Browsing by Author "Alizade, Ares"

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The Effects of Mesenchymal Stem Cells Applied During the Subacute Period in Peripheral Neuropathy
(Cukurova Univ, Fac Medicine, 2024) Kiroglu, Olcay; Maytalman, Erkan; Alizade, Ares; Emre, Mustafa; Zorludemir, Suzan; Aksu, Fazilet
Purpose: The study aims to investigate the effect of bone marrow-derived mesenchymal stem cells (BM-MSCs) administered subacute period to neuropathic mice on allodynia and nerve-muscle tissue functions during 24 weeks. Materials and Methods: Peripheral neuropathy was induced by partial sciatic nerve ligation. Experiments were conducted in Control, Sham, Neuropathic, BM-MSC, and Neuropathic+BM-MSC groups. Allodynia was measured by cold plate test at the 2nd, nd , 6th, th , and 24th th weeks. Electrophysiological and histopathological examinations were performed on isolated nerve-muscle tissues at the end of the 24th th week. Results: Allodynia threshold increased in the Neuropathic+BM-MSC group (7.76 +/- 0.33 sec) from the 6 th week and continued to increase along 24 weeks compared to the Neuropathic group (4.36 +/- 0.21 sec). Action potential (137.9 +/- 7.85 mV) and depolarization (0.74 +/- 0.01 msec) values of the Neuropathic+BM-MSC group exhibited partial improvement compared to the Neuropathic group (121.5 +/- 3.03 mV and 0.81 +/- 0.02 msec, respectively) at the 24th th week. Muscle tissue's resting membrane potential values increased in the Neuropathic+BM-MSC group compared to the Neuropathic group (-73.4 +/- 0.2 and-87.7 +/- 0.2 mV, respectively). Histopathological examination of nerve tissue revealed loss of myelinated axons and significant fibrosis in the endoneurium in the Neuropathic group while Schwann cell proliferation and preservation of myelinated axons were observed in the Neuropathic+BMMSC group. Muscle fiber atrophy, compensatory hypertrophic fibers, and increased central nuclei were seen in the Neuropathic group, while small atrophic muscle fiber groups were identified in the Neuropathic+BM-MSC group. Conclusion: BM-MSC application in the subacute period is found to reduce allodynia and provide functional recovery in nerve-muscle tissue in experimental peripheral neuropathy.
Hypericin and Resveratrol Anti-Tumor Impact Through E-Cadherin, N-Cadherin, Galectin-3, and BAX/BCL-ratio; Possible Cancer Immunotherapy Succor on Y79 Retinoblastoma Cells
(Wiley, 2025) Shahriari, Fatemeh; Barati, Mojdeh; Shahbazi, Sahba; Arani, Hamid Zaferani; Dahmardnezhad, Mozhgan; Javidi, Mohammad Amin; Alizade, Ares
One of the detrimental features of retinoblastoma is highly invasiveness of this type of cancer; the ability to metastasize into distal organs even in the early stages. This phenomenon highlights the importance of experiments targeting this characteristic to diminish the disquieting outcomes. In this study we assessed the impact of hypericin, and resveratrol (two main herbal extracts with known anti-cancer properties on the important metastasis/cancer progression pathways in Y79 retinoblastoma cell line). MTT assay performed for 24 and 48 h, and the kind of cell death, investigated by Annexin V/PI flowcytometry. Finally, the expression of important metastatic/apoptotic genes assessed in different samples by the aid of real-time PCR. The 24 and 48 h IC50 for resveratrol, and hypericin was about 10.87 and 7.697, 2.267, and 1.732 mu g/mL, respectively. Flowcytometry results revealed that the kind cell death after treatment of Y79 cell with both compounds was mostly apoptosis. Both compounds induced up-regulation of E-cad, and down-regulation of N-cad, and Gal-3; furthermore, BAX/BCL-2 ratio increased significantly in cells treated with hypericin. Hypericin seems to exert more cytotoxicity effect on Y79 cells compared with resveratrol. As both compounds enhanced the expression of the E-cadherin, along with the decreasing the galectin-3, they can be proposed as beneficial accompanying reagents in cancer immunotherapy.
In Vitro Apoptotic and Antiproliferative Effects of Propranolol on Human Breast Cancer Cells
(Natl inst Science Communication-niscair, 2024) Alizade, Ares; Terzi, Menderes Yusuf
Breast cancer is an issue of concern with increasing incidence among women worldwide. Propranolol, as an antihypertensive drug, exerts anticancer effects too. We conducted this study to analyze the in vitro apoptotic and antiproliferative effects of propranolol in human MCF-7 breast cancer cells. MCF-7 cells were seeded into 6 -well plates and treated with 50 mu L propranolol for 24 hours. After cell homogenization, the levels of pro-apoptotic proteins BCL2 associated X (BAX), apoptosis inducing factor (AIF), C/-EBP homologous protein (GADD153), and glucose -regulated protein 78 (GRP78), anti-apoptotic protein BCL2 apoptosis regulator (BCL-2), and cycle -regulator WEE1 G2 checkpoint kinase (WEE1) were measured with ELISA. Propranolol significantly upregulated pro-apoptotic proteins AIF, BAX, GADD153, and GRP78 while downregulated anti-apoptotic protein BCL2. The level of WEE1, as the main regulatory cell cycle protein at the G2/M checkpoint, significantly increased after propranolol treatment. Propranolol inhibited the proliferation of MCF-7 human breast cancer cells by upregulating pro-apoptotic factors AIF, BAX, GADD153 and GRP78 and by downregulating antiapoptotic BCL2. Elevated WEE1 levels after propranolol treatment might lead the tumor cells into a sustained cell -cycle arrest which eventually resulted in caspase-dependent or -independent mitochondrial or endoplasmic-reticulum stress -induced apoptosis. So, propranolol can be utilized as a potential therapeutic agent in breast cancer therapy.
