Browsing by Author "Aksoy, Hasan Murat"

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A 2,2-Dichloropropionic Acid-Degrading Novel Pseudomonas Fluorescence Strain Fatsa001: Isolation, Identification, and Characterization
(Taylor & Francis inc, 2025) Meral, Ulku Binboga; Edbeib, Mohamed Faraj; Kirkinci, Suleyman Faruk; Aksoy, Hasan Murat; Akman, Ayhan; Wahab, Roswanira Abdul; Kaya, Yilmaz
There are mounting concerns over the high concentrations of non-biogenic, toxic halogenated organic compounds being liberated into the ecosystem. Therefore, this study's isolation of a novel bacterium from a contaminated stream in Fatsa, Ordu, Turkey, adept in degrading 2,2-dichloropropionic (of 2,2-DCP) is a welcome endeavor. The ability of the bacterial isolate to utilize 2,2-DCP as the sole carbon and energy source was discovered when the bacterium was observed to grow well on liquid minimal media containing 20 mM of 2,2-DCP, showing a doubling time of 14.2 h. The following genetic and biochemical characterizations revealed that the 16S rRNA sequence of the fatsa001strain is identical (99%) to Pseudomonas fluorescence, after which the sequence was deposited in the NCBI GenBank as Pseudomonas sp. strain fatsa001 (MN098848). The halogen-degrading ability of the P. fluorescens fatsa001 bacterium was again confirmed by the PCR data, which showed the presence of a conserved group of amino acids from the group I dehalogenase gene. It worth mentioning here that this is the first report on a P. fluorescence bacterial strain with the ability to degrade toxic 2,2-DCP. The detoxification ability of this bacterium envisages its practicality as an in situ environmental bioremediation agent.
Agrobacterium- Mediated Transformation of Turkish Upland Rice (Oryza Sativa L.) for Dalapon Herbicide Tolerance
(Natl inst Science Communication-niscair, 2020) Kaya, Yilmaz; Aksoy, Hasan Murat; Edbeib, Mohamed Faraj; Wahab, Roswanira Abdul; Ozyigit, Ibrahim Ilker; Hamid, Azzmer Azzar Abdul; Aslan, Ali
Agrobacterium-mediated transformation of upland rice is established in few numbers of cultivars due to the high cultivar-specificity of regeneration from transformed explants. Further, dehalogenase E (dehE) gene had been characterized in Pseudomonas putida and it produces an enzyme that degrades dalapon. This study aimed to transform Turkish upland rice with the dehE herbicide resistant gene and addresses the challenges of transgenic rice recovery by identifying explant and transformation method. Constructed vector pCAMdehE carrying dehE gene was transferred into the rice shoot apex by Agrobacterium-mediated transformation. The transformed rice was analyzed for expression of the transgenes by polymerase chain reaction (PCR). Herbicide resistance leaf painting assay was carried out at different dalapon herbicide concentrations to the transgenic rice leaves. Transformation efficiency percentage (putative) was highest (32.66%) in 5 days old explants. PCR analysis resulted in the amplification of the dehE, T-DNA border endonuclease (virD2) and hygromycin phosphotransferase (hpt) genes from the transgenic rice. In addition, dehalogenase activity was proved with higher dalapon tolerance in the rice. Dalapon effects started to appear in the transformed rice at 180 mg/l, while in non-transformed ones at 60 mg/l concentration. The results showed that transformed plants have more tolerance to the herbicide than the non-transformed ones.