Browsing by Author "Algul, Sermin"

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Assessing the Effectiveness of Anaerobic Threshold and Respiratory Compensation Point on Fat and Carbohydrate Oxidations During Exercise in Sedentary Males
(Mattioli 1885, 2020) Ugras, Seda; Algul, Sermin; Ozcelik, Oguz
Study Objectives: Anaerobic threshold (AT) and respiratory compensation point (RCP) are two important metabolic set points. We aimed to determine the effects of exercise intensity at AT and RCP on the balance of substrate oxidation rates. Methods: Eleven male participants performed an incremental exercise test to exhaustion on a cycle ergometer to estimate AT and RCP. Subsequently, we conducted three 30 minute constant load exercise tests at AT (W-AT), RCP (W-RCP) and 25% below AT (W-
B7-H3: a Useful Emerging Diagnostic Marker for Colon Cancer
(Hindawi Ltd, 2022) Bostanci, Ozgur; Sayin, Pinar; Kiziltan, Remzi; Algul, Sermin; Aydin, Mehmet Akif; Kemik, Ozgur
Background. Colon cancer is the second most common malignancy causing the majority of cancer-related deaths. B7-H3 concentrations have drawn major interest as possible diagnostic biomarkers of cancer. The aim of this study was to measure the preoperative serum B7-H3 levels and to determine those that are replaced in colon cancer. Method. We measured preoperative serum B7-H3 concentrations of 90 patients aged 57-69 years diagnosed with colon cancer and 50 age-matched healthy individuals. B7-H3 levels were determined using the sandwich enzyme-linked immunosorbent assay (ELISA). Patients were categorized by stage based on the TNM staging system, and the serum levels of B7-H3 were compared between patients with different TNM stages. Result. No statistically significant difference was found between the patient and control groups in terms of age and gender. Preoperative serum B7-H3 levels were statistically significantly higher in patients with colon cancer than in the healthy group (p < 0.001). Preoperative serum B7-H3 concentration of the stage IV patients was significantly higher than those of the patients with stage I and stage II disease. In addition, higher serum B7-H3 levels were associated with lymph node status and distant metastasis in colon cancer. Conclusion. We showed that B7-H3 is highly expressed in colon cancer and can be used as a candidate diagnostic biomarker and a potential target in colon cancer in future.
Comparatively Evaluating the Effects of Exercising at the Anaerobic Threshold on Oxidative Stress and Serum Levels of Leptin, Nesfatin-1 and Irisin in Sedentary Male and Females
(Mattioli 1885, 2022) Ugras, Seda; Algul, Sermin; Ozdenk, Cagri
Study Objectives: Exercise has a great impact in increases of energy metabolism and imbalance of oxidant-antioxidant status. We aimed to analyse the association between exercise-induced oxidative stress and energy regulatory hormones of leptin, nesfatin-1 and irisin in sedentary male and female subjects. Methods:A total of 30 subjects (15 males and 15 females) performed an aerobic exercise approximately 45 min. Blood samples were taken at baseline and the end of the exercise. ELISA method was used to analyse parameters of leptin nesfatin-1, irisin, total oxidant status (TOS) and total antioxidant status (TAS). Result: Exercise caused a significant increase in levels of irisin and TOS but TAS level decreased. In addition, leptin and irisin levels increased significantly in females, but they did not change significantly in males. Conclusion: However, gender differences have a great impact on energy regulatory hormones which needed to be evaluated in further stud-ies. Energy regulatory hormones did not correlate with the change of TAS and TOS levels.
