Browsing by Author "Aksakal, A"
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Article Aeromonas Hydrophila Associated With Ovine Abortion(Elsevier Science Bv, 2006) Ilhan, Z; Gülhan, T; Aksakal, AA sheep farm housing 124 animals, of which 68 were ewes having history of abortion and placenta retention, were informed. In total, 19 vaginal swabs taken from aborted ewes, 3 fetuses, 4 placentas, and I drinking water sample were investigated microbiologically. A total of 60 blood samples (27 from aborted ewes, 18 from pregnant ewes and 15 from healthy neonate ewes) were investigated serologically. Eighteen Aeromonas hydrophila strains were isolated: 13 from vaginal swabs, 3 from fetuses, I from placenta and I from drinking water. Protein profiles of A. hydrophila strains were determined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Of the 27 aborted ewes sera, 7 sera were found to be positive in rapid agglutination test (RAT) with prepared RAT antigen from A. hydrophila. A. hydrophila strains were determined to be resistant to penicillin G, erythromycin and gentamicin. The most effective antibiotics were amoxycillin and enrofloxacin. A. hydrophila is likely to interfere with the normal development of the fetus, especially if pregnancy and other stressing factors occur during gestation. (c) 2005 Elsevier B.V. All rights reserved.Article Brucellosis in Horses Around Van, Turkey(indian veterinary Journal, 2004) Solmaz, H; Tutuncu, M; Akkan, HA; Aksakal, A; Gulhan, T; Boynukara, BA programme is being carried out in Turkey to eradicate brucellosis from cattle by compulsory slaughtering of positive cases on serological test surveillance. In spite of the very strict application of the programme, the results obtained so far are not satisfactory in respect of eliminating the disease. It seems that it will be very difficult to achieve this goal without considering the disease status in other domestic animals. Brucella abortus is associated with chronic inflammatory conditions including fistulous withers, poll-evil and also joint infection in horses. Consequently, in this study, to evaluate prevalence of brucellosis in horses, a comparative study of standard tube agglutination test (STAT) and Rose Bengal Plate Agglutination Test (RBPT) were carried out on horse sera.Article Detection of Campylobacter Antibodies in Sheep Sera by a Dot-Elisa Using Acid Extracts From C-Fetus Ssp Fetus and C-Jejuni Strains and Comparison With a Complement Fixation Test(Wiley, 2002) Gürtürk, K; Ekin, IH; Aksakal, A; Solmaz, HIn this study, a dot-enzyme-linked immunosorbent assay (Dot-ELISA) was evaluated in comparison with a complement fixation test (CFT) for the detection of Campylobacter antibodies in sheep sera. Acid glycine extracts (AGE) of both Campylobacter ssp. fetus and Campylobacter jejuni strains that had been isolated from the gall-bladder of slaughtered sheep was used as antigen in both tests. A total of 153 sheep sera from aborted (74) and slaughtered (79) sheep were examined by both Dot-ELISA and CFT. Twenty-two sera showed anti-complementary activity were not suitable for CFT. Of the 22 sera showing anti-complementary activity, two sera were found to be positive in Dot-ELISA. Eighty-eight (67.2%) of the remaining 131 sera were negative by both Dot-ELISA and CFT using AGE of both Campylobacter strains whereas 43 sera (32.8%) gave different reaction patterns in Dot-ELISA and CFT with the extracts of both Campylobacter strains. Twelve sera were positive by both tests using AGE of C. fetus ssp. fetus but CFT failed to detect antibodies in nine of these sera when AGE of C. jejuni was used. Twelve sera were positive by both tests only when AGE of C. fetus ssp. fetus was used. Eleven sera were positive only, by CFT. Seven of these reacted only with the AGE of C. fetus ssp. fetus and four sera were positive by using AGE of both Campylobacter strains. The remaining eight sera were found to be positive only by dot-immunobinding assay either with the AGE of both Campylobacter strains or with the AGE of one of the Campylobacter strains. It is concluded that Dot-ELISA using AGE from C. fetus ssp. fetus could be employed for the detection of Campylobacter antibodies in sheep sera and the additional use of AGE from C. jejuni as antigen appeared not to be profitable for this purpose.Article Investigation on Carriage of Clostridium Difficile in Van Cats(indian veterinary Journal, 2003) Solmaz, H; Aksakal, A; Tutuncu, M; Akkan, HA; Boynukara, BArticle Seroprevalence of Brucellosis in Stray Dogs in the Region of Van, Turkey(indian veterinary Journal, 2004) Solmaz, H; Tutuncu, M; Akkan, HA; Boynukara, B; Aksakal, A