Browsing by Author "Aktar, Ahmet"
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Article The Effects of Supplementation of Vitamin D To the Egg-Yolk Extender on Cryopreservation of Ram Semen(Wiley, 2024) Aktar, Ahmet; Toker, Mehmed Berk; Koca, Davut; Uzun, Umit Can; Alcay, SelimObjectives: This study aimed to examine the effects of supplementation of vitamin D to the egg-yolk extender on characteristics of frozen-thawed ram semen. Methods: Semen samples obtained from adult rams were pooled and divided into five equal volumes. It was reconstituted with extenders containing different concentrations of vitamin D: 0 (control), 12.5 (VITD 12.5), 25 (VITD 25), 50 (VITD 50), and 100 ng/mL (VITD 100), and then they were frozen. Sperm motility parameters, plasma membrane functional integrity, acrosomal integrity, DNA fragmentation, and mitochondrial membrane potential of the groups were evaluated after sperm thawing. Results: Total motility and progressive motility were higher in VITD 50 than in all other groups (p < 0.05). Higher sperm straightness, linearity, and wooble were higher in VITD 50 than in the control group (p < 0.05). A similar pattern of VITD 50 was observed for plasma membrane integrity and mitochondrial membrane potential (p > 0.05). Conclusions: In the study, it was observed that adding vitamin D to the extender had a beneficial effect on ram spermatological parameters. In addition, it was concluded that the use of the 50 ng/mL vitamin D in the extender provided more effective protection than the other doses.Article Elecsys® Amh Assay: Determination of Anti-Müllerian Hormone Levels and Evaluation of the Relationship Between Superovulation Response in Holstein Dairy Cows(Wiley, 2024) Koca, Davut; Aktar, Ahmet; Turgut, Ali Osman; Sagirkaya, Hakan; Alcay, SelimBackground: Anti-Mullerian Hormone (AMH) serves as a crucial parameter in assessing the reproductive herd life and ovarian reserve in cattle. Consequently, extensive research is conducted on AMH levels. Various measurement methods can be employed to determine AMH levels. However, to our knowledge, no study has been conducted on Holstein donors using the Elecsys (R) AMH kit. Objective: This study was designed to determine AMH levels in donors utilising the Elecsys (R) AMH kit and to evaluate the relationship between superovulation response parameters and AMH levels. Methods In this study, we measured the serum AMH levels of 36 cows using the Elecsys (R) AMH automated assay before the superovulation protocol (1st sample) and FSH injections (2nd sample). The cows were categorised into three groups based on their AMH levels: low, medium, and high AMH. Results: Positive correlations were identified between AMH and parameters associated with superovulation response. The high AMH level group exhibited significantly greater numbers of corpus luteum, total embryos, transferable embryos, and grade 1 embryos compared to the medium and low AMH groups (p < 0.05) There was no significant difference between AMH levels before the superovulation protocol and FSH injections(p > 0.05). Body condition score and parity did not significantly affect AMH levels in cows (p > 0.05). Also, AMH cut-off values for the number of corpus luteum, total embryo, and transferable embryos were detected as 234, 227, and 210 pg/mL, respectively. Conclusion: These findings demonstrate that a high serum AMH level has a positive influence on the superovulation response. AMH can be used as a reliable marker for the selection of donors in Holstein cows.