Comprehensive Review of Animal Models in Diabetes Research Using Chemical Agents
(Sage Publications inc, 2025) Algul, Sermin; Ozcelik, Oguz
Diabetes mellitus, characterized by insufficient insulin secretion and impaired insulin efficacy, disrupts carbohydrate, protein, and lipid metabolism. The global diabetic population is expected to double by 2025, from 380 million, posing a significant health challenge. Most diabetic individuals fall into the type 1 or type 2 categories, and diabetes adversely affects various organs, such as the kidneys, liver, nervous system, reproductive system, and eyes.This review focuses on animal models of diabetes induced by chemical agents, which are essential tools for understanding disease mechanisms, investigating complications, and testing antidiabetic drugs. Models include those caused by streptozotocin (STZ), alloxan, ferric nitrilotriacetate (Fe-NTA), dithizone, and anti-insulin serum.Streptozotocin (STZ)-induced diabetes models create type 1 and 2 diabetes by destroying pancreatic beta cells. The combination of STZ with nicotinamide mimics type 2 diabetes phenotypes. Alloxan induces a hyperglycemic state by causing free radical formation that selectively destroys pancreatic beta cells. Fe-NTA and dithizone also create diabetes models by damaging pancreatic beta cells. Anti-insulin serum models induce insulin resistance and hyperglycemia by generating antibodies against insulin receptors, leading to a condition similar to type 1 diabetes.Each model has unique characteristics that make it suitable for different aspects of diabetes research. These models are used to understand the pathogenesis of diabetes, develop new treatment strategies, and evaluate the efficacy of potential drugs. Le diab & egrave;te sucr & eacute;, caract & eacute;ris & eacute; par une s & eacute;cr & eacute;tion insuffisante d'insuline et une efficacit & eacute; r & eacute;duite de l'insuline, perturbe le m & eacute;tabolisme des glucides, des prot & eacute;ines et des lipides. La population mondiale actuelle de 380 millions de diab & eacute;tiques devrait doubler d'ici 2025, ce qui pose un probl & egrave;me de sant & eacute; important. La plupart des personnes diab & eacute;tiques entrent dans les cat & eacute;gories de type 1 ou de type 2. Le diab & egrave;te affecte n & eacute;gativement divers organes, tels que les reins, le foie, le syst & egrave;me nerveux, le syst & egrave;me reproducteur et les yeux.Cette revue se concentre sur les mod & egrave;les animaux de diab & egrave;te induit par des agents chimiques, qui sont des outils essentiels pour comprendre les m & eacute;canismes de la maladie, & eacute;tudier les complications et tester les m & eacute;dicaments antidiab & eacute;tiques. Les mod & egrave;les incluent ceux caus & eacute;s par la streptozotocine (STZ), l'alloxane, le nitrilotriac & eacute;tate ferrique (Fe-NTA), la dithizone et le s & eacute;rum anti-insuline.Les mod & egrave;les de diab & egrave;te induits par la streptozotocine (STZ) cr & eacute;ent un diab & egrave;te de type 1 et 2 en d & eacute;truisant les cellules b & ecirc;ta pancr & eacute;atiques. La combinaison de STZ et de nicotinamide imite les ph & eacute;notypes du diab & egrave;te de type 2. L'alloxane induit un & eacute;tat hyperglyc & eacute;mique en provoquant la formation de radicaux libres qui d & eacute;truisent s & eacute;lectivement les cellules b & ecirc;ta pancr & eacute;atiques. Le nitrilotriac & eacute;tate ferrique (Fe-NTA) et la dithizone cr & eacute;ent & eacute;galement des mod & egrave;les de diab & egrave;te en endommageant les cellules b & ecirc;ta pancr & eacute;atiques. Les mod & egrave;les s & eacute;riques anti-insuline induisent la r & eacute;sistance & agrave; l'insuline et l'hyperglyc & eacute;mie en g & eacute;n & eacute;rant des anticorps contre les r & eacute;cepteurs de l'insuline, conduisant ainsi & agrave; une condition similaire au diab & egrave;te de type 1.Chaque mod & egrave;le poss & egrave;de des caract & eacute;ristiques uniques qui le rendent adapt & eacute; & agrave; diff & eacute;rents aspects de la recherche sur le diab & egrave;te. Ces mod & egrave;les sont utilis & eacute;s pour comprendre la pathogen & egrave;se du diab & egrave;te, d & eacute;velopper de nouvelles strat & eacute;gies de traitement et & eacute;valuer l'efficacit & eacute; de m & eacute;dicaments potentiels. Der