormone or Hyperbaric Oxygen Alone in the Healing of Left Ischemic and Non-Ischemic Colonic Anastomoses
(Hospital Clinicas, Univ Sao Paulo, 2013) Adas, Mine; Kemik, Ozgur; Adas, Gokhan; Arikan, Soykan; Kuntsal, Leyla; Kapran, Yersu; Toklu, Akin Savas
OBJECTIVE: Our aim was to investigate the effects of growth hormone (GH), hyperbaric oxygen and combined therapy on normal and ischemic colonic anastomoses in rats. METHODS: Eighty male Wistar rats were divided into eight groups (n = 10). In the first four groups, non-ischemic colonic anastomosis was performed, whereas in the remaining four groups, ischemic colonic anastomosis was performed. In groups 5, 6, 7, and 8, colonic ischemia was established by ligating 2 cm of the mesocolon on either side of the anastomosis. The control groups (1 and 5) received no treatment. Hyperbaric oxygen therapy was initiated immediately after surgery and continued for 4 days in groups 3 and 4. Groups 2 and 6 received recombinant human growth hormone, whereas groups 4 and 8 received GH and hyperbaric oxygen treatment. Relaparotomy was performed on postoperative day 4, and a perianastomotic colon segment 2 cm in length was excised for the detection of biochemical and mechanical parameters of anastomotic healing and histopathological evaluation. RESULTS: Combined treatment with hyperbaric oxygen and GH increased the mean bursting pressure values in all of the groups, and a statistically significant increase was noted in the ischemic groups compared to the controls (p < 0.05). This improvement was more evident in the ischemic and normal groups treated with combined therapy. In addition, a histopathological evaluation of anastomotic neovascularization and collagen deposition showed significant differences among the groups. CONCLUSIONS: Combined treatment with recombinant human growth hormone and hyperbaric oxygen resulted in a favorable therapeutic effect on the healing of ischemic colonic anastomoses.
Levels of Matrix Metalloproteinase-1 and Tissue Inhibitors of Metalloproteinase-1 in Gastric Cancer
(Baishideng Publ Grp Co Ltd, 2011) Kemik, Ozgur; Kemik, Ahu Sarbay; Sumer, Aziz; Dulger, Ahmet Cumhur; Adas, Mine; Begenik, Huseyin; Kotan, Cetin
AIM: To evaluate the levels of preoperative serum matrix metalloproteinase-1 (MMP-1) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in gastric cancer. METHODS: One hundred gastric cancer patients who underwent gastrectomy were enrolled in this study. The serum concentrations of MMP-1 and TIMP-1 in these patients and in fifty healthy controls were determined using an enzyme-linked immunosorbent assay. RESULTS: Higher serum MMP-1 and TIMP-1 levels were observed in patients than in controls (P < 0.001). Serum MMP-1 and TIMP-1 levels were positively associated with morphological appearance, tumor size, depth of wall invasion, lymph node metastasis, liver metastasis, perineural invasion, and pathological stage. They were not significantly associated with age, gender, tumor location, or histological type. CONCLUSION: Increased MMP-1 and TIMP-1 were associated with gastric cancer. Although these markers are not good markers for diagnosis, these markers show in advanced gastric cancer. (C) 2011 Baishideng. All rights reserved.
The Levels of Mmp-2 İn Pancreatıc Juıce From Pancreatıc Adenocarcınoma Patients
(2010) Kemik, Ahu Sarbay; Kemik, Özgür; Dülger, A.cumhur; Adas, Mine; Purisa, Sevim; Tüzün, Sefa; Beğendik, Hüseyin
Pankreas kanseri için erken teşhis edilebilecek ve yüksek riskli hasta grubunda özel bir biomarkır yoktur. Pankreastan salınan proteinlerin bulunduğu zengin bir kaynak olan panreatik sıvı, pancreas kanserinin biyolojik keşfi için ideal bir kaynaktır. 10 pankreatik adenokarsinomalı hasta ve 10 sağlıklı bireyde pancreas suyu alınarak, MMP-2 düzeyleri enzim bağlı immunosorbent ölçüm yöntemi (ELISA) ile belirlendi. Pankreas salgısı MMP-2 düzeyleri, kontrol grubuna göre, pancreas kanserli hastalarda yüksek bulundu (p<0.001). MMP-2 düzeyleri pancreas kanserinin tanımlanmasında ve taramasında yarar sağlayacak, klinik uygulamaya konabilecek bir markırdır.
Serum Levels of Vegf and Egf in Thyroid Gland Tumors
(2010) Purisa, Sevim; Kemik, Ahu Sarbay; Dülger, A.cumhur; Hasırcı, İsmail; Kemik, Özgür; Tüzün, Sefa; Adas, Mine
Amaç: VEGF ve EGF serum düzeyleri troid bezitümörlerinin patogenezinde ve onlarınbüyümesindde rol oynar. Hipotezimiz tiroid tumorhücreleri tarafından üretilen bu sitokininlerperiferal kanda düzeylerini yansıttığınısavunmaktayız.Metod: ELISA kit kullanarak tiroid bezi kanserli20 hastanın ve sağlıklı bireylerimizin periferalkanında bu sitokininleri ölçtük.Bulgular: Biz 10 tiroid adenomalı ve 10 tiroidpapillar karsinomlu hastaların serum VEGF veEGF düzeylerini sağlıklı bireylerimize göre,istatiksel olarak yüksek bulduk. Ancak, tiroidadenomlu ve tiroid papillar karsinomlu hastalararasında ise bu sitokininlerin konsantrasyonlarıarasında bir fark göremedik. Sonuç: Tirodi bezi tumor hücreleri tarafından busitokininlerin üretimindeki değişikliğin peripheralkan düzeylerini yansıttığını düşünmekteyiz. Aynızamanda VEGF ve EGF serum düzeyeleriarasındaki farklılık, tiroid tumor hücreleritarafından üretimini yüksek olması şeklindeaçıklanabilir ve foliküle ve endoteliyal hücreproliferasyonunu güçlü etkisini vurgulamaktadır.