Browsing by Author "Aksu, Fazilet"

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The Effects of Mesenchymal Stem Cells Applied During the Subacute Period in Peripheral Neuropathy
(Cukurova Univ, Fac Medicine, 2024) Kiroglu, Olcay; Maytalman, Erkan; Alizade, Ares; Emre, Mustafa; Zorludemir, Suzan; Aksu, Fazilet
Purpose: The study aims to investigate the effect of bone marrow-derived mesenchymal stem cells (BM-MSCs) administered subacute period to neuropathic mice on allodynia and nerve-muscle tissue functions during 24 weeks. Materials and Methods: Peripheral neuropathy was induced by partial sciatic nerve ligation. Experiments were conducted in Control, Sham, Neuropathic, BM-MSC, and Neuropathic+BM-MSC groups. Allodynia was measured by cold plate test at the 2nd, nd , 6th, th , and 24th th weeks. Electrophysiological and histopathological examinations were performed on isolated nerve-muscle tissues at the end of the 24th th week. Results: Allodynia threshold increased in the Neuropathic+BM-MSC group (7.76 +/- 0.33 sec) from the 6 th week and continued to increase along 24 weeks compared to the Neuropathic group (4.36 +/- 0.21 sec). Action potential (137.9 +/- 7.85 mV) and depolarization (0.74 +/- 0.01 msec) values of the Neuropathic+BM-MSC group exhibited partial improvement compared to the Neuropathic group (121.5 +/- 3.03 mV and 0.81 +/- 0.02 msec, respectively) at the 24th th week. Muscle tissue's resting membrane potential values increased in the Neuropathic+BM-MSC group compared to the Neuropathic group (-73.4 +/- 0.2 and-87.7 +/- 0.2 mV, respectively). Histopathological examination of nerve tissue revealed loss of myelinated axons and significant fibrosis in the endoneurium in the Neuropathic group while Schwann cell proliferation and preservation of myelinated axons were observed in the Neuropathic+BMMSC group. Muscle fiber atrophy, compensatory hypertrophic fibers, and increased central nuclei were seen in the Neuropathic group, while small atrophic muscle fiber groups were identified in the Neuropathic+BM-MSC group. Conclusion: BM-MSC application in the subacute period is found to reduce allodynia and provide functional recovery in nerve-muscle tissue in experimental peripheral neuropathy.
In Vivo Assessment of a Capsaicin-Containing Microemulsion for Neuropathic Pain Management
(Galenos Publ House, 2025) Shayyah, Rumooz; Bolgen, Umay Merve Guven; Alizade, Ares; Kayiran, Serpil Demirci; Ebrahimi, Sonia; Aksu, Fazilet
BACKGROUND/AIMS: Capsaicin (CAP) has been used in clinical applications for the treatment of neuropathic pain (NP). The main disadvantages of CAPare itsshort half-life, lowwatersolubilityand low bioavailability. Thisstudy intended to producea therapeutically effective microemulsion (ME) formulation comprising CAP to decrease NP. MATERIALS AND METHODS: ME was prepared using oleic acid, Tween 80, propylene glycol, ethanol, and water. Neuropathy was induced by partial sciatic nerve ligation (PSL) in mice. Two weeks after, PSL NP was tested using the cold plate (CP) and von Frey (VF) tests. The mice in the treatment group were administered 10 mg/kg CAP by oral gavage. The effects on NP of conventional CAP and ME CAP were compared. RESULTS: The prepared ME formulation of (CAP) was a homogeneous, transparent, thermodynamically stable dispersion of water and oil. The classic CAP was not effective on NP, while ME CAP was effective in the CP. The ME CAP was more effective on mice, than classic CAP using the VF test. CONCLUSION: The developed novel ME formulation at lower doses could reduce side effects and improve the bioavailability of the oral administration of CAP in the treatment of NP, and thus, would achieve good patient compliance.
Investigation of Behavioral Changes and Histopathological Changes in the Brain in Alzheimer's Modeled Mice With Aluminium Chloride (AlCl3)
(Galenos Publ House, 2025) Kanan, Derya Deniz; Aydemir, Isil; Alizade, Ares; Ebrahimi, Sonia; Aksu, Fazilet
BACKGROUND/AIMS: Aluminium (Al) is related with many brain diseases including Alzheimer's disease, however the relation between Al and neurodegenerative diseases is still controversial. In this study behavioral and histopathological changes were investigated in the AlCl3 induced Alzheimer's model in mice. MATERIALS AND METHODS: Male and female mice divided into control (tap water) and test (50 mg/kg/day AlCl3) groups. After ninety days of AlCl3/water intake, rota rod, elevated plus maze, Passive Avoidance tests were performed to assess motor coordination, spatial and emotional learning and memory, respectively. After the behavioural experiments, histopathological examination was made in the brain. RESULTS: No difference was found between the groups and the genders in the rota-rot test. Learning and memory were impaired in both gender. Long term memory impaired female mice in the test group. Neuron loss was observed in both the cerebral cortex and hippocampus CA1 regions in the test group, while an increase in pycnotic nucleated cells was observed. Neurofibrillary tangles were also observed in the hippocampus, with neurons with basophilic nuclei prominently. CONCLUSION: As a result, long-term exposure to low doses of aluminum may cause behavioral changes and histopathological changes in the brain.