Browsing by Author "Alkan, Elif Ebru"

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Effect of Isgin (Rheum Ribes L.) on Biochemical Parameters, Antioxidant Activity and Dna Damage in Rats With Obesity Induced With High-Calorie Diet
(Taylor & Francis Ltd, 2023) Bati, Bedia; Celik, Ismail; Turan, Abdullah; Eray, Nese; Alkan, Elif Ebru; Zirek, Ali Kemal
The present study was designed to investigate the effects ofRheum ribesL. plant root extracts on DNA damage, biochemical and antioxidant parameters in rats with experimental obesity induced with a high-calorie diet. The study groups were divided as "normal control(NC)", "obese control(OC)", "obese +Rheum ribes(OR1)(200 mg/kg)" and "obese +Rheum ribes(OR2)(400 mg/kg)". At the end of the application, rats were sacrificed and blood and tissue samples were obtained. According to the results obtained, the marker of DNA damage in tissues of 8-OHdG was determined to be significantly reduced in brain tissue of the OR1 and OR2 groups compared to the NC group. However, fluctuations were identified in the MDA activity, antioxidant defense system elements and serum biomarkers in tissues. In conclusion,Rheum ribesplant root extract ensured improvements in DNA damage in brain tissues and MDA levels and showed positive effects on antioxidant parameter activities in different tissues.
Histopathological and Immunohistochemical Study of Antidiabetic Effects of Heracleum Persicum Extract in Experimentally Diabetic Rats
(Assoc Pharmaceutical Teachers india, 2017) Yaman, Turan; Uyar, Ahmet; Celik, Ismail; Alkan, Elif Ebru; Keles, Omer Faruk; Yener, Zabit
Objective: This study aims to investigate the antioxidant properties and protective effects of Heracleum persicum (HP) extract in streptozotocin (STZ)-induced diabetic rats. Material and Methods: Forty-two Wistar albino male rats were divided into six groups including Control (C); Diabetes mellitus (DM); DM+Akarboz 20 mg/kg; DM+100 mg/kg HP extract (HP1); DM+200 mg/kg HP extract (HP2) and DM+400 mg/kg HP extract (HP3). Experimental diabetes was established by a single-dose [45 mg/kg, intra-peritoneal (i.p)] STZ injection. Essential dosages of HP extracts and Akarboz were applied with gastric gavage for 21 day. Results: In histopathological evaluation of the stained liver and kidney sections of diabetic rats showed degeneration and necrosis of hepatocytes, inflammatory cell infiltration and hydropic degeneration and necrosis in tubulus epithelial cells, disorder of glomerular structure and lymphocyte infiltration. These histopathological changes were ameliorated in the HP-treated rats depending on the dose level. Glutathione peroxidase 1 (GPx-1) immunoreactivity was detected in hepatocytes of liver and tubule epithelial cells of kidney. We have shown that treatment with extracts of HP modulates GPx-1 expression in HP-treated rats. STZ-induced degenerative changes in beta-cells caused decreases in the number of functioning beta-cells and insulin immunoreactivity in the pancreas of the diabetic rats. The pancreas of HP-treated rats were improved and the number of immunoreactive beta cells were significantly increased. Conclusion: Our data suggests that the STZ-induced immunohistochemical and histopathological alterations could be prevented by HP extract probably due to possess the ability to regenerate beta-cells.
Protective Effects of Bryonia Multiflora Extract on Pancreatic Beta Cells, Liver and Kidney of Streptozotocin-Induced Diabetic Rats: Histopathological and Immunohistochemical Investigations
(Assoc Pharmaceutical Teachers india, 2017) Uyar, Ahmet; Yaman, Turan; Kele, Omer Faruk; Alkan, Elif Ebru; Celik, Ismail; Yener, Zabit
Objective: In this study, the ameliorative potential and antioxidant capacity of treated with different doses of Bryonia multiflora extract (BE) was investigated using histopathological and immunohistochemical changes in pancreatic beta cells, liver and kidney tissues of streptozotocin (STZ)-induced diabetic rats. Material and Methods: A total of forty-two healthy adult Wistar albino male rats were divided randomly into six groups as Control (C); Diabetes mellitus (DM); DM+Akarboz 20 mg/kg; DM+100 mg/kg BE extract (BE1); DM+200 mg/kg BE extract (BE2); DM+400 mg/kg BE extract (BE3). Experimental diabetes was established by a single-dose [45 mg/kg, intra-peritoneal (i.p)] STZ injection. Essential dosages of BE extracts and Akarboz were applied with gastric gavage for 21 day. Blood glucose levels were recorded throughout the all experiment period. Results: Histopathological studies showed that hepatorenal and pancreatic protection by depending on the dose level of BE extracts was further supported by the almost normal histology in DM+BE extract-treated group as compared to the degenerative changes such as disorder of architectural structure, inflammatory cell infiltration, hydropic degeneration and necrosis in pancreas, liver and kidney tissues of STZ-treated rats. Immunohistochemical investigation revealed that STZ-induced degenerative changes in beta-cells in the pancreas of the diabetic rats has reduced insulin immunoreactivity. On the other hand, insulin immunoreactivity in the beta cells of pancreas of BE -treated diabetic rats has significantly increased. Additionally, Glutathione peroxidase 1 (GPx1) immunoreactivity was lower in the tissues of diabetic rats (DM group) compared to the other groups. Conclusion: In conclusion, BE extract has a protective effect on tissue damage probably due to its antioxidant activity and possess the ability to regenerate beta-cell in STZ-induced diabetic rats.
