Browsing by Author "Akbay, H.İ."

Now showing 1 - 2 of 2

Can the Urine Dipstick Test Be Used as a Proteinuria Screening Test
(Yuzuncu Yil Universitesi Tip Fakultesi, 2024) Batur, T.; Çokluk, E.; Akbay, H.İ.
The present study aimed to evaluate the diagnostic performance of the chemical dipstick protein measurement method, which can be used for screening purposes, in the detection of proteinuria. The results from 795 individuals were retrospectively scanned. In order to evaluate the urine dipstick’s accuracy in detecting proteinuria, the current study accepted protein/creatinine ratio (PCR) as the reference value, and three different criteria were used according to different age groups [PCR: ≥ 0.2 g/g (>2 years), ≥ 0.5 g/g (6 months-2 years), and ≥ 0.8 g/g (0-6 months)]. While evaluating diagnostic sensitivity, specificity, positive predictive value, and negative predictive value, two cut-off values [trace and 1(+)] were evaluated. Area under the curve (AUC) by Receiver operating characteristic analysis were analyzed. The calculated sensitivity levels were lower than 70% for all age groups, with an AUC of 0.680, 0.780, and 0.768 for the age groups of 0–6 months, 6 months–2 years, and > 2 years, respectively. When the PCR was taken as a reference for proteinuria, the chemical dipstick test only proved suitable for screening proteinuria in the >6 months age group. Nonetheless, its performance could benefit from further improvement. Although the use of dipsticks is fast, simple, and inexpensive, this research indicates that they cannot replace PCR and 24-hour urine protein amount tests in the diagnosis and follow-up of renal diseases, which are generally known to be asymptomatic until their progression and complications are detected. © 2024, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Do Avanafil and Zaprinast Change Some Selected Cytokine Levels in Ovariectomized Rat’s Liver
(Yuzuncu Yil Universitesi Tip Fakultesi, 2020) Huyut, Z.; Bakan, N.; Çokluk, E.; Akbay, H.İ.; Alp, H.H.; Şekeroğlu, M.R.
Studies reported that phosphodiesterase-5 inhibitors (PDE-5Is) positively contributed to bone-mineral-density and thickness in rats with ovariectomy, which have the same condition with postmenapozal period. To explain the positive contribution mechanism on bone mineral density of PDE-5Is, we investigated the effect of zaprinast and avanafil on levels of some pro-or anti-resorptive cytokines in ovariectomized-rats. Albino female rats (8 months and 250-350 g) were used and four groups of equal-number were randomly assigned (n=6). Groups; was the sahm operated, positive control (OVX), Zaprinast and OVX, Avanafil and OVX groups, respectively. The levels of Estrogen, IL-1β, IL-6, IL-8, IL-10 and TNF-α were measured by ELISA kits, in liver of rats. IL-1β, IL-6, IL-8 and TNF-α levels were high in groups with OVX compared to sham group, while IL-10 levels were low. Also, IL-1β, IL-6, IL-8 and TNF-α levels were low in zaprinast and especially avanafil-treated groups with OVX and were similar to the sahm group values (p=0.001 for IL-1β, p=0.045 for IL-6, p=0.008 for IL-8, p=0.006 for IL-10, p=0.026 for TNF-α). Zaprinast and especially avanafil inhibited IL-1β, 8 and TNF-α and increased the IL-10 levels compared to the OVX group. This may support opinion that PDE-5Is enhance bone mineralization by inhibiting proresorptive cytokines. © 2020, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.