Browsing by Author "Akcay, Mehmet Emin"

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Identification of Self Incompatibility (S) Alleles in Turkish Apple Gene Sources Using Allele-Specific Pcr
(Galenos Publ House, 2023) Karatas, Merve Dilek; Hazrati, Nahid; Yuksel Ozmen, Canan; Hasanzadeh, Mohammad; Altintas, Serdar; Akcay, Mehmet Emin; Ergul, Ali
Self-incompatibility (SI) is a genetic mechanism in many flowering plants by which generative reproduction is prevented. The self-incompatibility caused by the genetic functions of the cell is controlled by genes called S genes or self-incompatibility genes. Self-incompatibility results in decreased pollination and ultimately yield loss. In apple (Malus domestica L.), self-incompatibility is controlled by multi-allelic S-locus. Approaches in the S-glycoprotein profiles and allele-specific PCR methods using the gene profiles and S-glycoprotein profiles for determination of the incompatibility levels are of great importance. In current study, the self-incompatibility status of 192 apple genotypes (such as, Amasya, Huryemez, Sah elmasi, Tokat, Demir elmasi etc.) obtained from the National Collection of Ataturk Horticultural Central Research Institute, Yalova, Turkey, has been determined. For this purpose, genotype-specific allele status and compatibility levels were screened via PCR (Polymerase Chain Reaction) using 4 different S-alleles (Sd, Sf, S26 and S9). 181 genotypes containing at least 1 S-allele were identified as 'Partially Incompatible' and 12 genotypes involving 4 S-alleles were assigned 'Totally Incompatible'. No S-alleles were observed in 2 genotypes (Pancarlik and Huryemez) which exhibited 'Compatibility' status.
Influence of Postharvest Application of 28-Homobrassinolide Onstorage Quality of Medlar Fruit
(Springer, 2019) Ekinci, Neslihan; Gokbayrak, Zeliha; Cavusoglu, Seyda; Akcay, Mehmet Emin
This study was conducted to determine the effects of 28-homobrassinolide (Hbl) applied postharvest on the storage quality of medlar fruit (Mespilus germanicaL.). Fruits were submerged into the solutions containing 3 and 5 mu M Hbl for 5min. Control fruits were submerged into distilled water for the same duration. Treated and control fruits were stored at 0 degrees C and 95% relative humidity conditions for 60days. Samples were taken at 15, 30, 45 and 60th day of storage. On the sampling dates, soluble solid content (SSC), pH, titratable acidity (TA) in malic acid, fruit firmness, weight loss, taste, browning, and color of the skin and pulp were analyzed. Results showed that these properties of the fruits changed over time in the storage and depended on the concentration of the homobrassinolide. 3 mu M Hbl treated fruits kept their firmness and initial weight longer. Fruits with 5 mu M Hbl application resulted in high TA and low SSC and provided best browning in the fruit skin. Taste was better attained and protected over alonger time with 5 mu M Hbl treatment. In conclusion, 28-homobrassinolide exerted some effects on quality of medlar fruits.