durch eine unzureichende Insulinsekretion und eine verminderte Insulinwirkung gekennzeichnete Diabetes mellitus bezeichnet eine St & ouml;rung des Kohlenhydrat-, Protein- und Fettstoffwechsels. Es wird erwartet, dass sich die Zahl der Diabetiker weltweit bis 2025 von 380 Millionen verdoppeln wird, was eine erhebliche gesundheitliche Herausforderung darstellt. Die meisten Diabetiker fallen in die Kategorien Typ 1 oder Typ 2, und die Krankheit beeintr & auml;chtigt verschiedene Organe wie Nieren, Leber, Nervensystem, Fortpflanzungssystem und Augen.Diese Untersuchung konzentriert sich auf Tiermodelle f & uuml;r durch chemische Substanzen ausgel & ouml;sten Diabetes, die ein wichtiges Instrument f & uuml;r das Verst & auml;ndnis der Krankheitsmechanismen, die Untersuchung von Komplikationen und die Pr & uuml;fung von Antidiabetika sind. Zu den Modellen geh & ouml;ren solche, die durch Streptozotocin (STZ), Alloxan, Eisen-Nitrilotriacetat (Fe-NTA), Dithizon und Anti-Insulin-Serum verursacht werden.Streptozotocin (STZ)-induzierte Diabetesmodelle erzeugen Typ-1- und Typ-2-Diabetes durch Zerst & ouml;rung der Betazellen der Bauchspeicheldr & uuml;se. Die Kombination von STZ mit Nicotinamid ahmt den Ph & auml;notyp des Typ-2-Diabetes nach. Alloxan l & ouml;st einen hyperglyk & auml;mischen Zustand durch die Bildung freier Radikale aus, die selektiv die Betazellen der Bauchspeicheldr & uuml;se zerst & ouml;ren. Nitrilotriacetat aus Eisen (Fe-NTA) und Dithizon erzeugen ebenfalls Diabetesmodelle, indem sie die Betazellen der Bauchspeicheldr & uuml;se sch & auml;digen. Anti-Insulin-Serum-Modelle induzieren Insulinresistenz und Hyperglyk & auml;mie durch die Bildung von Antik & ouml;rpern gegen Insulinrezeptoren, was zu einer dem Typ-1-Diabetes & auml;hnlichen Erkrankung f & uuml;hrt.Jedes Modell hat spezielle Charakteristiken, die es f & uuml;r verschiedene Aspekte der Diabetesforschung qualifizieren. Diese Modelle dienen dazu, Aufschluss & uuml;ber die Entstehung von Diabetes zu gewinnen, neue Behandlungsstrategien zu entwickeln und die Wirksamkeit potenzieller Medikamente zu bewerten. La diabetes mellitus, que se caracteriza por una secreci & oacute;n insuficiente de insulina y un deterioro de su eficacia, altera el metabolismo de los hidratos de carbono, las prote & iacute;nas y los l & iacute;pidos. La poblaci & oacute;n diab & eacute;tica mundial se espera que se duplique, superando los 380 millones para el a & ntilde;o 2025, lo que plantea un desaf & iacute;o significativo para la salud. La mayor & iacute;a de las personas con diabetes pertenecen a las categor & iacute;as de tipo 1 o tipo 2, y la diabetes afecta negativamente a varios & oacute;rganos, como los ri & ntilde;ones, el h & iacute;gado, el sistema nervioso, el sistema reproductivo y los ojos.Este estudio se centra en los modelos de animales con diabetes inducida por agentes qu & iacute;micos, que son herramientas esenciales para comprender los mecanismos de la enfermedad, investigar las complicaciones y probar los f & aacute;rmacos antidiab & eacute;ticos. Los modelos incluyen los provocados por la estreptozotocina (STZ), el aloxano, el nitrilotriacetato f & eacute;rrico (Fe-NTA), la ditizona y el suero antiinsul & iacute;nico.Los modelos con diabetes inducida por estreptozotocina (STZ) crean diabetes de tipo 1 y 2 al destruir las c & eacute;lulas beta pancre & aacute;ticas. La combinaci & oacute;n de STZ con nicotinamida imita los fenotipos de la diabetes de tipo 2. El aloxano induce un estado hipergluc & eacute;mico al provocar la formaci & oacute;n de radicales libres que destruyen selectivamente las c & eacute;lulas beta pancre & aacute;ticas. El nitrilotriacetato f & eacute;rrico (Fe-NTA) y la ditizona tambi & eacute;n generan modelos con diabetes al da & ntilde;ar las c & eacute;lulas beta pancre & aacute;ticas. Los modelos con suero antiinsulina inducen resistencia a la insulina e hiperglucemia al generar anticuerpos contra receptores de insulina, lo que conduce a una afecci & oacute;n similar a la diabetes tipo 1.Cada modelo tiene caracter & iacute;sticas & uacute;nicas que lo hacen adecuado para diferentes aspectos de la investigaci & oacute;n sobre la diabetes. Estos modelos se utilizan para comprender la patog & eacute;nesis de la diabetes, desarrollar nuevas estrategias de tratamiento y evaluar la eficacia de posibles f & aacute;rmacos.
