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Browsing by Author "Akan, Mehmet"

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    Isolation of Clostridium Sordellii From Abomasum Lesions of Lambs in Turkey
    (Ankara Univ, 2008) Akan, Mehmet; Sareyyupoglu, Bare; Oncel, Taner; Tel, Osman Yasar; Ilhan, Ziya; Cantekin, Zafer
    In this study, the isolation of C. sordellii from abomasum lesions of lambs and the confirmation of the isolates by polymerase chain reaction (PCR) was attempted. A total of 298 abomasal specimens (swabs) obtained from lambs of 4 to 8 months of age were investigated. Sampled swabs were inoculated onto blood agar plates and incubated anaerobically. Following the incubation grown colonies were identified according to their morphological and biochemical characteristics. C. sordellii was isolated from 17 of 298 specimens (5.7%). These bacteria were also confirmed to harbour C. sordellii DNA by PCR. In conclusion, the presence of C. sordellii in abomasum lesions was demonstrated in Turkey for the first time with this study.
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    Studies on Vaccine Development for Ovine Caseous Lymphadenitis
    (Ankara Univ Press, 2010) Izgur, Mujgan; Akan, Mehmet; Ilhan, Ziya; Yazicioglu, Nahit
    Caseous lymphadenitis (CLA), which is a serious infectious disease of sheep and goats world-wide, has caused significant economic losses in Turkey in recent years. In this study, it was aimed to identify major antigens of Corynebacterium pseudotuberculosis strains isolated in Turkey and to evaluate the efficiency of the vaccine prepared from local strains of C. pseudotuberculosis against bacterial challenge in lambs. C. pseudotuberculosis strains were isolated from six different flocks in Turkey and were classified according to their similarities by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). SDS-PAGE revealed more than 21 bands ranging in size from 15 kDa to 194 kDa present in different concentrations in C. pseudotuberculosis isolates as determined by visual assessment of their approximate molecular masses. Lambs were vaccinated with a bacterin + toxoid + Freund's complete adjuvant (FCA) vaccine, and the vaccine efficiency against bacteriological challenge was evaluated. When a local C. pseudotuberculosis (PI 18) strain was used and formulated as per ml of the vaccine, 1.5x10(8) cfu of C. pseudotuberculosis bacterin + toxoid + 1/2 FCA of the toxoid, we obtained encouraging results regarding the potential of this vaccine to control of CLA in sheep.