Browsing by Author "Akyildiz, G."

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First Detection of Ehrlichia Chaffeensis, Ehrlichia Canis, and Anaplasma Ovis in Rhipicephalus Bursa Ticks Collected From Sheep, Turkey
(Polska Akad Nauk, Polish Acad Sciences, Univ Warmia & Mazury Olsztyn, 2024) Ayan, A.; Celik, B. Aslan; Celik, O. Y.; Kilinc, O. Orunc; Akyildiz, G.; Yilmaz, A. B.; Babaoglu, A. R.
Anaplasmosis and ehrlichiosis are important tick -borne rickettsial diseases of medical and veterinary importance that cause economic losses in livestock. In this study, the prevalence of Anaplasma ovis, Ehrlichia canis and Ehrlichia chaffeensis was investigated in ticks collected from sheep in various farms in Van province, which is located in the Eastern Anatolian Region of Turkey. The ticks used in this study were collected by random sampling in 26 family farm business in 13 districts of Van province. A total of 688 ticks were collected from 88 sheep and 88 tick pools were created. All ticks identified morphologically as Rhipicephalus bursa. Phylogenetic analysis of Chaperonin and 16S rRNA gene sequences confirmed A. ovis, E. canis and E. chaffeensis in this study. Of the 88 tick pools tested, 28.41% (25/88) were positive for at least one pathogen. Anaplasma DNA was detected in five of the 88 pools (5.68%), E. canis DNA was detected in 19 of the 88 pools (21.59%), and E. chaffeensis DNA was detected in one of the 88 pools (1.14%) of R. bursa ticks. To our knowledge, this is the first report describing the presence of A. ovis, E. canis, and E. chaffeensis in R. bursa ticks collected from sheep in Turkey. Further studies are needed to investigate other co -infections in sheep in Turkey.
High Prevalence of Ehrlichia Canis in Dogs in Van, Turkey
(Aloki Applied Ecological Research and Forensic inst Ltd, 2020) Ayan, A.; Kilinc, Orunc O.; Erdogan, S.; Akyildiz, G.; Bia, M. M.; Lee, D.
Tropical and subtropical regions have seen an alarming increase in vector-borne diseases especially tick-borne diseases. Ehrlichiosis is one of the most important vector-borne disease from a zoonotic perspective and it can be even more lethal in dogs and humans a compromised immune system. This study was conducted to determine the prevalence and molecular characterization of E. canis in Van province of Turkey. A total of 387 blood samples were collected from dogs in Van veterinary clinics in 2019. Extracted DNAs were run through Nested PCR using the appropriate primers. A total of 79 samples out of 387 were E. canis positive at 389 bp revealed by Nested PCR. Sanger method was used for DNA sequencing of two selected positive samples. Phylogenetic analysis revealed that relevant amplicon was 100% compatible with 16S RNA gene isolated from E. canis in many geographical regions.
Occurence and Genotype Distribution of Cryptosporidium Spp., and Giardia Duodenalis in Sheep in Siirt, Turkey
(Polska Akad Nauk, Polish Acad Sciences, Univ Warmia & Mazury Olsztyn, 2023) Celik, B. Aslan; Celik, O. Y.; Ayan, A.; Kilinc, O. Orunc; Akyildiz, G.; Irak, K.; Ayan, O. Oktay
Cryptosporidium spp., and Giardia duodenalis are intestinal protozoan parasites known to infect humans and various animals and cause diarrhea. This study aimed at determining the prevalence and genotype of Cryptosporidium spp. and Giardia duodenalis in sheep in different locations of Siirt province. The fecal material for this study was collected from 500 sheep in different locations of Siirt province, Turkey. Fecal samples obtained from sheep were examined for Cryptosporidium spp. by Kinyoun Acid Fast staining and the Nested PCR method. Microscopic and Nested PCR methods revealed a prevalence of 2.4% (12/500) and 3.6% (18/500), respectively. Sequence analysis revealed the presence of C. ryanae, C. andersoni, and zoonotic C. parvum. In terms of Giardia duodenalis, 8.4% (42/500) and 10.2% (51/500) prevalence was determined using Nativ-Lugol and Nested PCR methods, respectively. Using sequence analysis, zoonotic assemblages A and B as well as assemblages E and D were detected. As a result of this study, both the prevalence of Cryptosporidium spp. and Giardia duodenalis and the presence of species that appear to be host-specific, as well as those known to be zoonotic, were revealed. A large-scale study is needed to understand the impact of these agents on sheep farming and their consequences on human health.