The Correlation Between the Differences in Nucb2/Nesfatin(nes) Peptide Levels and Body Weight, Lenght and Gender in Alburnus Tarichi
(Central Fisheries Research inst, 2018) Caf, Fatma; Koprucu, Sibel; Algul, Sermin; Koyun, Mustafa; Atici, Ataman Altug
Nucleobindin-2 (NUCB2) is a 396-amino-acid peptide related to food intake and body weight. It suppresses appetite by acting on the hypothalamus, andits derived peptide, nesfatin-1, was shown to reduce food intake and body weight in rodents. In this study, we examined NUCB2 peptide levels in correlation with body weight, length and gender in adult Alburnus tarichi fish. Serum NUCB2 levels were measured with ELISA, and were observed to significantly vary depending on gender: 0.43 +/- 0.17 ng/mL in the males (n=6) and 0.28 +/- 0.11 ng/ml in the females (n= 6) (p<0.05). However, NUCB2 levels were not significantly correlated to body weight (r=-0.08, p=0.7) or length (r=-0.16, p= 0.6). NUCB2/nesfatin, an appetiteregulation hormone, was therefore shown to be impacted by gender but not by body weight or length in A. tarichi.
Determination of Substrate Utilisation Rate and Amounts During Low and Moderate Intensity Constant Load Exercise Test
(Wiley, 2017) Algul, Sermin; Ozcelik, Oguz
Determination of the Relationships Between Anaerobic Threshold and O2 Pulse During Constant Load and Incremental Exercise Tests
(Wiley, 2017) Ozcelik, Oguz; Algul, Sermin
Effects of 3.5 Ghz Radiofrequency Radiation on Ghrelin, Nesfatin-1, and Irisin Level in Diabetic and Healthy Brains
(Elsevier, 2022) Bektas, Hava; Algul, Sermin; Altindag, Fikret; Yegin, Korkut; Akdag, Mehmet Zulkuf; Dasdag, Suleyman
Diabetes, mobile phone use, and obesity have increased simultaneously in recent years. The radiofrequency radiation (RFR) emitted from mobile phones is largely absorbed in the heads of users. With 5 G, which has started to be used in some countries without the necessary precautions being taken, the amount of RFR to which living things are exposed will increase. In this study, the changes in energy homeostasis and redox balance caused by 5 G (3.5 GHz, GSM-modulated) were explored. The effects of RFR on the brains of diabetic and healthy rats were investigated and histopathological analysis was performed. Twenty-eight Wistar albino rats weighing 200-250 g were divided into 4 groups as sham, RFR, diabetes, and RFR+diabetes groups (n = 7). The rats in each group were kept in a plexiglass carousel for 2 h a day for 30 days. While the rats in the experimental groups were exposed to RFR for 2 h a day, the rats in the sham group were kept under the same experimental conditions but with the radiofrequency generator turned off. At the end of the experiment, brain tissues were collected from euthanized rats. Total antioxidant (TAS), total oxidant (TOS), hydrogen peroxide (H2O2), ghrelin, nesfatin-1, and irisin levels were determined. In addition, histopathological analyses of the brain tissues were performed. The specific absorption rate in the gray matter of the brain was calculated as 323 mW/kg and 195 mW/kg for 1 g and 10 g averaging, respectively. After RFR exposure among diabetic and healthy rats, decreased TAS levels and increased TOS and H2O2 levels were observed in brain tissues. RFR caused increases in ghrelin and irisin and a decrease in nesfatin-1 in the brain. It was also observed that RFR increased the number of degenerated neurons in the hippocampus. Our results indicate that 3.5 GHz RFR causes changes in the energy metabolism and appetite of both healthy and diabetic rats. Thus, 5 G may not be innocent in terms of its biological effects, especially in the presence of diabetes.
