Browsing by Author "Allahverdiyev, Oruc"

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Analysis of Phytochemical Composition and Biological Activities of Verbascum Cheiranthifolium Var. Cheiranthifolium Stem and Flowers
(2018) Bengü, Aydın Şükrü; Dalar, Abdullah; Allahverdiyev, Oruc
Within this study phytochemical composition, antioxidant and enzymeinhibitory activities of extracts obtained from stem and flower of Verbascumcheiranthifolium var. cheiranthifolium were analysed. Both of the extracts weredetected as rich sources of phenolics (verbascoside and luteolin hexoside), variousvolatile and fatty acid compounds. Luteolin hexoside rich stem extract hadpronounced FCR, FRAP and α-glucosidase inhibitory activities. Flower extract hadhigh levels of ORAC assay and effectively suppressed activity of pancreatic lipaseenzyme, which was rich in verbascoside compound. Phenolic compounds andvolatile compounds present in the extracts might be the main contributors ofantioxidant capacity and enzyme inhibitory activities of the stem and flowerextracts. Pronounced antioxidant and enzyme inhibitory activities and richbioactive composition determined in this study reveal that Verbascumcheiranthifolium var. cheiranthifolum extracts might be a good source for naturalhealth attributing sources.
Can Juniperus Communis L. Oil Improve Nephropathy in Diabetic Rats
(Marmara Univ, inst Health Sciences, 2020) Kahraman, Tahir; Berkoz, Mehmet; Allahverdiyev, Oruc; Mahmood, Evan Abdulkarim; Yildirim, Metin; Yalin, Serap
Objective: Juniperus communis L. (J. communis) is a shrub belonging to family Cupressaceae L. mainly growth in Eurasia. The antioxidant and antidiabetic activity of aqueous extract of J. communis L. berries indicated benefits as a potent antidiabetic in streptozotocin induced diabetic albino rats. This study was carried out to determine whether J. communis L. oil supplement will effectively manage renal dysfunction in diabetic rats. Methods: Twenty eight rats were divided into 4 equal groups as follows; control group, diabetic group (45 mg/kg, i.p. streptozotocin), J. communis L. oil (200 mg/kg) treated group, and diabetic+J. communis L. oil (200 mg/kg) treated group. At the end of the experimental period, all rats were sacrificed and renal function parameters such as kidney antioxidant and lipid peroxidation markers and serum glucose, HbA1c, creatinine, serum urea, blood urea nitrogen (BUN), and serum total protein levels were measured in all groups. Results: HbA1c, serum glucose, urea, creatinine, BUN and, kidney lipid peroxidation levels increased (p<0.05), but serum total protein and antioxidant levels decreased in diabetic group comparing with control group (p<0.05). Furthermore, HbA1c, serum glucose, urea, creatinine and BUN and, kidney lipid peroxidation levels decreased and also, serum total protein and antioxidant levels increased in diabetic group treated with J. communis L. oil comparing with diabetic group (p<0.05). Conclusion: This study has provided that J. communis L. oil provide a protective effect on the kidney as evidenced by an improvement of the renal function tests as well as reduction in oxidative stress parameters in experimental diabetic nephropathy model.
Hepatoprotective Effect of Nigella Sativa L. Extract in Methyl Parathion Exposed Rats
(Parlar Scientific Publications (p S P), 2019) Berkoz, Mehmet; Kahraman, Tahir; Yildirim, Metin; Yigit, Muhammet Faruk; Allahverdiyev, Oruc
Methyl parathion (MP) is an organophosphorus pesticide that can induce hepatotoxicity in living organisms. Although many plant extracts have been utilized againts MP induced liver injury, it has not yet known whether Nigella sativa L. extract (NSE) has protective effects or not. We aim to investigate the hepatoprotective effect of NSE through the oxidative stress and anti-inflammatory pathways. A total of 28 rats were divided into 4 groups; control, MP, NSE and MP + NSE. We measured liver MDA level and antioxidant parameters, hepatic function enzyme, plasma cytokine levels, liver MPO activity, liver iNOS and COX-2 mRNA levels and MAPK/NF-kappa b protein levels. Our data showed that MP increased hepatic function tests, liver MDA level and MPO activity, plasma proinflammatory cytokines, liver iNOS and COX-2 rnRNA and MAPKs protein levels and decreased antioxidant parameters, antiinflammatory cytokine and liver NF-kappa b protein levels. NSE decreased hepatic function tests, liver MDA level and MPO activity, plasma proinflammatory cytokines, liver iNOS and COX-2 mRNA and MAPKs protein levels and increased antioxidant parameters, anti-inflammatory cytokine and liver NF-kappa b protein levels in the MP treated rats. These results suggested the antiinflammatory and antioxidative function of NSE in MP induced liver injury.
