Browsing by Author "Aksu, D. S."

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Effect of Pomegranate (Punica Granatum L.) Juice on Kidney, Liver, Heart and Testis Histopathological Changes, and the Tissues Lipid Peroxidation and Antioxidant Status in Lead Acetate-Treated Rats
(C M B Assoc, 2017) Aksu, D. S.; Saglam, Y. S.; Yildirim, S.; Aksu, T.
Pomegranate juice (PJ) contains relevant amounts of active biological compounds which alleviate the detrimental effects of chronic heavy metal exposure. This study investigated the protective potential of PJ against lead-induced oxidative stress. A total of forty adult male Sprague Dawley rats were divided into four experimental groups. The animals were fed a standard pellet diet and tap water ad libitum. The rats were divided into four groups (n=10 for each group): control, lead asetat (2000 ppm), low-treated PJ- a daily dose of 2.000 ppm lead plus 30 mu l pomegranate juice (included 1.050 mu mol total polyphenols, gallic acid equivalent), and high-treated PJ- a daily dose of 2.000 ppm lead plus 60 mu l pomegranate juice (included 2.100 mu mol total polyphenols, gallic acid equivalent). The treatments were delivered for 5 weeks. After the treatment period, the tissues samples (kidney, liver, heart and testis) were collected. Tissue lead (Pb) and mineral amounts (copper, zinc, and iron), tissues lipid peroxidation level and antioxidant status, and tissues histopathological changes were determined. The results showed that the highest rate lead loading was in the kidney and the testis. Pomegranate juice was decreased the lead levels of soft tissues examined; increased Zn amounts in tissues of which the lead accumulation was higher (kidney and the testis); decreased the copper, zinc and the iron levels of the liver and heart tissues, without creating a weakness in antioxidant capacity of these tissues, restricted the oxidative stress by decreasing lipid peroxidation, improved both of the activities of antioxidant enzymes such as superoxide dismutase (SOD) and catalaz (CAT), and the level of glutathione (GSH) in all the tissues examined in lead-treated groups. As histopathological findings, the cellular damage induced by lead in the tissues of the kidney, liver and the heart were observed to have been partially prevented by PJ treatment. The protective effect of PJ was more pronounced in the testis compared to those others.
Effects of Sildenafil Citrate on the Hematological Parameters in the Early Phase of Wound Healing in Diabetic Rats
(Academic Journals inc, 2011) Tas, A.; Karasu, A.; Comba, B.; Aksu, D. S.; Duz, E.; Tanritanir, P.
In this study Investigation of effects of sildenafil citrate on some of the hematological parameters in the early phase of wound healing in diabetic rats was aimed in this study. Total 30 rats were used in the study. Rats were divided into three groups equal in number (control-non diabetic, diabetic and diabetic+Sc). While no treatment was given to the control-non diabetic and diabetic groups, sildenafil citrate was given to the diabetic+Sc group intraperitoneally with a dosage of 0.7 mg/kg/day dosage for 3 days after creating wounds: under ketamine anesthesia, a circular, full thickness, standard wound was created on the back of each rat by using a 0.8 cm sterile punch. Cardiac blood was drawn in tubes containing EDTA from each group before the creation of wounds and on days 1, 2 and 3 following the creation of wounds. Differential leukocyte counts were determined in blood samples containing anticoagulant. For this purpose, blood smears were prepared from blood samples and stained with Giemsa method. It was found that, while neutrophil counts increased in all the groups during the wound-healing periods with the increasing number of days, lymphocyte counts reduced and in control and Sc+diabetic groups, monocyte counts increased. As a result, it was concluded that Sc plays an important role in eliminating the pressure on the cellular activities in the early period of wound healing and continuance of the inflammatory process and wound healing within normal limits and also that this could be demonstrated with hematological analyses.