Effects of 3.5-Ghz Radiofrequency Radiation on Energy-Regulatory Hormone Levels in the Blood and Adipose Tissue
(Wiley, 2024) Bektas, Hava; Dasdag, Suleyman; Altindag, Fikret; Akdag, Mehmet Z.; Yegin, Korkut; Algul, Sermin
In recent years exposure of living beings to radiofrequency radiation (RFR) emitted from wireless equipment has increased. In this study, we investigated the effects of 3.5-GHz RFR on hormones that regulate energy metabolism in the body. Twenty-eight rats were divided into four groups: healthy sham (n = 7), healthy RFR (n = 7), diabetic sham (n = 7), and diabetic RFR (n = 7). Over a month, each group spent 2 h/day in a Plexiglas carousel. The rats in the experimental group were exposed to RFR, but the sham groups were not. At the end of the experiment, blood and adipose tissues were collected from euthanized rats. Total antioxidant, total oxidant, hydrogen peroxide, ghrelin, nesfatin-1, and irisin were determined. Insulin expression in pancreatic tissues was examined by immunohistochemical analysis. Whole body specific absorption rate was 37 mW/kg. For the parameters analyzed in blood and fat, the estimated effect size varied within the ranges of 0.215-0.929 and 0.503-0.839, respectively. The blood and adipose nesfatin-1 (p = 0.002), blood and pancreatic insulin are decreased, (p = 0.001), gherelin (p = 0.020), irisin (p = 0.020), and blood glucose (p = 0.040) are increased in healthy and diabetic rats exposed to RFR. While nesfatin-1 are negatively correlated with oxidative stress, hyperglycemia and insulin, ghrelin and irisin are positively correlated with oxidative stress and hyperglycemia. Thus, RFR may have deleterious effects on energy metabolism, particularly in the presence of diabetes. 3.5 GHz radiofrequency radiation (RFR) may induce alterations in hormones regulating energy metabolism. 3.5 GHz RFR may lead to alterations in total antioxidant, total oxidant, and hydrogen peroxide levels. Particularly in conjunction with diabetes, 3.5 GHz RFR may result in adverse effects on energy metabolism. Although there were changes of the hormone levels in the exposed group, the actual values remained for both sham and exposed groups within the normal range.
Effects of Curcumin on Sperm Quality, Lipid Profile, Antioxidant Activity and Histopathological Changes in Streptozotocin-Induced Diabetes in Rats
(Wiley, 2020) Belhan, Saadet; Yildirim, Serkan; Huyut, Zubeyir; Ozdek, Ugur; Oto, Gokhan; Algul, Sermin
In this study, the effect of low-dose curcumin on sperm parameters, reproductive hormones, lipid profile, biochemical antioxidant parameters and the histopathological structure of the testis in diabetic male rats were evaluated. In the study, 28 male Wistar albino rats weighing 300-370 g and aged 8-10 weeks were used. Four groups of equal numbers have been created. Diabetes mellitus was induced with 45 mg/kg streptozotocin (STZ) in seven rats. Curcumin was administered to the rats in curcumin and the diabetes + curcumin group by gavage for 15 days at a dose of 10 mg/kg. Then, the rats were sacrificed. Blood samples and testis tissues were obtained, while the rats were under anaesthesia. Glucose, lipid profile, reproductive hormones, sperm parameters, biochemical antioxidant parameters and histopathological examination of the testis were performed. Abnormal sperm ratio, malondialdehyde, glucose, cholesterol, low-density lipoprotein, and triglyceride levels and caspase-3 expression were increased in diabetic rats, while the sperm motility and intensity and reduced glutathione, catalase and testosterone levels were decreased. When low-dose curcumin (10 mg/kg) was administered to diabetic rats, we found that curcumin significantly increased sperm motility and density, and decreased abnormal sperm rate according to the diabetic group. Moreover, curcumin significantly suppressed the lipid profile and increased follicle-stimulating hormone (FSH) and testosterone levels compared to the diabetic group. On testicular damage and decreased reproductive hormones caused by diabetes, curcumin may have a protective effect with indirect effect of glycaemic control by curcumin.
Effects of Morning and Nocturnal Soccer Matches on Levels of Some Trace Elements in Young Trained Males
(C M B Assoc, 2019) Algul, Sermin; Bengu, Aydin Sukru; Baltaci, Saltuk Bugra; Ozcelik, Oguz
The aim of this study was to comparatively evaluate effect of morning and nocturnal soccer matches induced metabolic stress on plasma levels of iron (Fe), copper (Cu) and zinc (Zn). Twenty male footballers performed two soccer matches in morning and at night on different days. Blood samples were taken before and tiller match. The levels of Fe, Zn and Cu were measured through an atomic absorption spectrophotometry. Metabolic stress was evaluated by altered malondialdehyde (MDA) levels that measured using High Performance Liquid Chromatography. In morning and at nocturnal soccer matches, levels of MDA (36% and 27%), Fe (37.4% and 38.9%) and Cu (34.8% and 26.8%) were all increased in all subjects, respectively. However, Zn level decreased -4.5 % in morning (n=10 subjects) and -9.4% at nocturnal (n=12 subjects) soccer matches. In addition, Cu/Zn ratio increased significantly 46.6% in morning and 36.6% at nocturnal soccer matches. Soccer match has significant effects on levels of MDA, Fe and Cu but not Zn levels. The results of this study showed that morning soccer match significantly alters levels of MDA and Cu and Cu/Zn ratio compared to nocturnal soccer match.