Hiperforin ve Hiperisinin Raw 264.7 Makrofajında İnflamatuar Yanıt Üzerine Etkisinin İncelenmesi
(2018) Berkoz, Mehmet; Yıldırım, Metin; Allahverdiyev, Oruc
Amaç: Bu çalışma in vitro model kullanarak hiperisin ve hiperforinin anti-inflamatuvar özelliğini belirlemeyi amaçlamaktadır. Gereç ve Yöntem: Bu çalışmada, lipopolisakkarit (LPS) ile uyarılmış RAW 264.7 makrofajları, 0, 25, 50, 75 ve 100 nM hiperforin ve 0, 2,5, 5, 7,5 ve 10 μM hiperisin ile muamele edilmiştir. LPS ile indüklenmiş RAW 264.7 makrofajlarında, prostaglandin E2 (PGE2) ve nitrik oksit (NO) üretimi ile siklooksijenaz-2 (COX-2) ve indüklenebilir nitrik oksit sentaz ( iNOS) gen ekspresyonunu ölçerek hiperisin ve hiperforinin antienflamatuar etkilerini göstermeyi amaçladık. Bulgular: Yaptığımız çalışma, uygulanan tüm hiperforin ve hiperisin konsantrasyonlarının, RAW 264.7 makrofajlarında nitrik oksit (NO) üretimini ve indüklenebilir nitrik oksit sentaz ( iNOS) ve siklooksijenaz 2 (COX-2) gen ekspresyonlarını önemli ölçüde azalttığını göstermektedir. Benzer bir şekilde, uygulanan tüm hiperforin ve hiperisin konsantrasyonlarının prostaglandin E2 (PGE2) üretimini azalttığı ancak bu düşüşün sadece 75 ve 100 nm hiperforin ile 7,5 ve 10 μM hiperisin uygulanan gruplarda istatistiksel olarak anlamlı olduğu görülmüştür. Sonuç: Bu durum, hiperforin ve hiperisinin doza bağımlı bir şekilde RAW 264.7 makrofajlarındaki NO ve PGE 2 üretimini ve iNOS ve COX-2 gen ekspresyonunu inhibe ettiğini göstermektedir. Bu nedenle, hiperisin ve hiperforinin inflamatuar yanıtı azaltmak için kullanılabileceğini söylemek mümkündür.
Rosa Canina L. Ethanolic Extract Induces the Anti-Proliferative and Apoptosis Potential in Mcf-7 and Mda-Mb Cell Lines
(Parlar Scientific Publications (p S P), 2019) Berkoz, Mehmet; Ozkan-Yilmaz, Ferbal; Ozluer-Hunt, Arzu; Yildirim, Metin; Allahverdiyev, Oruc; Aslan, Ali
Rosa canina L. (rose hip) fruits have been used for their diuretic, laxative, anti-gout, anti rheumatism properties in traditional medicine. Rose hip berries contain a variety of components such as flavonoid. The previous studies showed that flavonoid has anti-cancer properties. Tha aim of this study is to evaluate and screen the effect of apoptosis and the anticancer potential of rose hip ethanolic extract on human breast cancer cell lines; MCF-7 and MDA-MB-468. The anti-proliferative activity of rose hip extract was evaluated using MTT, flowcytometry by annexin V/PI double staining, and caspase-3 activity. The results of MTT showed that the EDso of both human breast cancer cell lines was 25 g/mL of rose hip extract, 48 hours after treatment. Flowcytometry by annexin V/PI showed that rose hip extract induced late apoptosis in MCF7 and early apoptosis in MDA-MB-468. In addition, the caspase-3 colorimetric method showed that caspase-3 increased in the MDA-MB-468 after treatment with rose hip extract. As a result, the ethanol of rose hip ethanolic extract induced apoptosis in both human breast carcinoma cell lines.
Thymoquinone Reduces the Inflammatory Response in Adipocytes
(Wiley-blackwell, 2016) Berkoz, Mehmet; Allahverdiyev, Oruc; Yildirim, Metin; Yalin, Serap