The Protective Role of Urtica Dioica Seed Extract Against Azoxymethane-Induced Colon Carcinogenesis in Rats
(Routledge Journals, Taylor & Francis Ltd, 2022) Uyar, Ahmet; Dogan, Abdulahad; Yaman, Turan; Keles, Omer Faruk; Yener, Zabit; Celik, Ismail; Alkan, Elif Ebru
The aim of this study was to investigate the protective role of Urtica dioica seed (UDS) extract against azoxymethane (AOM)-induced colon carcinogenesis in rats. Thirty-two male Wistar albino rats were divided into four groups: Control, AOM, AOM + UDS, and UDS. The AOM and AOM + UDS groups were induced by AOM (15 mg/kg body weight) subcutaneously once a week for 10 weeks. AOM + UDS and UDS groups additionally received fed with pellets included 30 ml/kg UDS extract. At the end of the trial, blood and colon tissue samples were taken from the rats following necropsy. The gross and histopathological findings revealed that the administration of UDS extract significantly decreased lesions including aberrant cript foci, adenoma, and adenocarcinoma formation both numerically and dimensionally. Immunohistochemically, slight CEA and COX-2, strong Caspase-3 immune-expressions were detected in the group AOM + UDS compared to AOM group. Biochemical examinations indicated that a markedly increase in the malondialdehyde and fluctuated antioxidant defense system constituents levels such as reduced glutathione, glutathione s-transferase, glutathione peroxidase, superoxide dismutase were restored in AOM + UDS group. These results reveal that the UDS may act as a chemopreventive dietary agent, inducing apoptosis, resulting in a significant reduction of colon carcinogenesis.
Reduction of Hepatorenal and Pancreatic Damage by Ferula Elaeochytris Extract in Stz Induced Diabetic Rats
(Taylor & Francis Ltd, 2021) Uyar, Ahmet; Yaman, Turan; Keles, Omer Faruk; Alkan, Elif Ebru; Demir, Abdulbaki; Celik, Ismail; Yener, Zabit
The therapeutic potential and antioxidant capacity of Ferula elaeochytris extract (FE) in the liver, kidney and pancreas of rats with diabetes induced by streptozotocin (STZ) was assessed using biochemistry, histopathology and immunohistochemistry. Forty adult Wistar albino male rats were divided randomly into five groups of eight rats each. The normal control (NC) group was untreated. The diabetes control (DC) group was treated with STZ to induce diabetes. The diabetes + acarbose group (DAC) was treated with STZ, then with acarbose daily for 28 days. The diabetes + FE (DFE) group was treated with STZ, then FE daily for 28 days. DC rats had inflammatory cell infiltration, hydropic degeneration and necrosis, whereas the DFE rats exhibited nearly normal histology. Insulin immunostaining in the pancreatic beta cells was decreased in the DC group compared to the NC group, whereas the DFE group was similar to the NC group. Many serum biomarkers of damage to liver, kidneys or pancreas were elevated in the DC group compared to the NC group; these biomarkers were decreased in the DFE group. The DC group exhibited increased malondialdehyde levels and decreased levels of the antioxidant defense system constituents compared to the NC group. The level of biomarkers the DFE group was close to the NC group. FE exhibited a protective effect against tissue damage owing to its antioxidant activities and to its ability to effect regeneration of beta-cells in STZ induced diabetic rats.