Effects of Training Status on Ventilatory Efficiency During Incremental Exercise Test
(Wiley, 2017) Ozdenk, Cagri.; Algul, Sermin; Ozcelik, Oguz
Epcam Is a New Potential Serum Biomarker for Early Gastric Cancer
(Bayrakol Medical Publisher, 2023) Eroglu, Ersan; Kiziltan, Remzi; Algul, Sermin; Kemik, Ozgur; Altinli, Ediz
Aim: Overexpression of epithelial cell adhesion molecule (EpCAM) has been shown in a wide spectrum of epithelial cancers. In this study we aimed to examine the utility of serum EpCAM levels as a new marker for early diagnosis of gastric cancer, and to determine its association with different stages of gastric cancer. Material and Methods: A total of 88 patients who underwent resection due to gastric cancer and 44 healthy subjects as the control group were included in the study. The diagnosis of gastric cancer was confirmed by a combination of upper gastrointestinal endoscopy, endoscopic ultrasonography, and histopathology. EpCAM levels were evaluated using the enzyme-linked immunosorbent assay (ELISA) method following the instructions of the manufacturer. Results: The mean preoperative EpCAM level was found as 235.7 +/- 34.2 pg/mL in the patient group and 42.5 +/- 2.38 pg/mL in the control group. The mean preoperative serum EpCAM level was found to be significantly higher in gastric cancer patients compared to the control group (p<0.001). In addition, mean EpCAM levels were higher in patients with class T4 and T2 compared to T1. Mean EpCAM levels were also significantly higher in N1 and N2 classes compared to N0, and in M1 compared to M0 (all, p<0.001). Discussion: The results of this study indicate that the elevated expression of the EpCAM levels can predispose to the development of gastric cancer. Thus, the analysis of this marker could be a useful biomarker for screening patients with gastric cancer.
Evaluating the Energy Regulatory Hormones of Nesfatin-1, Irisin, Adropin and Preptin in Multiple Sclerosis
(Elsevier Sci Ltd, 2022) Algul, Sermin; Ozcelik, Oguz
Background: Nesfatin-1, irisin, adropin and preptin were originally introduced as energy regulatory hormones. However, the results of studies revealed that these hormones may also have important roles in inflammation, immune function and neurological impairment. Multiple sclerosis (MS) is a chronic autoimmune disease, characterized by progressive inflammation, demyelination, and axonal loss in the central nervous system. We aimed to evaluate nesfatin-1, irisin, adropin and preptin hormones in patients with MS accompanied by inflammation and central nervous system dysfunction.Materials and methods: A total of 110 subjects (65 patients with relapsing-remitting MS and 45 healthy individuals as control group) were included in this study. Venous blood samples were collected between 7:30 a.m. and 9:00 a.m. Serum concentrations of all markers were measured by enzyme linked immunoassay methods. The unpaired t-test was used to investigate between-group differences.Results: The nesfatin-1, irisin, adropin and preptin levels were found to be significantly lower in the MS group compared to the control group (p < 0.05).Conclusion: In the present study, circulating nesfatin-1, irisin, adropin and preptin levels were decreased in patients with MS. However, the pathogenesis of MS and the underlying molecular mechanism of these hormones in MS have still not been elucidated. Further investigations with larger sample sizes and longer periods are required to obtain satisfactory information. In conclusion, the energy regulatory hormones of nesfatin-1, irisin, adropin and preptin may have potential for the development of new therapeutic targets for treatment of inflammatory diseases.