In Vitro Effects of Antioxidant and Proapoptotic Activities of Thymoquinone Iron Complex
(Dokuz Eylul Univ inst Health Sciences, 2022) Ozbolat, Guluzar; Alizade, Ares
Introduction: This study aimed to investigate the proapoptotic and antioxidant effects of the Thymoquinone (TQ) iron complex on the SW480 cell line. This study investigates the proapoptotic and antioxi-dant effects of the TQ iron complex on the SW480 cell line. Material and Methods: The SW480 cells were routinely cultured in a medium for 48 h. and incubated at 37 degrees C in a 5% CO2 in the air. After the incubation period, the cells were washed with buffer, and 100 ml of the denaturing lysis buffer per 0.5 was added to 2x10(7) cells for 15 min, and supernatants were tak-en. ELISA test was used to examine the expression and activity of GADD153, Wee1, cleaved Caspase-3, Bax, GRP78, and Bcl-2 proteins in SW480 cells. In this study, to measure activities of total antioxidant capacity (TAS), catalase (CAT), total oxidant capacity (TOS), and superox-ide dismutase (SOD) activities were investigated by the ELISA method in cell lines SW480 treated with the TQ iron complex. Results: ELISA test results indicated that the activities of apoptotic proteins Bax, Wee1 Caspase-3, GADD153, GRP78, and Bcl-2 in human SW480 cell lines were significantly increased in the 48-hour treatment. Conclusion: Our results of this study demonstrated that in untreated cultures, high TAS, SOD and CAT ac-tivities were found in SW480 cell lines than in control cell lines.
In Vivo Assessment of a Capsaicin-Containing Microemulsion for Neuropathic Pain Management
(Galenos Publ House, 2025) Shayyah, Rumooz; Bolgen, Umay Merve Guven; Alizade, Ares; Kayiran, Serpil Demirci; Ebrahimi, Sonia; Aksu, Fazilet
BACKGROUND/AIMS: Capsaicin (CAP) has been used in clinical applications for the treatment of neuropathic pain (NP). The main disadvantages of CAPare itsshort half-life, lowwatersolubilityand low bioavailability. Thisstudy intended to producea therapeutically effective microemulsion (ME) formulation comprising CAP to decrease NP. MATERIALS AND METHODS: ME was prepared using oleic acid, Tween 80, propylene glycol, ethanol, and water. Neuropathy was induced by partial sciatic nerve ligation (PSL) in mice. Two weeks after, PSL NP was tested using the cold plate (CP) and von Frey (VF) tests. The mice in the treatment group were administered 10 mg/kg CAP by oral gavage. The effects on NP of conventional CAP and ME CAP were compared. RESULTS: The prepared ME formulation of (CAP) was a homogeneous, transparent, thermodynamically stable dispersion of water and oil. The classic CAP was not effective on NP, while ME CAP was effective in the CP. The ME CAP was more effective on mice, than classic CAP using the VF test. CONCLUSION: The developed novel ME formulation at lower doses could reduce side effects and improve the bioavailability of the oral administration of CAP in the treatment of NP, and thus, would achieve good patient compliance.
Investigation of Behavioral Changes and Histopathological Changes in the Brain in Alzheimer's Modeled Mice With Aluminium Chloride (AlCl3)
(Galenos Publ House, 2025) Kanan, Derya Deniz; Aydemir, Isil; Alizade, Ares; Ebrahimi, Sonia; Aksu, Fazilet
BACKGROUND/AIMS: Aluminium (Al) is related with many brain diseases including Alzheimer's disease, however the relation between Al and neurodegenerative diseases is still controversial. In this study behavioral and histopathological changes were investigated in the AlCl3 induced Alzheimer's model in mice. MATERIALS AND METHODS: Male and female mice divided into control (tap water) and test (50 mg/kg/day AlCl3) groups. After ninety days of AlCl3/water intake, rota rod, elevated plus maze, Passive Avoidance tests were performed to assess motor coordination, spatial and emotional learning and memory, respectively. After the behavioural experiments, histopathological examination was made in the brain. RESULTS: No difference was found between the groups and the genders in the rota-rot test. Learning and memory were impaired in both gender. Long term memory impaired female mice in the test group. Neuron loss was observed in both the cerebral cortex and hippocampus CA1 regions in the test group, while an increase in pycnotic nucleated cells was observed. Neurofibrillary tangles were also observed in the hippocampus, with neurons with basophilic nuclei prominently. CONCLUSION: As a result, long-term exposure to low doses of aluminum may cause behavioral changes and histopathological changes in the brain.