Browsing by Author "Akkol, Esra Kuepeli"

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Sedative Effects of Latexes Obtained From Some Lactuca L. Species Growing in Turkey
(Mdpi, 2020) Ilgun, Selen; Akkol, Esra Kuepeli; Ilhan, Mert; Polat, Derya Cicek; Kilic, Ayse Baldemir; Coskun, Maksut; Sobarzo-Sanchez, Eduardo
Lactuca L. species belong to the Asteraceae family and these plants are traditionally used for therapeutic purposes around the world. The dried milky latex of L. serriola is known as "lettuce oil" and is used as a sedative in Turkey. This study aimed to evaluate the sedative effects and analyze the chemical compositions of latexes obtained from some Lactuca species growing in Turkey. The sedative effects were evaluated through various behavioral tests on mice. For this purpose, latexes were obtained from L. glareosa Boiss., L. viminea (L.) J. Presl and C. P, L. mulgedioides (Vis and Pani) Boiss. and Kotschy ex. Boiss., L. saligna L., and L. serriola L. The latex from L. saligna showed the highest sedative effects, whilst L. serriola and L. viminea latexes also displayed significant sedative effects compared to the control group at a dose of 100 mg/kg. However, the latexes from L. glareosa and L. mulqedioides did not exhibit any sedative effects on mice. Characteristic sesquiterpene lactones (lactucin, lactucopicrin, 11,13 beta-dihydrolactucin, and 11,13 beta-dihydrolactucopicrin) were determined qualitatively and quantitatively by high-performance liquid chromatography (HPLC). Lactucin was identified as the main component.
Wound Healing Acceleration and Anti-Inflammatory Potential of Prunella Vulgaris L.: From Conventional Use To Preclinical Scientific Verification
(Elsevier Ireland Ltd, 2022) Akkol, Esra Kuepeli; Renda, Gulin; Ilhan, Mert; Bektas, Nurdan Yazici
Ethnopharmacological relevance: The genus Prunella L. (Lamiaceae) is represented by nine species in the world and four species in Turkey. The infusion prepared from the aerial parts of Prunella vulgaris L. is used internally for abdominal pain and as an expectorant, the decoction prepared from all parts is used internally or externally as a wound healing. Aim of the study: This study aims to investigate the wound healing potential of Prunella vulgaris L. on the scientific platform. Material and methods: The aerial parts of the plant were extracted with 80% methanol. The resulting aqueous methanol extract was partitioned with n-hexane and ethyl acetate, and sub-extracts were obtained. The wound healing effects of the methanol extract and sub-extracts were studied in mice and rats using linear incision and circular excision wound models, and the anti-inflammatory effect was investigated using acetic acid-induced capillary permeability test. Isolation studies were performed using the ethyl acetate sub-extract, which exhibited the highest activity. Results: Using various chromatographic methods, 6 compounds were isolated from the ethyl acetate sub-extract. The structures of the compounds were identified as methyl arginolate, ursolic acid, chlorogenic acid, rosmarinic acid, methyl 3-epimaclinate, and ethyl rosmarinate by spectroscopic techniques (UV, IR, 13C-NMR, 1H-NMR, 2D-NMR, MS). The wound healing mechanisms of the pure compounds were investigated by performing assays to inhibit the enzymes hyaluronidase, collagenase, and elastase. Ursolic acid, chlorogenic acid, and rosmarinic acid were found to be responsible for the anti-inflammatory and wound healing effects. Conclusion: The experimental study revealed that Prunella vulgaris showed significant wound healing and antiinflammatory activities.