Browsing by Author "Aktas, Safiye"

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Parameters of Some of Biochemistry on Ischemia-Reperfusion Injury in Newborn Rat Intestine
(Asian Journal of Chemistry, 2010) Melek, Mehmet; Edirne, Yesim; Edirne, Tamer; Etensel, Barlas; Karaca, Irfan; Aktas, Safiye; Demir, Halit
The purpose of the present study was to determine the effects of treatment with thyroxin and dexamethasone separately and together on I/R injury in newborn rat intestine. Newborn rats were divided into five groups: (1) control (C) group, (2) sham group, (3) thyroxin (T(4)) group, (4) dexamethasone (DEX) group and (5) thyroxin plus dexamethasone (T(4)+DEX) group. Each group consisted of seven pups. Group T(4) received thyroxin I mu g/g BW/day, group DEX received dexamethasone 5 mu g/gBW/day and group T(4)+DEX received 1 mu g/gBW/day thyroxin and 5 mu g/gBW/day dexamethasone i.p. for 7 days. Group C received only physiological saline (NaCl 0.9%). Animals were sacrificed at the end of the reperfusion period and ileum samples were obtained. Malondialdehyde (MDA) levels, a product of lipid peroxidation, glutathione (GSH) levels, a key antioxidant were determined in ileum homogenates. In the jejunum, only T(4) caused a significant statistical elevation in GSH compared with control, sham and DEX groups (p < 0.05). There was significant statistical interaction between T(4) and DEX treatment, i.e. the effect of T(4) treatment was greater in both regions. Mucosal damage scores showed statistical significant effect of T(4) in the ileum compared with control and T(4)+DEX group. The same significant statistical effect was seen with T(4)+DEX group compared to the control group in the ileum. Overall, the comparison between the two regions shows a powerful effect of T(4) in both regions. There was statistical significance between the scores of T(4) in the ileum and of DEX and T(4)+DEX in the jejunum. A beneficial effect of thyroxin in all samples was observed in this study supporting its protective effects against I/R injury which was attenuated by glucocorticoid administration.
Role of N-Acetyl Cysteine and Acetyl-L Combination Treatment on Dna-Damage Genes Induced by Radiation in Hei-Oc1 Cells
(Taylor & Francis Ltd, 2018) Duzenli, Ufuk; Altun, Zekiye; Olgun, Yuksel; Aktas, Safiye; Pamukoglu, Ayca; Cetinayak, Hasan Oguz; Olgun, Levent
Purpose: The aim of the present study was to evaluate the effect of acetyl-l-carnitine (ALC) and N-acetyl cysteine (NAC) on ionizing radiation (IR)-induced cytotoxicity and change in DNA damage-related genes in House Ear Institute-Organ of Corti 1 (HEI-OC1) cells. Methods: HEI-OC1 cells were irradiated with 5 Gy radiation and treated by eight combinations of NAC and/or ALC: control, NAC, ALC, IR, NAC + IR, ALC + NAC, ALC + IR, and ALC + NAC + IR. Cell viability, apoptotic cell death, and DNA damage were measured at the 72nd hour. Eighty-four IR-induced DNA-damage-related genes were determined by RT-PCR gene array and >10-fold changes were considered significant. Results: IR decreased cell viability by about 50% at 72 hours of incubation. In particular, the ALC and/or NAC combination before IR protected the HEI-OC1 cells (p < .05). Single and combination treatment prior to IR led to lower apoptotic cell death (p < .05). There was a significant lower DNA damage in ALC + NAC + IR group compared to IR group (p < .05). Expressions of Brca2, Xpc, Mlh3, Rad51, Xrcc2, Hus1, Rad9a, Cdkn1a, Gadd45a which are the DNA-repair genes were found to be significantly higher in NAC + ALC + IR group than those in individual treatment of ALC or NAC. Conclusions: ALC and/or NAC treatment prior to IR led to higher cell viability and lower apoptotic cell damage compared to the IR group. The results of the study show that the ALC + NAC combination treatment inhibits DNA damage and induces DNA-repair genes to repair radiation damage, and this combination treatment is more effective against radiation-induced DNA damage than NAC or ALC therapy individually.