Evaluating the Impact of Metabolic and Cognitive Stress on Ghrelin and Nesfatin-1 Hormones in Patients With Diabetes and Diabetic Depression
(Wiley, 2024) Algul, Sermin; Ozcelik, Oguz
Nesfatin-1 and ghrelin, initially recognised as hormones involved in regulating energy, have emerged as crucial players with vital functions in various human body systems. In this study, we conducted a comparative assessment of nesfatin-1 and ghrelin responses in individuals experiencing metabolic stress due to diabetes, those with depressive diabetes characterised by both metabolic and mental stress, and healthy controls. We collected blood samples from a total of 90 participants, consisting of 30 people with type II diabetes mellitus (DM), 30 people with type II DM and major depressive disorders, and 30 healthy individuals. Diabetes was diagnosed based on glycated haemoglobin (HbA1c) levels, while depression was assessed using DSM-V criteria. Insulin resistance (HOMA-IR) was calculated, and serum ghrelin and nesfatin-1 levels were measured using ELISA kits. We observed statistically significant decreases in nesfatin-1 and ghrelin levels in the diabetic group (p < 0.0001). However, in the depressive diabetic group, nesfatin-1 levels increased significantly, while ghrelin levels decreased further. The nesfatin-1 to ghrelin ratio decreased in the diabetic group but increased significantly in the depressive diabetic group (p < 0.0001). Nesfatin-1 and ghrelin hormones exhibit parallel impacts in response to metabolic stress, but nesfatin-1 demonstrates contrasting actions compared to ghrelin when mental stress is added to metabolic stress. The findings of this study suggest that nesfatin-1 and ghrelin hormones may play active roles as protective, prognostic, and even etiological factors in various stress situations, particularly those involving mental stress, in addition to their known functions in regulating energy.
Evaluating the Levels of Nesfatin-1 and Ghrelin Hormones in Patients With Moderate and Severe Major Depressive Disorders
(Korean Neuropsychiatric Assoc, 2018) Algul, Sermin; Ozcelik, Oguz
Objective The goal of this study was to evaluate the importance of nesfatin-1, acylated and des-acylated ghrelin, which are known as energy regulatory hormones, in patients with moderate and severe major depression disorders (MDD). Methods Thirty patients with a moderate degree of MDD and, 30 with a severe degree of MDD were used as participants in this study. Thirty subjects without depression were enrolled as a control group. The Hamilton Depression Rating Scale was used to classify the patients with MDD. Blood samples were taken after overnight fasting. The plasma nesfatin-1, acylated ghrelin and des-acylated ghrelin levels were measured using a commercially available enzyme-linked immunosorbent assay kit. Results The nesfatin-1, the acylated ghrelin and the des-acylated ghrelin levels were found to be significantly higher in severe MDD (3.92 +/- 0.4 ng/mL; 88.56 +/- 4.1 pg/mL; 962.76 +/- 67 pg/mL) as compared to moderate MDD (2.91 +/- 0.5 ng/mL; 77.63 +/- 4.19 pg/mL; 631.16 +/- 35 pg/mL), or the control (1.01 +/- 0.3 ng/mL; 58.60 +/- 9.00 pg/mL; 543.13 +/- 62 pg/mL), respectively. Conclusion Although nesfatin-1 and ghrelin are known as adversely affecting the hormones involving the regulation of appetite and food intake, they all increase in depressive patients and are even associated with the severity of the disease. In clinical medicine, the evaluation of the role of nesfatin-1 and ghrelin in endocrine and neu-roendocrine regulation of major metabolic functions is an important key mechanism in solving numerous diseases associated with endocrine and neuroendocrine disturbance. Increased levels of nesfatin-1 and ghrelin may also be important criteria in describing the prognoses of the patients and the effectiveness of the treatments.
Evaluation of Aerobic Exercise Induced Metabolic Stress on Serum Asprosin Levels: Comparison of Fitness Status
(Mattioli 1885, 2021) Algul, Sermin; Deniz, Gulnihal; Alp, Hamit Hakan; Ugras, Seda; Ozcelik, Oguz
We comparatively evaluated effects of aerobic exercise performed morning and afternoon on asprosin levels in young trained, sedentary males. A total of fifty male subjects (trained n=25, sedentary n=25) participated in morning and afternoon aerobic running exercises to approximately 70% of the subject's maximal heart rate for about 45 min. Pre- and post-exercise venous blood samples were taken and analysed for asprosin using ELISA. Serum CK and MDA levels were determined by measuring with an autoanalysers and a HPLC, respectively. Asprosin, CK and MDA levels increased significantly at the end of both morning (p<0.05), and afternoon (p<0.05) exercises in the trained and sedentary group. At the end of the acute running exercise in the trained group in the morning, the asprosine, MDA and CK levels increased by 24%, 29% and 32%; while they increased by 24%, 30%, and 40% in the afternoon, respectively. In the sedentary group, asprosin, MDA and CK levels increased by 31%, 38% and 31% after the acute running exercise in the morning, while the asprosin, MDA and CK levels increased by 34%, 58% and 42% after the acute running exercise in the afternoon, respectively. Asprosin levels increased in all subjects in both aerobic exercise tests without correlating increase of MDA and CK. Altered asprosin levels could be related other factors rather than metabolic and muscular stress parameters.
