Browsing by Author "Alisarli, Mustafa"

Now showing 1 - 7 of 7

Classical Enterotoxigenic Characteristics of Staphylococcus Aureus Strains Isolated From Bovine Subclinical Mastitis in Van, Turkey
(Elsevier, 2008) Boynukara, Banur; Gulhan, Timur; Alisarli, Mustafa; Gurturk, Kemal; Solmaz, Hasan
The aim of the present study was to investigate classical enterotoxigenic properties of Staphylococcus aureus strains isolated from cows with subclinical mastitis. For this purpose, 480 milk samples from cows with subclinical mastitis raised in different villages neighbouring Van city Center were collected. A total of 106 S. aureus strains were isolated. Twenty seven isolates (25.5%) were found to be enterotoxigenic by reverse passive latex agglutination (RPLA). Of these, 25 (23.6%) were positive for staphylococcal enterotoxin A (SEA), 2 (1.9%) for SEB. None of the isolates was positive for SEC or SED. This study showed that most S. aureus strains isolated from bovine subclinical mastitis produced SEA compared to other SEs. (C) 2008 Elsevier B.V. All rights reserved.
Evolution of Slime Production by Coagulase-Negative Staphylococci and Enterotoxigenic Characteristics of Staphylococcus Aureus Strains Isolated From Various Human Clinical Specimens
(Soc General Microbiology, 2007) Boynukara, Banur; Gulhan, Timur; Gurturk, Kemal; Alisarli, Mustafa; Ogun, Erdal
The present study was designed to determine the slime production of coag u lase- negative staphylococci (CoNS) and the enterotoxigenic properties of Staphylococcus aureus strains, and to evaluate the clinical importance of slime-producing CoNS and enterotoxigenic S. aureus strains isolated from various human clinical specimens. For this purpose, a total of 120 Staphylococcus strains were isolated and identified, and further characterized for their slime production and enterotoxigenicity. Of the clinical isolates, 55 (45.8 %) were found to be S. aureus, and the others (54.2 %) were identified as CoNS. Of the CoNS, 20 (116.7 %) were further identified as Staphylococcus hominis, 18 (15 %) as Staphylococcus epidermidis, six (5 %) as Staphylococcus xylosus, six (5 %) as Staphylococcus warneri, five (4.2 as Staphylococcus sciuri, four (3.3 %) as Staphylococcus haemolyticus, and two each (1. 7 as Staphylococcus simulans, Staphylococcus capitis and Staphylococcus saprophyticus, respectively. Thirty-nine (60%) of 65 CoNS were found to be slime producers. Slime production was observed in all CoNS, except S. capitis, mostly from blood (38.5 %), tracheal aspiration (20.5 %) and urine (112.8 %) specimens. In addition, of the 55 S. aureus isolates, 46 (83.6 %) were found to be enterotoxigenic, and of these S. aureus strains, 39 (84.7 %) were positive for staphylococcal enterotoxin (SE)A. The results of this study showed that the slime-producing CoNS were mostly found in clinical specimens of blood, tracheal aspirate and urine. SEA was the predominant enterotoxin type detected in S. aureus strains from human clinical specimens.
Fluoride Levels of Drinking Water in Bitlis Province (Turkey)
(Kafkas Univ, veteriner Fakultesi dergisi, 2011) Kahraman, Tahir; Alemdar, Suleyman; Alisarli, Mustafa; Agaoglu, Sema
In the present study, fluoride levels of drinking water from the province of Bitlis and its towns (Adilcevaz, Ahlat, Guroymak, Hizan and Tatvan) were investigated. A total of 164 water samples, collected from tanks or tap in autumn (November 2006) and spring (May 2007) were used as material. The fluoride levels were measured by Orion model 720A potentiometer, using an ion-selective electrode. The fluoride values in drinking water samples obtained from tanks and tap were found to be 0.36 +/- 0.05 and 0.35 +/- 0.03 ppm respectively. These values were the highest in Ahlat (0.75 +/- 0.04 ppm), and the lowest in the center of Bitlis (0.01 +/- 0.01 ppm). Fluoride levels were determined below 0.50 ppm in the 62% of water samples, and below 1.50 ppm in all water samples. The effect of season on the fluoride levels were found to be statistically insignificant (P > 0.05), while the effect of residential areas were significant (P < 0.05). As a result, it was demonstrated that the fluoride levels investigated in all water samples were within standard ranges, which poses no risk for fluoride toxication in the province of Bitlis.