The Indication of Performance Level of Experimental Activities Which Are Included in Science and Technology Teaching Schedule in Secondary
(2011) Alkan, Elif Ebru; Selçuk, Ahmet
Bu araştırma, Fen ve Teknoloji Dersi Öğretim Programında yer alan deneysel etkinliklerin yapılma düzeylerini tespit etmek, etkinliklerin amacına ulaşıp ulaşmadığını saptamak ve etkinliklerin yapılmama nedenlerini belirlemek amacıyla 2009-2010 eğitim-öğretim yılında, iki ayrı dönemde ilköğretim ikinci kademede Van il merkezinde görev yapan 150 Fen ve Teknoloji öğretmeni ile yapılmıştır. Ayrıca 15 Fen ve Teknoloji öğretmenine de yarı yapılandırılmış mülakat soruları yönlendirilmiştir.Çalışmada elde edilen bulgular değerlendirilirken, istatistiksel analizler için SPSS for Windows 17.0 programı kullanılmıştır. Çalışma verileri değerlendirilirken tanımlayıcı istatistiksel metotları olan, Frekans, Yüzde, Ortalama ve Standart sapma kullanılmıştır.Niceliksel verilerin karşılaştırılmasında iki grup olduğu durumlarda, gruplar arası karşılaştırmalarda Bağımsız örnekler t testi kullanılmıştır. İkiden fazla grup durumunda parametrelerin gruplar arası karşılaştırmalarında Tek yönlü Anova testi kullanılmıştır.Anket sonuçlarına göre;Araştırmaya katılan öğretmenlerin etkinlikleri yapma oranı ve etkinliğin amacına ulaşma oranı ortalamalarının, cinsiyet değişkenine göre, kıdem değişkenine göre, fakülte değişkenine göre ve lisansüstü eğitim değişkenine göre grup ortalamaları arasındaki fark istatistiksel açıdan anlamlı bulunamamıştır.Araştırmaya katılan 6.sınıf öğretmenlerinin ortalama etkinlik yapma sayısı 24; toplam etkinlik içinde etkinlik yapma oranları %68, 7.sınıf öğretmenlerinin ortalama etkinlik yapma sayısı 18; toplam etkinlik içinde etkinlik yapma oranları %64 ve 8.sınıf öğretmenlerinin ortalama etkinlik yapma sayısı 26; toplam etkinlik içinde etkinlik yapma oranları %70 olarak bulunmuştur.Araştırmada; deneysel etkinliklerin yapılamama nedenleri arasında daha çok sınıfların kalabalık oluşu, okullardaki araç-gereç eksikliği ve zamanın yetersiz oluşu gösterilmiştir. Ayrıca öğrenci seviyesinin üzerinde olması nedeniyle veya öğretmen tarafından gerekli bulunmadığı için de büyük oranda yapılmayan etkinlikler olduğu saptanmıştır.Anahtar Kelimeler: Deneysel etkinlik, Fen ve teknoloji müfredat programı, İlköğretim, Öğretmen
The Therapeutics Effects and Toxic Risk of Heracleum Persicum Desf. Extract on Streptozotocin-Induced Diabetic Rats
(Elsevier, 2018) Alkan, Elif Ebru; Celik, Ismail
There is an increasing interest against to fight of diabetes by using hypoglycemic plants in the world. The public thinks that Heracleum persicum (HP) has antidiabetic effect local consumer in Turkey. As far as our literature survey, no studies have been reported so far on antidiabetic effects and toxic risk potential of the HP lyophilized extract supplementation used in this study. The aim of this study, for the first time, was to investigate the therapeutic effects of diabetic complications, antioxidant properties and toxic risk potential of HP against experimentaly streptozotocin (STZ) induced diabetes in rats, which were evaluated by measuring the level of serum biomarker released diabetes complications changes such glucose, insulin, c-peptide, lipid profile (LP), hepatic and renal damage biomarkers (HRDB), glucosylated hemoglobin (HbA1c), antioxidant defense system constituents (ADSCs), malondialdehyde (MDA) content measured in erythrocyte, brain, kidney and liver tissues, and alpha-glucosidase activitiy of small intestine. The plant aqueous extract was allowed to freeze-dried under a vacuum at - 54 degrees C to obtain a fine lyophilized extract. The study was performed on STZ-induced diabetic rats (45 mg/kg, body weight (bw), intraperitonally) designed as normal control (NC), diabetic control (DC), diabetes + acarbose (DAC) (20 mg/kg, bw), diabetes + HP (100 mg/kg, bw) (DH1), diabetes + HP (200 mg/kg, bw) (DH2) and diabetes + HP (400 mg/kg, bw) (DH3)] groups. The experimental process lasted 21 days. According to results; the levels of blood glucose (BG), glucosylated hemoglobin (HbA1c) and malondialdehyde (MDA) of DC group increased significantly (p < 0.05) compared to NC group, whereas these parameters of the groups treated with oral administrations of HP plant lyophilized extract were observed significant (p < 0.05) declines compared to DC. The biochemical analyses showed a considerable decrease in insulin and c-peptide levels and the fluctuated ADSCs in the DC group as compared to control group, whereas the extract supplementations diet restored the diabetic complications parameters towards to the NC. On the other hands, liver damage serum enzymes as serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels were incressed significantly (p < 0.05) in the plant extract supplementations groups as compared to NC and DC groups. It was concluded that while the extracts of HP have had therapeutic effects on some complications caused by diabetes, but might be caused hepatocyte damage changes as the transport functions and membrane permeability of these cells, thus causing enzymes to leak.