Evaluation of Ghrelin, Nesfatin-1 and Irisin Levels of Serum and Brain After Acute or Chronic Pentylenetetrazole Administrations in Rats Using Sodium Valproate
(Taylor & Francis Ltd, 2018) Erkec, Ozlem Ergul; Algul, Sermin; Kara, Mehmet
Objectives: In this study, we aim to reveal the alterations (due to seizure) in the serum and brain levels of nesfatin-1, ghrelin and irisin after acute or chronic pentylenetetrazole administrations in rats using sodium valproate. Methods: 35 Wistar albino rats were randomly divided into five groups: Control, Acute Pentylenetetrazole group (APTZ), Acute Pentylenetetrazole+ Valproate group (AVPA), PTZ kindling group (PTZk) and PTZ kindling+ Valproate group (KVPA). Serum and brain levels of ghrelin, nesfatin-1 and FNDC5/irisin were determined with ELISA. Results: Serum levels of ghrelin were significantly decreased in APTZ and PTZk groups compared to the control (p < 0.01). There was a statistically significant decrease in brain levels of ghrelin in all groups compared to the control group (p < 0.01). There was a statistically significant increase in serum nesfatin-1 levels in the APTZ and PTZk groups compared to the control (p < 0.05). Serum levels of nesfatin-1 were similar to the control group in both the acute and the chronic treatment groups. There was a statistically significant increase in brain nesfatin-1 levels of the KVPA group compared to the control (p < 0.05). Serum and brain levels of FNDC5/irisin were found significantly increased in APTZ, AVPA and PTZk groups compared to the control (p < 0.01). Conclusions: Statistically significant alterations were detected in the serum and brain levels of these three peptides in both the PTZ-induced chronic epilepsy model and acute seizure model. The results of this study may suggest that the increase in FNDC5/irisin and nesfatin-1 levels, and the decrease in ghrelin levels may contribute to seizure pathophysiology. However, further studies are needed in order to confirm our hypothesis.
Evaluation of Total Antioxidant Status, Total Oxidant Status and Oxidative Stress Index of Some Economically Important Plants From Turkey
(Mattioli 1885, 2018) Caf, Fatma; Kilic, Omer; Algul, Sermin
This study aims to develop antioxidant properties of thirty four economically important plants of Asteraceae and Lamiaceae families, from Turkey. Analyses were done with reliable measurement research kits. Total antioxidant status, total oxidant status were determined with a novel method using available enzyme-linked immuno assay kit (TOS Cat. No: RL0024, TAS Cat. No: RL0017). Total antioxidant (TAS) level of studied Aster aceae and Lamiaceae taxa were detected as 71.61 +/- 37.05 mmol/L and 36.97 +/- 9.6 mmol/L, respectively. In addition, TAS level of Asteraceae taxa was found to be higher (p<0.38) than Lamiaceae taxa. When compared total oxidant (TOS) level of Asteraceae (3.02 +/- 0.66) and Lamiaceae taxa (5.26 +/- 1.06), no significant (p=0.08) differences were observed. In the similar way OSI levels in the Asteraceae (12.52 +/- 3.63 AU) Lamiaceae families (30.8 +/- 8.4 AU) were found to be no significant difference (p=0.05). The present study concluded that antioxidative feature of studied plant taxa is remarkable. So studied plant taxa are emerges a natural source of antioxidants and could be a good experimental system for further researches.
Individual Variation of Leptin, Nesfatin-1 and Irisin Levels During Aerobic Exercise Performed Morning and Night in Trained and Untrained Male Subjects
(Wiley-blackwell, 2016) Algul, Sermin; Ozcelik, Oguz