Browsing by Author "Aktas, Abit"

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The Effect of Prenatal Exposure of a Non-Steroidal Anti-Inflammatory Drug on the Optic Nerve of Female Rats: a Stereological, Histological, and Electron Microscopic Study
(Taylor & Francis Ltd, 2013) Kaplan, Suleyman; Esrefoglu, Mukaddes; Aktas, Abit; Gul, Mehmet; Onger, Mehmet Emin; Altunkaynak, M. Eyup; Ragbetli, Murat Cetin
Objective: Non-steroidal anti-inflammatory drugs (NSAIDs) can have adverse effects for in both mother and fetus following administration during the prenatal period. If given during pregnancy, diclofenac sodium (DS), an NSAID, is given during the pregnancy, may also affect the development of the central nervous system (CNS) or related structures. Methods: Pregnant rats were separated into pure control (PG), saline (SG) and diclofenac groups (DG). A daily dose of 1 mg/kg of DS and 1 mL/kg saline was injected intraperitoneally to the DG and SG groups, respectively, from the 5th gestation day for a 15 day of period; the PG group received no treatment. After spontaneous delivery, female offspring were obtained from all groups. After the 20th week of postnatal life, the animals (n = 6 for each group) were perfused and the right optic nerves were resected. Sections were subjected to stereological and histological analysis. Results: There were no significant differences (p > 0.05) between PG, SG and DG groups with respect to myelin thickness, axonal cross-sectional area, axon numerical density, total section area of optic nerve and axon number. Conclusions: Histological and stereological results indicated that treatment with DS or saline produced undesirable effects on female rat optic nerve development and myelinization with respect to morphology.
Prenatal Exposure of Diclofenac Sodium Affects Morphology but Not Axon Number of the Median Nerve of Rats
(Termedia Publishing House Ltd, 2013) Ayranci, Ebru; Altunkaynak, Berrin Zuhal; Aktas, Abit; Ragbetli, Murat C.; Kaplan, Suleyman
The present study examined the effect of DS exposure on median nerve development in rats during prenatal life. Pregnant female rats were divided into three groups: a control group, a saline group and a DS group. Offspring of these animals were divided into 2 subgroups: 4 weeks old and 20 weeks old. Nerve samples were taken from the right legs and evaluated using stereological techniques in terms of the axon number, axon cross-sectional area, and myelin thickness. No drug-dependent macroscopic abnormality was observed in the nerve. No differences were noted for axon number in the control, saline, and DS groups of the same age and gender No gender difference was found for axon number or axon area between the other matched groups. In conclusion, prenatal exposure to diclofenac sodium does not affect axon number in rats, but can alter the morphology of the male and female median nerve.
Prenatal Exposure To a Non-Steroidal Anti-Inflammatory Drug or Saline Solution Impairs Sciatic Nerve Morphology: a Stereological and Histological Study
(Pergamon-elsevier Science Ltd, 2008) Canan, Sinan; Aktas, Abit; Ulkay, M. Basak; Colakoglu, Serdar; Ragbetli, Murat Cetin; Ayyildiz, Mustafa; Kaplan, Suleyman
The toxic effect of non-steroidal anti-inflammatory drugs (NSAIDs) during development has been widely investigated. While it has been shown that these drugs impair central nervous development and compromise the neural activity, the effects of these substances on the development of peripheral nerves are still not clarified. In the present Study, sciatic nerves withdrawn from three experimental groups of 4-week-old rats, prenatally exposed to either saline solution, or cliclofenac sodium, and controls not exposed to any substance, were evaluated in terms of axon number, cross-sectional area of axon and myelin sheet thickness as well as of the ultrastructure of nerve fibers. Comparisons of stereological estimations among these three groups showed that axon number and mean axon cross-sectional area, but not average myelin sheet thickness, were significantly decreased in rats that were exposed to both diclofenac sodium and also to the saline solution, in comparison of the control group. Electron microscope analysis revealed, in both treated groups, deterioration of myelin sheaths that was more pronounced in rats that were exposed to cliclofenac sodium. Altogether, these findings show that the prenatal administration of both diclofenac sodium and saline solution impairs peripheral nervous system development, thus suggesting that this potential teratogenic effect should be also taken into consideration in the clinical use of these substances in pregnant patients. (C) 2008 ISDN. Published by Elsevier Ltd. All rights reserved.