Browsing by Author "Akbulut, Yeliz"

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Drug Agent Substance Determination With the Shape Memory Paper in Blood Sample
(2018) Akbulut, Yeliz; Zengin, Adem
Bu çalışmada, sentetik kan örnekleri içerisinde ilaç etken madde olan propranolol tayininde kullanılmak üzere moleküler baskılanmış kağıt (Whatman No 1) yüzeyler hazırlanmıştır. Bunun için kağıt yüzeyler ilk olarak tersinir katılma-ayrılma zincir transfer (RAFT) ajanı ile modifiye edilmiştir. Ardından kağıt yüzeyler N-akriloil-L-fenilalanin (NAPAL, monomer), metilen bisakrilamit (MBAAm, çapraz bağlayıcı), propranolol (kalıp molekül), azobis isobutironitril (AIBN, başlatıcı) içeren çözeltiye konularak yüzeyde moleküler baskılama yöntemi ile baskılanmış polimerler hazırlanmıştır. Moleküler baskılanmış kağıt yüzeylere propranolol için maksimum adsorpsiyon kapasitesi 64.3 mg/g olarak belirlenmiş ve bu değere ulaşılması için optimum polimerizasyon süresi 8 saat, propranolol/NAPAL oranı 1 ve polimerizasyon sıcaklığının 60 °C olduğu sonucuna ulaşılmıştır. Kalıp molekül propranololü polimer matrisinden etkin bir şekilde uzaklaştırmak için metanol: asetik asit (5:1, v/v) karışımı en uygun çözücü karışımı olarak belirlenmiştir. Propranolol kalıp moleküler yapıca benzer moleküller (atenolol ve pindolol) ile seçicilik çalışması yapılmış ve kağıt yüzeylerin propranolol'e oldukça seçici olduğu gösterilmiştir. Moleküler baskılanmış kağıt yüzeyler kullanılarak sentetik kan içerisinde propranolol'ün oldukça yüksek geri kazanım oranlarında (% 97.0 - % 99.7) ve düşük % bağıl standart sapma (% 3.78 - % 4.92) ile tayin edilebilceği gösterilmiştir.
Magnetic Nanoparticles Coated With Aminated Polymer Brush as a Novel Material for Effective Removal of Pb(Ii) Ions From Aqueous Environments
(Springer Heidelberg, 2019) Yilmaz, Sakir; Zengin, Adem; Akbulut, Yeliz; Sahan, Tekin
In the present study, a poly (vinylbenzyl chloride) grafted Fe3O4 nanoparticle (Fe3O4@PVBC) was prepared by surface-initiated reversible addition fragmentation chain transfer (SI-RAFT) polymerization and subsequently coated with tris (aminoethyl) amine (TAEA). Then, Fe3O4@PVBC-TAEA nanoparticles were utilized as a novel adsorbent for removal of Pb(II) from aqueous media and optimal adsorption conditions were determined with response surface methodology (RSM). The used adsorbent was characterized by using X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM), and vibrating sample magnetometer (VSM). RSM with central composite design (CCD) was carried out to evaluate the effect of initial pH, initial Pb(II) concentration (C-0, mg/L), adsorbent dosage (mg), and contact time (min). The optimum initial pH, C-0, adsorbent dosage, and contact time were found to be 5.88, 46.51mg/L, 17.41mg, and 108.21min, respectively. The maximum removal efficiency and adsorption capacity were 97.07% and 129.65mg/g under these conditions, respectively. The kinetic data revealed that the adsorption mechanism could be best explained by the pseudo-second-order and Weber-Morris models. The isotherm studies found that both the Langmuir and Freundlich isotherm models fitted the experimental data well. The thermodynamic analysis indicated that the adsorption nature is exothermic, applicable, and spontaneous.
A Molecularly Imprinted Whatman Paper for Clinical Detection of Propranolol
(Elsevier Science Sa, 2020) Akbulut, Yeliz; Zengin, Adem
Determination of drug concentration in body fluids is important issue for clinical studies to arrange treatment of patients. In the present study, we concentrated on the preparation of propranolol-imprinted poly (N-acryloyl-L-phenylalanine) film on a paper surface for spectrophotometric detection of propranolol in human plasma samples. The surface characterization of the imprinted surface was carried out by attenuated total reflectancefourier transform spectroscopy, x-ray photoelectron spectroscopy, scanning electron microscopy and water contact angle measurements. Rebinding isotherms and kinetics were also investigated and maximum adsorption capacity of the imprinted paper surface was found to be 64.3 mg/g with high imprinting factor (4.20). Moreover, the results for selectivity and regeneration ability of the imprinted paper indicated that propranolol selectively interacted with the imprinted paper and had satisfactory reuse without changing its adsorption capacity. Under optimized conditions, the imprinted paper surface had a limit of detection of 0.3 mu g/mL with lower intraday and interday precisions for determination of propranolol. The proposed method was successfully applied to determine propranolol in plasma samples where it showed recoveries ranging from 97.0%-99.5%. The method was also compared with traditional ELISA method and the results showed that the proposed method is sensitive and selective. It is believed that the prepared paper-based molecularly imprinted polymers can be good alternatives to traditional drug assays in clinical practice.