Nitrate and Nitrite Levels of Drinking Water in Bitlis Province, Turkey
(Medwell online, 2009) Alemdar, Suleyman; Kahraman, Tahir; Agaoglu, Sema; Alisarli, Mustafa
In this study, nitrate and nitrite levels of drinking water in city of Bitlis were investigated. A total of 164 water samples, collected from the tank and tap water in Spring and Autumn were used as material. Analysis performed through spectrometric techniques showed that all samples contained some levels of nitrate and nitrite. The average nitrate and nitrite levels were 5.68+/-0.31 and 1.32+/-0.09 mg L-1, respectively. The effects of residential areas and season on the nitrate and nitrite levels were found to be statistically significant (p<0.05). Nitrate levels of tank and tap water were in accordance with recommended standards. However, 12 and 100% of tank water samples and 7.02 and 100% of tap water samples collected respectively in Spring and Autumn did not meet the national standards for nitrite levels. In addition, 3.58% of tap water collected in Autumn did not meet the international standards.
Prevalence of Verocytotoxigenic Escherichia Coli O157:h7 on Chicken Carcasses Sold in Turkey
(Natl veterinary Research inst, 2006) Akkaya, Levent; Atabay, Halil Ibrahim; Kenar, Beytullah; Alisarli, Mustafa
The prevalence of Escherichia coli O157:117 was investigated on samples of various portions of 190 chicken carcasses, obtained from local retail markets and poultry shops in Turkey. Immunomagnetic separation methods Was used to isolate E. coli O157:H7 from the samples. Verotoxin 1 (VT1) and verotoxin 2 (VT2) productions by the isolated E. coli strains was also investigated. E. coli O157:H7 was isolated from two (1.05%) of the 190 samples of poultry meat examined. All the strains isolated produced both VT1 and VT2. These findings indicate that poultry meat can also be a source of E. coli O157:H7 infections for humans. Appropriate control measures should. be developed and implemented to eliminate this human pathogen front foods of animal origin, including poultry, in order to protect human health.
Some Microbiological and Physicochemical Properties of Drinking Water in Bitlis District
(Foundation Environmental Protection & Research-fepr, 2009) Alemdar, Sueleyman; Kahraman, Tahir; Agaoglu, Sema; Alisarli, Mustafa
In the present study, microbiological and physicochemical qualities of drinking water obtained from the city of Bitlis and its provinces Adilcevaz, Ahlat, Guroymak, Hizan and Tatvan) were studied. A total of 164 water samples, collected from tank and tap waters in spring and autumn, were used as material. Aerobic plate counts were carried out by pour plate technique. While membrane filtration method was used for the counts of enterococcus, co iform, E. coli and sulphite reducing anaerobic bacteria and standard methods were recruited for physicochemical analysis. The results of physicochemical analysis of water samples were found to be in conformity with the standard regulations. Microbiological results of water, however, did not comply with required standards since the findings showed 30% enterococci, 12% coliform, 24% sulphite reducing anaerobic and 8% E. coli. The mean values of pH, Na and Mg, and presence of bacteria except E. coli were higher in autumn than spring. Effect of residential areas on aerobic plate count, coliform and E. coli were found to be insignificant (p>0.05), the effects on all other variables were found to be significant (p<0.05).
Typing of Verotoxigenic Escherichia Coli Strains Isolated From Animal and Human Sources
(Kafkas Univ, veteriner Fakultesi dergisi, 2009) Gulhan, Timur; Boynukara, Banur; Alisarli, Mustafa
In this study, the verotoxin types of 73 E. coli strains, 58 animal origin and 15 human origin, were examined by Vero cell culture technique. Typing was performed using Duopath verotoxin GLISA test tool. Of them 67 (91.8%) strains were typed; 31 (42.5%) were VT1, 20 (27.4%) were VT2 and 16 (21.9%) were VT1+VT2. 1 animal origin and 5 human origin totally 6 (8.2%) strains could not be typed. It was seen that this technique used in determining the verotoxin types of human and food origin E. coli strains of can also be used as practical test in determining the verotoxin types of animal origin E. coli strains.